{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T04:30:57Z","timestamp":1786336257094,"version":"build-2736575974"},"reference-count":56,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Oral Health"],"abstract":"\n Objective<\/jats:title>\n \n Dihydromyricetin has inhibitory effects on various pathogens, but its effect on\n Porphyromonas gingivalis<\/jats:italic>\n is not yet known. This study investigates the effects of dihydromyricetin on planktonic cells, biofilms, and related virulence factors of\n Porphyromonas gingivalis<\/jats:italic>\n , evaluates the\n in vitro<\/jats:italic>\n antibacterial activity of dihydromyricetin against\n Porphyromonas gingivalis<\/jats:italic>\n , and preliminarily explores its possible antibacterial mechanisms.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The microdilution method was used to explore the inhibitory effect of dihydromyricetin on planktonic\n Porphyromonas gingivalis<\/jats:italic>\n . The impact of dihydromyricetin on biofilm formation was assessed via crystal violet staining. The effects on the expression of fimbrial genes (\n fimA<\/jats:italic>\n ,\n fimR<\/jats:italic>\n ,\n fimS<\/jats:italic>\n ) and the capsular polysaccharide gene\n PGN-1525<\/jats:italic>\n were examined using qRT-PCR. Protein substrate assays were conducted to evaluate the influence of dihydromyricetin on the proteolytic activity of gingipains (arginine protease and lysine protease).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n 1% DMSO has no significant effect on the growth of\n Porphyromonas gingivalis<\/jats:italic>\n . MIC of dihydromyricetin against planktonic\n Porphyromonas gingivalis<\/jats:italic>\n was 160\u2005\u00b5g\/mL, while the MBC was 320\u2005\u00b5g\/mL. DMY at 160\u2013640\u2005\u00b5g\/mL downregulated fimbrial (\n fimA<\/jats:italic>\n ,\n fimR<\/jats:italic>\n ,\n fimS<\/jats:italic>\n ) and capsular polysaccharide (\n PGN_1525<\/jats:italic>\n ) gene expression; 40\u2013160\u2005\u00b5g\/mL DMY concentration-dependently suppressed Rgp and Kgp proteolytic activity.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Dihydromyricetin has shown relatively significant antibacterial activity and virulence factor inhibition against\n Porphyromonas gingivalis<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/froh.2026.1872637","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T04:22:02Z","timestamp":1786335722000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Dihydromyricetin exhibits potent antibacterial activity and virulence factor inhibitory effects against Porphyromonas gingivalis"],"prefix":"10.3389","volume":"7","author":[{"given":"Yanhui","family":"Peng","sequence":"first","affiliation":[{"name":"Hebei Key Laboratory of Stomatology\/Hebei Technology Innovation Center of Oral Health, School and Hospital of Stomatology, Hebei Medical University","place":["Shijiazhuang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Liu","sequence":"additional","affiliation":[{"name":"Hebei Key Laboratory of Stomatology\/Hebei Technology Innovation Center of Oral Health, School and Hospital of Stomatology, Hebei Medical University","place":["Shijiazhuang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Liu","sequence":"additional","affiliation":[{"name":"Hebei Key Laboratory of Stomatology\/Hebei Technology Innovation Center of Oral Health, School and Hospital of Stomatology, Hebei Medical University","place":["Shijiazhuang, 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