{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T05:04:58Z","timestamp":1789362298975,"version":"build-2803163510"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Aging"],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Nothobranchius furzeri<\/jats:italic>\n                      , commonly known as African turquoise killifish, has emerged as a powerful model organism in diverse biological research areas owing to its exceptionally short lifespan of 4\u20106 months. Dietary choices can significantly impact the physiology of this fish and its median longevity, prompting considerations on live versus dry food and feeding frequencies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Here, we present a novel, cost\u2010effective, and modular automated feeding system for\n                      <jats:italic>N. furzeri<\/jats:italic>\n                      , capable of delivering up to 36 feedings per day with high precision. Using this system, we conducted a comprehensive, multi\u2010parametric analysis comparing the effects of three commercially available dry diets varying in protein and fat content and live chironomid larvae (bloodworms), administered either via high\u2010frequency automated feeding or manual twice\u2010daily feeding.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A continuous diet of 24\u201036 feedings per day increased fish growth in weight but not in length compared to a standard twice\u2010daily regimen. Unexpectedly, while male lifespan remained unaffected by feeding regimen or diet, females subjected to high\u2010frequency automated feeding showed reduced longevity, particularly with high\u2010calorie diets. Additionally, pooled reproductive profiles suggested that reproductive output was associated with dietary fat and protein content as well as feeding frequency. Greater delivered food availability, particularly under high\u2010frequency feeding with energy\u2010rich diets, was associated with increased egg production, reduced embryo survival, and a shortened reproductive period.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      Together, these findings provide important insights for optimizing husbandry and experimental design in\n                      <jats:italic>N. furzeri,<\/jats:italic>\n                      supporting its broader application as a high\u2010throughput vertebrate model.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fragi.2026.1869084","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T04:36:50Z","timestamp":1789360610000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Sex-specific effects of automated feeding on lifespan and reproduction in Nothobranchius furzeri"],"prefix":"10.3389","volume":"7","author":[{"given":"Luca","family":"Dolfi","sequence":"first","affiliation":[{"name":"Center for Anatomy and Cell Biology, Medical University of Vienna","place":["Vienna, Austria"]},{"name":"BIO@SNS Laboratory, Scuola Normale Superiore of Pisa","place":["Pisa, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Kothmayer","sequence":"additional","affiliation":[{"name":"Center for Anatomy and Cell Biology, Medical University of Vienna","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elmar Erwin","family":"Ebner","sequence":"additional","affiliation":[{"name":"Center for Anatomy and Cell Biology, Medical University of Vienna","place":["Vienna, Austria"]},{"name":"Department of Biological Sciences and Pathobiology, Institute for Infectiology and Virology, University of Veterinary Medicine Vienna","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renate","family":"L\u00f6hnert","sequence":"additional","affiliation":[{"name":"Center for Anatomy and Cell Biology, Medical University of Vienna","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Pusch","sequence":"additional","affiliation":[{"name":"Center for Anatomy and Cell Biology, Medical University of Vienna","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,14]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0278302","article-title":"The effects of intensive feeding on reproductive performance in laboratory zebrafish (Danio rerio)","volume":"17","author":"Adatto","year":"2022","journal-title":"PLoS One"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1003","DOI":"10.1016\/j.exger.2013.04.011","article-title":"Fasting or caloric restriction for healthy aging","volume":"48","author":"Anton","year":"2013","journal-title":"Exp. 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