{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:34:41Z","timestamp":1753893281944,"version":"3.41.2"},"reference-count":53,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Surg."],"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Chronic osteomyelitis, including implant-related prosthetic joint infection, is extremely difficult to cure. We develop vancomycin containing release systems from poly(<jats:sc>d<\/jats:sc>,<jats:sc>l<\/jats:sc>-lactide) (PDLLA) and poly(<jats:sc>d<\/jats:sc>,<jats:sc>l<\/jats:sc>-lactide-co-glycolide) (PLGA) composites with beta-tricalcium phosphate (\u03b2-TCP) to treat methicillin-resistant <jats:italic>Staphylococcus aureus<\/jats:italic> osteomyelitis. We ask whether vancomycin containing PDLLA\/\u03b2-TCP and PLGA\/\u03b2-TCP composites will prevent early biofilm formation, allow cell proliferation and osteogenic differentiation, and stimulate osteogenic signaling molecules in the absence of an osteogenic medium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Composites were produced and characterized with scanning electron microscopy. <jats:italic>In vitro<\/jats:italic> vancomycin release was assessed for 6 weeks. Biofilm prevention was calculated by crystal violet staining. Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) and osteosarcoma cell (SaOS-2) proliferation and differentiation were assessed with water soluble tetrazolium salt and alkaline phosphatase (ALP) staining. Real-time quantitative polymerase chain reaction defined osteogenic signaling molecules for hBM-MSCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Totally, 3.1\u2009\u00b1\u20090.2\u2005mg and 3.4\u2009\u00b1\u20090.4\u2005mg vancomycin released from PDLLA\/\u03b2-TCP and the PLGA\/\u03b2-TCP composites, respectively, and inhibited early biofilm formation. hBM-MSCs and SaOS-2 cells proliferated on the composites and stimulated ALP activity of cells. Runt-related transcription factor 2 (RUNX2) and SRY-Box transcription Factor 9 (SOX9) expressions were, however, lower with composites when compared with control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Vancomycin containing PDLLA\/\u03b2-TCP and PLGA\/\u03b2-TCP composites inhibited early biofilm formation and proliferated and differentiated hBM-MSCs and SaOS-2 cells, but osteogenesis-related RUNX2 and SOX9 transcription factors were not strongly expressed in the absence of an osteogenic medium for 14 days.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fsurg.2022.885241","type":"journal-article","created":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T05:58:43Z","timestamp":1656655123000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Vancomycin Containing PDLLA and PLGA\/\u03b2-TCP Inhibit Biofilm Formation but Do Not Stimulate Osteogenic Transformation of Human Mesenchymal Stem Cells"],"prefix":"10.3389","volume":"9","author":[{"given":"Berna","family":"Kankilic","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erdal","family":"Bayramli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petek","family":"Korkusuz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hakan","family":"Eroglu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Burcin","family":"Sener","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pelin","family":"Mutlu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feza","family":"Korkusuz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,7,1]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/jor.2016.10.004","article-title":"Osteomyelitis: recent advances in pathophysiology and therapeutic strategies","volume":"14","author":"Birt","year":"2017","journal-title":"J Orthop"},{"key":"B2","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.biomaterials.2015.12.012","article-title":"Biomaterials approaches to treating implant-associated osteomyelitis","volume":"81","author":"Inzana","year":"2016","journal-title":"Biomaterials"},{"key":"B3","doi-asserted-by":"publisher","first-page":"1216","DOI":"10.1016\/j.arth.2013.11.026","article-title":"Mortality following revision total knee arthroplasty: a matched cohort study of septic versus aseptic revisions","volume":"29","author":"Choi","year":"2014","journal-title":"J Arthroplasty"},{"key":"B4","doi-asserted-by":"publisher","first-page":"1492","DOI":"10.1016\/j.arth.2015.03.035","article-title":"Quantifying the burden of revision total joint arthroplasty for periprosthetic infection","volume":"30","author":"Kamath","year":"2015","journal-title":"J Arthroplasty"},{"key":"B5","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1007\/s12178-018-9500-5","article-title":"New innovations in the treatment of PJI and biofilms\u2014clinical and preclinical topics","volume":"11","author":"Taha","year":"2018","journal-title":"Curr Rev Musculoskelet Med"},{"key":"B6","doi-asserted-by":"publisher","first-page":"1073","DOI":"10.1016\/j.bioactmat.2020.09.031","article-title":"3D Additive manufactured composite scaffolds with antibiotic-loaded lamellar fillers for bone infection prevention and tissue regeneration","volume":"6","author":"Torres","year":"2021","journal-title":"Bioactive Mater"},{"key":"B7","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/j.msec.2013.12.038","article-title":"Preparation and characterization of antimicrobial nano-hydroxyapatite composites","volume":"37","author":"Yu","year":"2014","journal-title":"Mater Sci Eng C"},{"key":"B8","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.1007\/s10856-012-4585-7","article-title":"New PMMA-based composites for preparing spacer devices in prosthetic infections","volume":"23","author":"Giavaresi","year":"2012","journal-title":"J Mater Sci Mater Med"},{"key":"B9","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1002\/pat.729","article-title":"Polymers for tissue engineering, medical devices, and regenerative medicine. 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