{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T06:10:06Z","timestamp":1756793406051,"version":"3.44.0"},"reference-count":19,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Transplant."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Our objective was to model Ischemia-Reperfusion (IR) injuries by <jats:italic>ex-vivo<\/jats:italic> perfusion of porcine lungs with whole blood containing the inflammatory cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Lungs and whole blood were collected from 12 pigs and submitted to cold ischemia time (CIT) of 1 or 18\u2005h. The lungs were then ventilated and perfused for 6\u2005h at 37\u00b0C using donor whole blood. Pulmonary pressure was 20\u2005mmHg.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared to the short CIT group, the long CIT group had a lower maximum perfusion flow rate (mean difference in % cardiac output, \u221239%; 95% CI, \u221266 to \u221212; <jats:italic>P<\/jats:italic>\u2009=\u20090.005) and higher pulmonary vascular resistance (mean difference, 1,077 dyne\u00b7s\u00b7cm<jats:sup>\u2212<\/jats:sup>\u2075; 95% CI, 685\u20131,469; <jats:italic>P<\/jats:italic>\u2009&amp;lt;\u20090.001). Neutrophils decreased more in the long CIT group (mean difference, \u2212744.02\u2005cells\/mm<jats:sup>3<\/jats:sup>; 95% CI, \u22121,343.11 to \u2212144.92; <jats:italic>P<\/jats:italic>\u2009=\u20090.017), suggesting sequestration in the lung parenchyma. Interleukin-6 and \u22128 levels after 6\u2005h were significantly higher in the long CIT group (mean differences, 1.1\u2005pg\/ml; 95% CI, 0.39\u20131.8; <jats:italic>P<\/jats:italic>\u2009=\u20090.003; and 29.31\u2005pg\/ml; 95%CI, 16.00\u201342.61; <jats:italic>P<\/jats:italic>\u2009&amp;lt;\u20090.001; respectively). Progressive microvasculopathy resulting in lymphangiectasia and peribronchovascular inflammatory infiltrates were seen in both groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>After 18\u2005h of CIT, <jats:italic>ex-vivo<\/jats:italic> whole-blood perfusion for 6\u2005h replicated features of IR injuries.<\/jats:p><\/jats:sec>","DOI":"10.3389\/frtra.2025.1651671","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T05:34:55Z","timestamp":1756791295000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Ischemia-reperfusion injury with a model of porcine whole-blood ex-vivo lung perfusion"],"prefix":"10.3389","volume":"4","author":[{"given":"Jean-Baptiste","family":"Menager","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Mercier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin","family":"Issard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria-Rosa","family":"Ghigna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanne","family":"Tran Van Nhieu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benoit","family":"Decante","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julien","family":"Guihaire","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elie","family":"Fadel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabrice","family":"Antigny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olaf","family":"Mercier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,9,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"600","DOI":"10.1007\/s00068-007-7152-z","article-title":"Ischemia-reperfusion injury","volume":"33","author":"Dorweiler","year":"2007","journal-title":"Eur J Trauma Emerg Surg"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1454","DOI":"10.1016\/j.healun.2004.11.049","article-title":"Report of the ISHLT working group on primary lung graft dysfunction part II: definition. 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