{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T22:04:00Z","timestamp":1775945040203,"version":"3.50.1"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T00:00:00Z","timestamp":1664323200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007322","name":"Binzhou Medical University","doi-asserted-by":"publisher","award":["BY2012KJZD02"],"award-info":[{"award-number":["BY2012KJZD02"]}],"id":[{"id":"10.13039\/501100007322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Surg."],"abstract":"<jats:p>Anastomotic techniques are of vital importance in restoring gastrointestinal continuity after resection. An alternative asymmetric figure-of-eight single-layer suture anastomotic technique was introduced and its effects were evaluated in an<jats:italic>in vitro<\/jats:italic>porcine model. Twelve 15-cm grossly healthy small intestine segments from a porcine cadaver were harvested and randomly divided into asymmetric figure-of-eight single-layer suture (figure-of-eight suture) and single-layer interrupted suture technique (interrupted suture) groups (<jats:italic>n<\/jats:italic>\u2009=\u20096 in each group). The anastomosed bowel was infused with methylene blue solution to test anastomotic leakage. Anastomosis construction time, leakage, and suture material cost were recorded and analyzed statistically using Fisher's exact test and Student's<jats:italic>t<\/jats:italic>-test. One anastomotic leakage occurred (16.67%) in the figure-of-eight suture group, and two (33.33%) in the interrupted suture group (<jats:italic>p<\/jats:italic>\u2009&amp;gt;\u20090.9999). The anastomosis construction time was relatively short in the figure-of-eight suture group, but the difference did not reach a statistically significant level between the two groups. The mean number of suture knots and the cost of suture material in the figure-of-eight suture group were significantly decreased in comparison to the interrupted suture group (15.67\u2009\u00b1\u20093.30 vs. 22.17\u2009\u00b1\u20092.03, 167.11\u2009\u00b1\u200935.20 vs. 236.45\u2009\u00b1\u200921.70 CNY,<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.01, respectively). Our results suggested that the alternative asymmetric figure-of-eight suture technique was safe and economic for intestinal anastomosis. An<jats:italic>in vivo<\/jats:italic>experiment is required to elucidate the effects of this suture technique on the physiological anastomotic healing process.<\/jats:p>","DOI":"10.3389\/fsurg.2022.896542","type":"journal-article","created":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T05:50:25Z","timestamp":1664344225000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["An alternative asymmetric figure-of-eight single-layer suture technique for bowel anastomosis in an in vitro porcine model"],"prefix":"10.3389","volume":"9","author":[{"given":"Chen","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yewen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ai-rong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng-ai","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qizhong","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoxiu","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guojian","family":"Ding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingliang","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Geng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongshan","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1177\/145749691210100403","article-title":"The art of bowel anastomosis","volume":"101","author":"Chen","year":"2012","journal-title":"Scand J Surg"},{"key":"B2","doi-asserted-by":"publisher","first-page":"71","DOI":"10.5326\/JAAHA-MS-6459","article-title":"A comparison of ex vivo leak pressures for four enterotomy closures in a canine model","volume":"54","author":"Kieves","year":"2018","journal-title":"J Am Anim Hosp Assoc"},{"key":"B3","doi-asserted-by":"publisher","first-page":"106001","DOI":"10.1117\/1.JBO.20.10.106001","article-title":"Multispectral tissue characterization for intestinal anastomosis optimization","volume":"20","author":"Cha","year":"2015","journal-title":"J Biomed Opt"},{"key":"B4","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1007\/DCR.0b013e318208b90f","article-title":"A new antimesenteric functional end-to-end handsewn anastomosis: surgical prevention of anastomotic recurrence in Crohn's disease","volume":"54","author":"Kono","year":"2011","journal-title":"Dis Colon Rectum"},{"key":"B5","doi-asserted-by":"publisher","first-page":"94","DOI":"10.3760\/cma.j.issn.0253-3006.1997.02.011","article-title":"Experimental research of modified single-layer intestinal anastomosis and its clinical application in neonates with intestinal atresia","volume":"18","author":"Xu","year":"1997","journal-title":"Chin J Pediatr Surg"},{"key":"B6","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1308\/rcsann.2019.0106","article-title":"Benefits of barbed suture utilisation in gastrointestinal anastomosis: a systematic review and meta-analysis","volume":"102","author":"Wiggins","year":"2020","journal-title":"Ann R Coll Surg Engl"},{"key":"B7","doi-asserted-by":"publisher","first-page":"1757","DOI":"10.1016\/S0022-3468(98)90279-2","article-title":"Intestinal anastomosis in children: a comparative study between two different techniques","volume":"33","author":"Ordorica-Flores","year":"1998","journal-title":"J Pediatr Surg"},{"key":"B8","first-page":"175","article-title":"Digestive anastomosis in general surgery","volume":"19","author":"Natale","year":"1998","journal-title":"G Chir"},{"key":"B9","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1111\/j.2042-3306.2011.00494.x","article-title":"In vitro comparison of three suture techniques for anastomosis of the equine small intestine","volume":"40","author":"Auletta","year":"2011","journal-title":"Equine Vet J Suppl"},{"key":"B10","doi-asserted-by":"publisher","first-page":"PC01","DOI":"10.7860\/JCDR\/2017\/24817.9983","article-title":"Single layered versus double layered intestinal anastomosis: a randomized controlled trial","volume":"11","author":"Kar","year":"2017","journal-title":"J Clin Diagn Res"},{"key":"B11","doi-asserted-by":"publisher","first-page":"9","DOI":"10.4103\/ejs.ejs_78_17","article-title":"Is single-layer better than double-layer interrupted intestinal anastomosis? 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