{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T06:28:11Z","timestamp":1787120891326,"version":"build-2736575974"},"reference-count":42,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Transplant."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      During\n                      <jats:italic>ex vivo<\/jats:italic>\n                      lung perfusion (EVLP), perfusate pH, electrolyte, and glucose concentrations often deviate from physiological ranges. We evaluated the effect of a corrective solution on maintaining these parameters within physiological limits during 12\u2005h of EVLP in a porcine model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Twelve porcine lung blocks were harvested and cold-preserved before undergoing 12\u2005h of normothermic EVLP. Lung blocks were randomly assigned to two groups based on the perfusate replacement strategy. All lungs were perfused with Steen solution, mean priming volume was 1,525\u2009\u00b1\u2009340 and 1,325\u2009\u00b1\u2009232\u2005mL, with 100\u2005mL (5%\u20139%) replaced every two hours by either fresh Steen solution (standard group,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20096) or a modified solution designed to restore physiological composition (corrected group,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20096). Perfusate electrolytes, glucose, cytokines, pH, partial oxygen pressure (PO\u2082), pulmonary vascular resistance (PVR), and compliance were measured. After EVLP, left lungs were transplanted during 4\u2005h into donor-related pigs, while right lungs were analyzed by histology and electron microscopy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Perfusate correction successfully maintained electrolyte, pH, and glucose levels within target ranges during EVLP. The corrected group demonstrated higher lung compliance and oxygenation, reduced edema, more hyaline membranes, and a greater density of Kohn's pores after EVLP. PVR was similar between groups, and no significant difference in\n                      <jats:italic>\u0394<\/jats:italic>\n                      PO\u2082 was observed post-transplantation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Maintaining physiological perfusate composition improved lung function during 12-hour EVLP in swine; however, no post-transplant benefit was observed. Further studies are warranted to assess the impact of this strategy during prolonged EVLP and to compare it with alternative approaches.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/frtra.2026.1899960","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T05:48:59Z","timestamp":1787118539000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Prolonged ex vivo lung perfusion with corrected perfusate: functional and structural benefits in swine"],"prefix":"10.3389","volume":"5","author":[{"given":"Justin","family":"Issard","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery and Heart-Lung Transplantation, Marie Lannelongue Hospital, Universit\u00e9 Paris-Saclay","place":["Le Plessis Robinson, France"]},{"name":"Hypertension Pulmonaire: Physiopathologie et Innovation Th\u00e9rapeutique (HPPIT), H\u00f4pital Bic\u00eatre (AP-HP), H\u00f4pital Marie 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H\u00f4pital Marie Lannelongue, FHU Andr\u00e9 Cournand, ERN-LUNG, Universit\u00e9 Paris-Saclay, INSERM UMR_S 1358","place":["Le Plessis Robinson, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Fadel","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery and Heart-Lung Transplantation, Marie Lannelongue Hospital, Universit\u00e9 Paris-Saclay","place":["Le Plessis Robinson, France"]},{"name":"Hypertension Pulmonaire: Physiopathologie et Innovation Th\u00e9rapeutique (HPPIT), H\u00f4pital Bic\u00eatre (AP-HP), H\u00f4pital Marie Lannelongue, FHU Andr\u00e9 Cournand, ERN-LUNG, Universit\u00e9 Paris-Saclay, INSERM UMR_S 1358","place":["Le Plessis Robinson, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuya","family":"Nobori","sequence":"additional","affiliation":[{"name":"Hypertension Pulmonaire: Physiopathologie et Innovation Th\u00e9rapeutique (HPPIT), H\u00f4pital Bic\u00eatre (AP-HP), H\u00f4pital Marie Lannelongue, FHU Andr\u00e9 Cournand, ERN-LUNG, Universit\u00e9 Paris-Saclay, INSERM UMR_S 1358","place":["Le Plessis Robinson, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melwan","family":"Izem","sequence":"additional","affiliation":[{"name":"Hypertension Pulmonaire: Physiopathologie et Innovation Th\u00e9rapeutique (HPPIT), H\u00f4pital Bic\u00eatre (AP-HP), H\u00f4pital Marie Lannelongue, FHU Andr\u00e9 Cournand, ERN-LUNG, Universit\u00e9 Paris-Saclay, INSERM UMR_S 1358","place":["Le Plessis Robinson, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria-Rosa","family":"Ghigna","sequence":"additional","affiliation":[{"name":"Department of Pathology, Marie Lannelongue Hospital, Groupe Hospitalier Paris-Saint-Joseph","place":["Le Plessis Robinson, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elie","family":"Fadel","sequence":"additional","affiliation":[{"name":"Department of Thoracic 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