{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:16:01Z","timestamp":1781846161526,"version":"3.54.5"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T00:00:00Z","timestamp":1720742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Oral. Health"],"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To test the biological properties of a novel non-restorative treatment method for arresting dentin caries based on silver nanoclusters (AgNCls) synthesized in polymethacrylic acid (PMAA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Synthesis of AgNCls was performed by photoreduction of AgNO<jats:sub>3<\/jats:sub> in PMAA with 355\u2005nm\/wavelength light. AgNCls\/PMAA was characterized by absorption\/fluorescence spectroscopy and optical and atomic force microscopy. The stability of the clusters in an aerated PMAA solution was evaluated by means of fluorescence spectroscopy. Cytotoxicity was assessed using the MTT assay and antibacterial effect was determined for minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and colony forming (CFU) of <jats:italic>Streptococcus mutans<\/jats:italic> (<jats:italic>S. mutans<\/jats:italic>) and <jats:italic>Lactobacillus acidophilus<\/jats:italic> (<jats:italic>L. acidophilus<\/jats:italic>). 38% Silver Diammine Fluoride (SDF) was used for the control groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Chemical and structural identity of the clusters did not change within 9 months; Cell viability of 92%\u201389% was found after 24\u201348\u2005h respectively. MIC and MBC were determined from 1:16 and 1:8 dilutions, respectively. Log CFU counts of <jats:italic>S. mutans,<\/jats:italic> and <jats:italic>L. acidophilus<\/jats:italic> treated with AgNCls\/PMAA (3.4\u2005ppm of silver) were significantly lower than in the control groups and even lower than when the same bacterial strains were treated with SDF (15,525\u2005ppm of silver).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>AgNCls\/PMAA presented chemical stability, acceptable cytotoxicity, and a potential antibacterial effect for strains associated with caries lesions at very low concentrations of silver.<\/jats:p><\/jats:sec>","DOI":"10.3389\/froh.2024.1408181","type":"journal-article","created":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T06:01:03Z","timestamp":1720764063000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Biological properties of a novel solution based on silver nanoclusters for arresting dentin caries"],"prefix":"10.3389","volume":"5","author":[{"given":"Gustavo Fabi\u00e1n","family":"Molina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Bel\u00e9n","family":"Cabal\u00e9n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Pablo","family":"Aranguren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Ariel","family":"Pino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael Francis","family":"Burrow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,7,12]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"00948","DOI":"10.1016\/j.identj.2023.10.013","article-title":"Silver compounds for caries management","author":"Xu","year":"2023","journal-title":"Int Dent J"},{"key":"B2","doi-asserted-by":"publisher","first-page":"809","DOI":"10.1016\/j.jdent.2013.06.009","article-title":"Inhibitory effect of silver diamine fluoride on dentine demineralisation and collagen degradation","volume":"41","author":"Mei","year":"2013","journal-title":"J Dent"},{"key":"B3","doi-asserted-by":"publisher","first-page":"e20220306","DOI":"10.1590\/1678-7757-2022-0306","article-title":"Evaluation of the remineralizing capacity of silver diamine fluoride on demineralized dentin under pH-cycling conditions","volume":"31","author":"Cifuentes-Jim\u00e9nez","year":"2023","journal-title":"J Appl Oral Sci"},{"key":"B4","doi-asserted-by":"publisher","first-page":"931327","DOI":"10.3389\/fbioe.2022.931327","article-title":"The anticariogenic efficacy of nano silver fluoride","volume":"10","author":"Pushpalatha","year":"2022","journal-title":"Front Bioeng Biotechnol"},{"key":"B5","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1111\/adj.12985","article-title":"The effect of silver nanoparticles in addition to sodium fluoride on remineralization of artificial root dentin caries","volume":"69","author":"Kongyodsueb","year":"2024","journal-title":"Aust Dent J"},{"key":"B6","doi-asserted-by":"publisher","first-page":"S13","DOI":"10.5005\/jp-journals-10005-2575","article-title":"A comparison of antimicrobial efficacy of silver-based preventive restorations (silver nitrate, silver diamine fluoride, and silver nanoparticles) against Streptococcus mutans monospecies biofilm model","volume":"16","author":"Sharma","year":"2023","journal-title":"Int J Clin Pediatr Dent"},{"key":"B7","doi-asserted-by":"publisher","first-page":"21126","DOI":"10.1038\/s41598-023-48519-1","article-title":"Application of 20% silver nanoclusters in polymethacrylic acid on simulated dentin caries; 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