{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:01:10Z","timestamp":1790834470712,"version":"4.1.0"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Public Health"],"abstract":"<jats:p>\n                    Human adenovirus (HAdV) is an important respiratory virus in children, but sequence-level evidence for HAdV-related signals in lower respiratory tract samples remains limited. In this study, we analyzed metagenomic next-generation sequencing data from 189 bronchoalveolar lavage fluid (BALF) libraries from pediatric and adult clinical cohorts, including 30 pediatric and 159 adult patients, together with laboratory environmental controls. MEGAN read-level classification was used to screen for\n                    <jats:italic>Adenoviridae<\/jats:italic>\n                    -related signals. HAdV-related contigs were further reviewed, and a BALF-derived HAdV-B-related representative contig was selected for read recruitment and coverage analysis. Phylogenetic trees were reconstructed using penton base, hexon, and fiber protein sequences.\n                    <jats:italic>Adenoviridae<\/jats:italic>\n                    -classified signals were more apparent in pediatric BALF libraries than in adult BALF libraries or environmental controls. Several pediatric BALF libraries showed broad read coverage across the HAdV-B-related representative contig, whereas adult libraries and environmental controls mainly showed low-coverage-breadth signals. Phylogenetic analysis placed BALF-derived penton base, hexon, and fiber sequences within HAdV-B-related lineages. Twenty-seven capsid protein sequences from 12 pediatric samples had best BLASTp matches to references annotated as HAdV-B3 (\n                    <jats:italic>n<\/jats:italic>\n                    \u202f=\u202f23) or HAdV-B7 (\n                    <jats:italic>n<\/jats:italic>\n                    \u202f=\u202f4). These findings provide sequence evidence for HAdV-B-related sequence signals in clinically selected pediatric BALF samples and support further investigation using targeted assays and genome sequencing.\n                  <\/jats:p>","DOI":"10.3389\/fpubh.2026.1961482","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:41:59Z","timestamp":1790833319000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Viral metagenomic characterization of HAdV-B-related signals in bronchoalveolar lavage fluid from pediatric and adult clinical cohorts"],"prefix":"10.3389","volume":"14","author":[{"given":"Chunyan","family":"Zhao","sequence":"first","affiliation":[{"name":"School of Health and Nursing, Wuxi Taihu University","place":["Wuxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaheng","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Medicine, Jiangsu University","place":["Zhenjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Medicine, Jiangsu University","place":["Zhenjiang, China"]},{"name":"Department of General Surgery, Wujin Hospital Affiliated with Jiangsu University","place":["Changzhou, Jiangsu Province, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Medicine, Jiangsu University","place":["Zhenjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songyi","family":"Ning","sequence":"additional","affiliation":[{"name":"School of Medicine, Jiangsu University","place":["Zhenjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,10,1]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"e25","DOI":"10.1093\/cid\/cir531","article-title":"The management of community-acquired pneumonia in infants and children older than 3 months of age: clinical practice guidelines by the Pediatric Infectious Diseases Society and the Infectious Diseases Society of America","volume":"53","author":"Bradley","year":"2011","journal-title":"Clin Infect Dis"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"e45","DOI":"10.1164\/rccm.201908-1581ST","article-title":"Diagnosis and treatment of adults with community-acquired pneumonia. 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