{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:29:12Z","timestamp":1787027352216,"version":"build-2736575974"},"reference-count":26,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013138","name":"Hainan Provincial Department of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013138","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012477","name":"Fundamental Research Funds for the Key Research Program of Chongqing Science and Technology Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012477","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Public Health"],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>This study aimed to establish a rapid detection method for SARS-CoV-2 by integrating loop-mediated isothermal amplification (LAMP) with lateral flow dipstick (LFD) technology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>Specific primers targeting the nucleocapsid (N) gene and open reading frame 1ab (ORF1ab) gene of SARS-CoV-2 were designed and labeled with 6-FAM and biotin, respectively. After systematically optimizing key reaction parameters, including primer selection, primer concentration, and LAMP reaction time, the sensitivity, specificity, and clinical applicability of the method were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The proposed LAMP-LFD assay enables rapid detection within 30 min with high specificity, accurately identifying the N and ORF1ab genes of SARS-CoV-2 without cross-reactivity with influenza A hemagglutinin gene, influenza B neuraminidase gene, and respiratory syncytial virus M gene. The limit of detection reached 1.892 \u00d7 10\n                      <jats:sup>1<\/jats:sup>\n                      copies\/\u03bcL, showing comparable sensitivity to agarose gel electrophoresis and real-time quantitative reverse transcription PCR (RT-qPCR). The results were consistent across different batches of primers and probes, demonstrating good reproducibility. When compared with RT-qPCR using 114 inactivated throat swab samples, the diagnostic agreement rate reached 95.61%.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>This study provides technical support for the surveillance and control of SARS-CoV-2 infection.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fpubh.2026.1798338","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T16:17:23Z","timestamp":1774369043000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["LAMP-LFD: establishment and evaluation of a new diagnostic platform for SARS-CoV-2 rapid detection"],"prefix":"10.3389","volume":"14","author":[{"given":"Qizhi","family":"Diao","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Medicine, Sanya Women and Children's Hospital Affiliated to Hainan Medical University","place":["Sanya, China"]},{"name":"Department of Clinical Laboratory Medicine, Hainan Branch, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University","place":["Sanya, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Hainan Medical University","place":["Haikou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine, Hainan Branch, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University","place":["Sanya, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajia","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory 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