{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T07:00:49Z","timestamp":1785308449376,"version":"3.55.0"},"reference-count":58,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000272","name":"National Institute for Health and Care Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiomes"],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>The maternal microbiome plays a crucial role in pregnancy with growing evidence supporting vertical microbial transmission from mother to fetus. The placenta, once considered sterile, may serve as a conduit for this transfer. We hypothesized that the placental microbial signatures would be distinctly different from oral, fecal, or vaginal microbiomes in pregnant mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>To test this hypothesis, the obese BPH\/5 mouse (n=15), which spontaneously develops a preeclampsia (PE)-like phenotype, was compared to normotensive C57 (n=8) pregnant mice. 16S rRNA gene sequencing and bioinformatic analyses were conducted to assess microbial diversity and composition from samples collected at embryonic day 18.5 (feces, oral cavity, vagina, and placenta).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Alpha diversity analysis revealed that oral microbiomes of both BPH\/5 and C57 were significantly less diverse compared to the placental microbial signatures (p = 0.019 and &amp;lt;0.001, respectively). Beta diversity analysis confirmed distinct microbial communities across body sites and between strains (p &amp;lt; 0.001), while no significant differences were detected in placental and vaginal microbiomes (p &amp;gt; 0.05). Microbial composition analysis showed site-specific variations at the phylum and genus levels with Firmicutes and Bacteroidetes being dominant across all sites. BPH\/5 placentas were enriched in\n                      <jats:italic>Alistipes<\/jats:italic>\n                      ,\n                      <jats:italic>Lachnospiraceae_NK4A136<\/jats:italic>\n                      and\n                      <jats:italic>Helicobacter<\/jats:italic>\n                      . In contrast, C57 placentas were enriched in\n                      <jats:italic>Alistipes<\/jats:italic>\n                      ,\n                      <jats:italic>Lachnospiraceae_NK4A136<\/jats:italic>\n                      , and\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      suggesting strain-specific microbial alterations with common genera between maternal oral, fecal, vaginal, and placental communities.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings demonstrate that the placental microbial signatures are unique in a PE-like mouse model. Further studies are needed to elucidate the functional impact of these microbial differences on maternal and fetal PE outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/frmbi.2026.1881824","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T06:03:46Z","timestamp":1785305026000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Placental and maternal microbiome adaptations in a preeclamptic-like mouse model"],"prefix":"10.3389","volume":"5","author":[{"given":"Kalie F.","family":"Beckers","sequence":"first","affiliation":[{"name":"Division of Veterinary Medicine, Tulane National Primate Research Center, Tulane University","place":["Covington, LA, United States"]},{"name":"Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University","place":["Baton Rouge, LA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Schulz","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Southeastern Louisiana University","place":["Hammond, LA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliet P.","family":"Flanagan","sequence":"additional","affiliation":[{"name":"Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University","place":["Baton Rouge, LA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin-Chi","family":"Liu","sequence":"additional","affiliation":[{"name":"Office of Research and Graduate Education, School of Veterinary Medicine, Louisiana State University","place":["Baton Rouge, LA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary W.","family":"Childers","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Southeastern Louisiana University","place":["Hammond, LA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella N.","family":"Faulkner","sequence":"additional","affiliation":[{"name":"Clinical Sciences, Colorado State University College of Veterinary Medicine and Biomedical Sciences","place":["Fort Collins, CO, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny L.","family":"Sones","sequence":"additional","affiliation":[{"name":"Clinical Sciences, Colorado State University College of Veterinary Medicine and Biomedical Sciences","place":["Fort Collins, CO, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"237ra65","DOI":"10.1126\/scitranslmed.3008599","article-title":"The placenta harbors a unique microbiome","volume":"6","author":"Aagaard","year":"2014","journal-title":"Sci. Transl. Med."},{"key":"B2","doi-asserted-by":"publisher","first-page":"662","DOI":"10.1111\/jog.12619","article-title":"Microbiome of the placenta in pre-eclampsia supports the role of bacteria in the multifactorial cause of pre-eclampsia","volume":"41","author":"Amarasekara","year":"2015","journal-title":"J. Obstet Gynaecol Res."},{"key":"B3","volume-title":"Permutational multivariate analysis of variance","author":"Anderson","year":"2005"},{"key":"B4","doi-asserted-by":"publisher","first-page":"670","DOI":"10.1902\/jop.2007.060362","article-title":"Evidence of periopathogenic microorganisms in placentas of women with preeclampsia","volume":"78","author":"Barak","year":"2007","journal-title":"J. Periodontol"},{"key":"B5","doi-asserted-by":"publisher","first-page":"F334","DOI":"10.1136\/archdischild-2012-303035","article-title":"The individual-specific and diverse nature of the preterm infant microbiota","volume":"98","author":"Barrett","year":"2013","journal-title":"Arch. Dis. Childhood-Fetal Neonatal Edition"},{"key":"B6","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1016\/s1470-0328(02)01349-6","article-title":"Possible association between amniotic fluid micro-organism infection and microflora in the mouth","volume":"109","author":"Bearfield","year":"2002","journal-title":"BJOG: An Int. J. Obstet Gynaecol"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.3390\/ani13121999","article-title":"Metagenetic analysis of the pregnant microbiome in horses","volume":"13","author":"Beckers","year":"2023","journal-title":"Animals"},{"key":"B8","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1152\/physiolgenomics.00106.2024","article-title":"Pregnancy-specific shifts in the maternal microbiome and metabolome in the BPH\/5 mouse model of superimposed preeclampsia","volume":"57","author":"Beckers","year":"2025","journal-title":"Physiol. Genomics"},{"key":"B9","doi-asserted-by":"publisher","first-page":"H1","DOI":"10.1152\/ajpheart.00469.2019","article-title":"Maternal microbiome and the hypertensive disorder of pregnancy, preeclampsia","volume":"318","author":"Beckers","year":"2020","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1016\/j.chom.2023.01.018","article-title":"Mother-to-infant microbiota transmission and infant microbiota development across multiple body sites","volume":"31","author":"Bogaert","year":"2023","journal-title":"Cell. Host Microbe"},{"key":"B11","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1016\/s0029-7844(02)02314-1","article-title":"Maternal periodontal disease is associated with an increased risk for preeclampsia","volume":"101","author":"Boggess","year":"2003","journal-title":"Obstet Gynecol"},{"key":"B12","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: high-resolution sample inference from Illumina amplicon data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat. Methods"},{"key":"B13","doi-asserted-by":"publisher","first-page":"551","DOI":"10.2147\/ijwh.s142730","article-title":"The oral microbiome and adverse pregnancy outcomes","volume":"9","author":"Cobb","year":"2017","journal-title":"Int. J. Women\u2019s Health"},{"key":"B14","doi-asserted-by":"publisher","first-page":"4356","DOI":"10.1128\/iai.62.10.4356-4361.1994","article-title":"Effects of a Porphyromonas gingivalis infection on inflammatory mediator response and pregnancy outcome in hamsters","volume":"62","author":"Collins","year":"1994","journal-title":"Infect. Immun."},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.3389\/fbrio.2023.1322165","article-title":"Oral origin of the placenta microbiome in pregnant women with preeclampsia","volume":"2","author":"Cooper","year":"2024","journal-title":"Front. Bacteriol"},{"key":"B16","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1161\/hy02t2.102904","article-title":"Discovery of a spontaneous genetic mouse model of preeclampsia","volume":"39","author":"Davisson","year":"2002","journal-title":"Hypertension"},{"key":"B17","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1097\/nnr.0000000000000133","article-title":"The maternal microbiome and pregnancy outcomes that impact infant health: a review","volume":"15","author":"Dunlop","year":"2015","journal-title":"Adv. Neonatal Care: Off. J. Natl. Assoc. Neonatal Nurses"},{"key":"B18","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1097\/nmc.0000000000000372","article-title":"The maternal gut microbiome during pregnancy","volume":"42","author":"Edwards","year":"2017","journal-title":"MCN Am. J. Maternal Child Nurs."},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00213.2025","article-title":"Monitored food intake in early pregnancy modulates the maternal microbiome in the obese BPH\/5 mouse model of superimposed preeclampsia","volume":"58","author":"Fan","year":"2026","journal-title":"Physiol. Genomics"},{"key":"B20","doi-asserted-by":"publisher","first-page":"1789","DOI":"10.1128\/iai.01395-09","article-title":"Transmission of diverse oral bacteria to murine placenta: evidence for the oral microbiome as a potential source of intrauterine infection","volume":"78","author":"Fardini","year":"2010","journal-title":"Infect. Immun."},{"key":"B21","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/j.chom.2018.06.005","article-title":"Mother-to-infant microbial transmission from different body sites shapes the developing infant gut microbiome","volume":"24","author":"Ferretti","year":"2018","journal-title":"Cell. Host Microbe"},{"key":"B22","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1038\/s41522-025-00720-y","article-title":"Mother-to-infant vertical transmission in early life: a systematic review and proportional meta-analysis of Bifidobacterium strain transmissibility","volume":"11","author":"Flores Ventura","year":"2025","journal-title":"NPJ Biofilms Microbiomes"},{"key":"B23","first-page":"63","article-title":"Leaky gut syndromes: breaking the vicious cycle","volume":"145","author":"Galland","year":"1995","journal-title":"Townsend Letter For. Doctors"},{"key":"B24","doi-asserted-by":"publisher","first-page":"1129","DOI":"10.4049\/jimmunol.1402220","article-title":"Prevention of defective placentation and pregnancy loss by blocking innate immune pathways in a syngeneic model of placental insufficiency","volume":"195","author":"Gelber","year":"2015","journal-title":"J. Immunol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1097\/01.aoa.0000344666.82463.8d","article-title":"Epidemiology and causes of preterm birth","volume":"371","author":"Goldenberg","year":"2008","journal-title":"Lancet"},{"key":"B26","doi-asserted-by":"publisher","first-page":"2860","DOI":"10.1038\/s41598-017-03066-4","article-title":"Contributions of the maternal oral and gut microbiome to placental microbial colonization in overweight and obese pregnant women","volume":"7","author":"Gomez-Arango","year":"2017","journal-title":"Sci. Rep."},{"key":"B27","doi-asserted-by":"publisher","first-page":"2272","DOI":"10.1128\/iai.72.4.2272-2279.2004","article-title":"Fusobacterium nucleatum induces premature and term stillbirths in pregnant mice: implication of oral bacteria in preterm birth","volume":"72","author":"Han","year":"2004","journal-title":"Infect. Immun."},{"key":"B28","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1093\/biolre\/ioac067","article-title":"Comparing the maternal-fetal microbiome of humans and cattle: a translational assessment of the reproductive, placental, and fetal gut microbiomes","volume":"107","author":"Hummel","year":"2022","journal-title":"Biol. Reprod."},{"key":"B29","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1177\/1933719113488838","article-title":"Diversity of the vaginal microbiome correlates with preterm birth","volume":"21","author":"Hyman","year":"2014","journal-title":"Reprod. Sci."},{"key":"B30","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/j.resmic.2007.12.007","article-title":"Is meconium from healthy newborns actually sterile","volume":"159","author":"Jim\u00e9nez","year":"2008","journal-title":"Res. Microbiol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1093\/infdis\/164.1.94","article-title":"Control of the microbial flora of the vagina by H2O2-generating lactobacilli","volume":"164","author":"Klebanoff","year":"1991","journal-title":"J. Infect. Dis."},{"key":"B32","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1038\/nmeth.1650","article-title":"Bayesian community-wide culture-independent microbial source tracking","volume":"8","author":"Knights","year":"2011","journal-title":"Nat. Methods"},{"key":"B33","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1016\/j.cell.2012.07.008","article-title":"Host remodeling of the gut microbiome and metabolic changes during pregnancy","volume":"150","author":"Koren","year":"2012","journal-title":"Cell."},{"key":"B34","doi-asserted-by":"publisher","first-page":"5112","DOI":"10.1128\/aem.01043-13","article-title":"Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform","volume":"79","author":"Kozich","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1186\/s40168-016-0172-3","article-title":"Comparison of placenta samples with contamination controls does not provide evidence for a distinct placenta microbiota","volume":"4","author":"Lauder","year":"2016","journal-title":"Microbiome"},{"key":"B36","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1186\/s40168-018-0575-4","article-title":"Lack of detection of a human placenta microbiome in samples from preterm and term deliveries","volume":"6","author":"Leiby","year":"2018","journal-title":"Microbiome"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00417","article-title":"The microbiota and epigenetic regulation of T helper 17\/regulatory T cells: in search of a balanced immune system","volume":"8","author":"Luo","year":"2017","journal-title":"Front. Immunol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1097\/01.aoa.0000344694.90980.89","article-title":"Alternative complement pathway activation fragment Bb in early pregnancy as a predictor of preeclampsia","volume":"198","author":"Lynch","year":"2008","journal-title":"Am. J. Obstet Gynecol"},{"key":"B39","doi-asserted-by":"publisher","first-page":"2133","DOI":"10.1902\/jop.2005.76.11-s.2133","article-title":"Effects of maternal Campylobacter rectus infection on murine placenta, fetal and neonatal survival, and brain development","volume":"76","author":"Offenbacher","year":"2005","journal-title":"J. Periodontol"},{"key":"B40","first-page":"1","article-title":"Package \u2018vegan\u2019","volume":"2","author":"Oksanen","year":"2013","journal-title":"Community Ecol. Package Version"},{"key":"B41","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1152\/physiolgenomics.00102.2018","article-title":"Obesity \u201ccomplements\u201d preeclampsia","volume":"51","author":"Olson","year":"2019","journal-title":"Physiol. Genomics"},{"key":"B42","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.placenta.2016.12.003","article-title":"Maternal health and the placental microbiome","volume":"54","author":"Pelzer","year":"2017","journal-title":"Placenta"},{"key":"B43","doi-asserted-by":"publisher","first-page":"e724","DOI":"10.1542\/peds.2006-1649","article-title":"Bacterial imprinting of the neonatal immune system: lessons from maternal cells","volume":"119","author":"Perez","year":"2007","journal-title":"Pediatrics"},{"key":"B44","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1186\/s40168-017-0268-4","article-title":"A critical assessment of the \u201csterile womb\u201d and \u201cin utero colonization\u201d hypotheses: implications for research on the pioneer infant microbiome","volume":"5","author":"Perez-Mu\u00f1oz","year":"2017","journal-title":"Microbiome"},{"key":"B45","doi-asserted-by":"publisher","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: improved data processing and web-based tools","volume":"41","author":"Quast","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"B46","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1007\/s11906-017-0784-4","article-title":"The complement system and preeclampsia","volume":"19","author":"Regal","year":"2017","journal-title":"Curr. Hypertension Rep."},{"key":"B47","doi-asserted-by":"publisher","first-page":"R49","DOI":"10.1152\/ajpregu.00334.2018","article-title":"Dyslipidemia and the role of the adipose tissue in early pregnancy in the BPH\/5 mouse model for preeclampsia","volume":"317","author":"Reijnders","year":"2019","journal-title":"Am. J. Physiol. Regul. Intgr Comp. Physiol"},{"key":"B48","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1172\/jci.insight.75351","article-title":"Decidual Cox2 inhibition improves fetal and maternal outcomes in a preeclampsia-like mouse model","volume":"1","author":"Sones","year":"2016","journal-title":"JCI Insight"},{"key":"B49","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1016\/j.ajog.2017.05.030","article-title":"Early pregnancy vaginal microbiome trends and preterm birth","volume":"217","author":"Stout","year":"2017","journal-title":"Am. J. Obstet Gynecol"},{"key":"B50","doi-asserted-by":"publisher","first-page":"R485","DOI":"10.1152\/ajpregu.00512.2016","article-title":"Adverse metabolic phenotype of female offspring exposed to preeclampsia in utero: a characterization of the BPH\/5 mouse in postnatal life","volume":"312","author":"Sutton","year":"2017","journal-title":"Am. J. Physiol. Regul. Intgr Comp. Physiol."},{"key":"B51","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1186\/s12967-018-1482-y","article-title":"Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation","volume":"16","author":"Tersigni","year":"2018","journal-title":"J. Transl. Med."},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.1602","article-title":"Characterisation of the human uterine microbiome in non-pregnant women through deep sequencing of the V1-2 region of the 16S rRNA gene","volume":"4","author":"Verstraelen","year":"2016","journal-title":"PeerJ"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00140-17","article-title":"Development of the human mycobiome over the first month of life and across body sites","volume":"3","author":"Ward","year":"2018","journal-title":"mSystems"},{"key":"B54","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1111\/ajo.12754","article-title":"A retrospective pilot study to determine whether the reproductive tract microbiota differs between women with a history of infertility and fertile women","volume":"58","author":"Wee","year":"2018","journal-title":"Aust. New Z. J. Obstet Gynaecol"},{"key":"B55","doi-asserted-by":"publisher","first-page":"7025","DOI":"10.1080\/14767058.2021.1936487","article-title":"Investigating the origin of the fetal gut and placenta microbiome in twins","volume":"35","author":"Williams","year":"2022","journal-title":"J. Maternal-Fetal Neonatal Med."},{"key":"B56","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1111\/1471-0528.13115","article-title":"The vaginal microbiome, vaginal anti-microbial defence mechanisms and the clinical challenge of reducing infection-related preterm birth","volume":"122","author":"Witkin","year":"2015","journal-title":"BJOG: An Int. J. Obstet Gynaecol"},{"key":"B57","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1136\/gutjnl-2022-328970","article-title":"Microbial transmission, colonisation and succession: from pregnancy to infancy","volume":"72","author":"Xiao","year":"2023","journal-title":"Gut"},{"key":"B58","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1038\/nrmicro3330","article-title":"Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences","volume":"12","author":"Yarza","year":"2014","journal-title":"Nat. Rev. Microbiol."}],"container-title":["Frontiers in Microbiomes"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/frmbi.2026.1881824\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T06:03:47Z","timestamp":1785305027000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/frmbi.2026.1881824\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,29]]},"references-count":58,"alternative-id":["10.3389\/frmbi.2026.1881824"],"URL":"https:\/\/doi.org\/10.3389\/frmbi.2026.1881824","relation":{},"ISSN":["2813-4338"],"issn-type":[{"value":"2813-4338","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,29]]},"article-number":"1881824"}}