{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:58:38Z","timestamp":1790315918327,"version":"4.1.0"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T00:00:00Z","timestamp":1732665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Reprod. Health"],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Depletion of <jats:italic>Lactobacillus<\/jats:italic> species and an overgrowth of anaerobes in the vaginal tract bacterial vaginosis (BV)], is associated with non-optimal reproductive health outcomes, and increased susceptibility to sexually transmitted infections (STIs). BV is currently treated with antibiotics, although these provide suboptimal cure levels and high recurrence rates. Vaginal microbiota transplantation (VMT), the transfer of vaginal fluid from healthy donors with an optimal vaginal microbiota to a recipient with BV, has been proposed as an alternative treatment strategy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Here, we investigated knowledge and perceptions of blood donors to the concept of an optimal vaginal microbiome and VMT via the Western Cape Blood Service (WCBS) clinics in Cape Town, South Africa, by a self-administered questionnaire.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results &amp;amp; discussion<\/jats:title><jats:p>Analysis of responses from 106 eligible women showed that 86% (91\/106) would consider donating samples. Responses significantly associated with willingness to donate vaginal samples included: (1) belief that helping others outweighs the inconvenience of donating vaginal sample (<jats:italic>p<\/jats:italic> = 1.093e<jats:sup>\u221205<\/jats:sup>) and (2) prior knowledge of the concept of a healthy vaginal microbiome (<jats:italic>p<\/jats:italic>\u2009=\u20090.001). Most potential donors (59\/91; 65%) were willing to receive a VMT themselves if needed. Participants who were unwilling to donate vaginal samples (15\/106; 14%) indicated that vaginal sample collection would be unpleasant and\/or embarrassing. The benefits of a collaboration with WCBS for this project include the naturally altruistic nature of blood donors, the constant in-flow of donors to WCBS clinics, and the infrastructure and logistical aspects in place. Data from this observational study highlight factors affecting the willingness of blood donors to become vaginal sample donors.<\/jats:p><\/jats:sec>","DOI":"10.3389\/frph.2024.1446809","type":"journal-article","created":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T06:39:25Z","timestamp":1732689565000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Knowledge and perceptions of blood donors of the Western Cape Blood Services, South Africa, toward vaginal sample donation for biobanking"],"prefix":"10.3389","volume":"6","author":[{"given":"Shantelle","family":"Claassen-Weitz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Kullin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elloise","family":"du 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