{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T05:28:35Z","timestamp":1773293315672,"version":"3.50.1"},"reference-count":45,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T00:00:00Z","timestamp":1655337600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100019595","name":"scientific and technological innovation leading talents of \u201cTen Thousand Talents Program\u201d","doi-asserted-by":"publisher","award":["2018-WRJH-1"],"award-info":[{"award-number":["2018-WRJH-1"]}],"id":[{"id":"10.13039\/501100019595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011176","name":"discipline construction project of Peking Union Medical College","doi-asserted-by":"publisher","award":["xhxk201903"],"award-info":[{"award-number":["xhxk201903"]}],"id":[{"id":"10.13039\/501100011176","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"fellowship of China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2021M703654"],"award-info":[{"award-number":["2021M703654"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Surg."],"abstract":"<jats:p>Cell lineage tracing and single-cell sequencing have been widely applied in development biology and oncology to reveal the molecular mechanisms in multiple basic biological processes and the differentiation of stem cells, as well as quantify the differences between single cells. They provide new methods for in-depth understanding of the origin of tumors, the heterogeneity of tumor cells, and the drug resistance mechanism of tumors, thus inspiring new strategies for tumor treatment. In this review, we summarized the progress of cell lineage tracing technology and single-cell sequencing technology in the research of malignant melanoma and cutaneous squamous cell carcinoma, attempting to spark new ideas for further research on skin tumors.<\/jats:p>","DOI":"10.3389\/fsurg.2022.934828","type":"journal-article","created":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T07:02:30Z","timestamp":1655362950000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Research Progress of Cell Lineage Tracing and Single-Cell Sequencing Technology in Malignant Skin Tumors"],"prefix":"10.3389","volume":"9","author":[{"given":"Ang","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baoyi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingkai","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,6,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.cell.2012.01.002","article-title":"Lineage tracing","volume":"148","author":"Kretzschmar","year":"2012","journal-title":"Cell"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1820","DOI":"10.1038\/s41596-019-0163-5","article-title":"Using Cre-recombinase-driven Polylox barcoding for in vivo fate mapping in mice","volume":"14","author":"Pei","year":"2019","journal-title":"Nat Protoc"},{"key":"B3","doi-asserted-by":"publisher","first-page":"104198","DOI":"10.1016\/j.meegid.2020.104198","article-title":"Progress and applications of single-cell sequencing techniques","volume":"80","author":"Yasen","year":"2020","journal-title":"Infect Genet Evol"},{"key":"B4","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1038\/nmeth.1315","article-title":"mRNA-Seq whole-transcriptome analysis of a single cell","volume":"6","author":"Tang","year":"2009","journal-title":"Nat Methods"},{"key":"B5","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1016\/j.molcel.2018.10.020","article-title":"Comparative analysis of droplet-based ultra-high-throughput single-cell RNA-seq systems","volume":"73","author":"Zhang","year":"2019","journal-title":"Mol Cell"},{"key":"B6","doi-asserted-by":"publisher","first-page":"2886","DOI":"10.1073\/pnas.1919800117","article-title":"RNA sequencing by direct tagmentation of RNA\/DNA hybrids","volume":"117","author":"Di","year":"2020","journal-title":"Proc Natl Acad Sci U S A"},{"key":"B7","doi-asserted-by":"publisher","first-page":"602","DOI":"10.2174\/1389202921999200625220812","article-title":"The comparison of two single-cell sequencing platforms: BD rhapsody and 10x genomics chromium","volume":"21","author":"Gao","year":"2020","journal-title":"Curr Genomics"},{"key":"B8","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1038\/nature23653","article-title":"Polylox barcoding reveals haematopoietic stem cell fates realized in vivo","volume":"548","author":"Pei","year":"2017","journal-title":"Nature"},{"key":"B9","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1016\/j.stem.2020.07.018","article-title":"Resolving fates and single-cell transcriptomes of hematopoietic stem cell clones by PolyloxExpress barcoding","volume":"27","author":"Pei","year":"2020","journal-title":"Cell Stem Cell"},{"key":"B10","doi-asserted-by":"publisher","first-page":"209","DOI":"10.3322\/caac.21660","article-title":"Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J Clin"},{"key":"B11","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1038\/nature22071","article-title":"Whole-genome landscapes of major melanoma subtypes","volume":"545","author":"Hayward","year":"2017","journal-title":"Nature"},{"key":"B12","doi-asserted-by":"publisher","first-page":"706-e1","DOI":"10.1016\/j.ccr.2013.05.001","article-title":"SnapShot: melanoma","volume":"23","author":"Vultur","year":"2013","journal-title":"Cancer Cell"},{"key":"B13","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1684\/ejd.2016.2787","article-title":"Genetic epidemiology of melanoma","volume":"26","author":"Ribero","year":"2016","journal-title":"Eur J Dermatol"},{"key":"B14","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1034\/j.1600-0749.2002.02027.x","article-title":"Redefining the skin\u2019s pigmentary system with a novel tyrosinase assay","volume":"15","author":"Han","year":"2002","journal-title":"Pigment Cell Res"},{"key":"B15","doi-asserted-by":"publisher","first-page":"544","DOI":"10.1038\/ng.356","article-title":"Braf(V600E) cooperates with Pten loss to induce metastatic melanoma","volume":"41","author":"Dankort","year":"2009","journal-title":"Nat Genet"},{"key":"B16","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1016\/j.stem.2017.10.009","article-title":"Location, location, location: spatio-temporal cues that define the cell of origin in melanoma","volume":"21","author":"Soengas","year":"2017","journal-title":"Cell Stem Cell"},{"key":"B17","doi-asserted-by":"publisher","first-page":"679","DOI":"10.1016\/j.stem.2017.08.003","article-title":"Mouse cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation of mature pigmented melanocytes","volume":"21","author":"Kohler","year":"2017","journal-title":"Cell Stem Cell"},{"key":"B18","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1016\/j.stem.2017.09.001","article-title":"Melanocyte stem cell activation and translocation initiate cutaneous melanoma in response to UV exposure","volume":"21","author":"Moon","year":"2017","journal-title":"Cell Stem Cell"},{"key":"B19","doi-asserted-by":"publisher","first-page":"865","DOI":"10.1038\/s41587-021-00837-3","article-title":"Variability within rare cell states enables multiple paths toward drug resistance","volume":"39","author":"Emert","year":"2021","journal-title":"Nat Biotechnol"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10030506","article-title":"Melanoma single-cell biology in experimental and clinical settings","volume":"10","author":"Binder","year":"2021","journal-title":"J Clin Med"},{"key":"B21","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1126\/science.aad0501","article-title":"Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq","volume":"352","author":"Tirosh","year":"2016","journal-title":"Science"},{"key":"B22","doi-asserted-by":"publisher","first-page":"998","DOI":"10.1016\/j.cell.2018.10.038","article-title":"Defining T cell states associated with response to checkpoint immunotherapy in melanoma","volume":"175","author":"Sade-Feldman","year":"2018","journal-title":"Cell"},{"key":"B23","doi-asserted-by":"publisher","first-page":"984","DOI":"10.1016\/j.cell.2018.09.006","article-title":"A cancer cell program promotes T cell exclusion and resistance to checkpoint blockade","volume":"175","author":"Jerby-Arnon","year":"2018","journal-title":"Cell"},{"key":"B24","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1016\/j.cell.2018.11.043","article-title":"Dysfunctional CD8 T cells form a proliferative, dynamically regulated compartment within human melanoma","volume":"176","author":"Li","year":"2019","journal-title":"Cell"},{"key":"B25","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1038\/s42003-020-0888-2","article-title":"Single-cell analysis of a mutant library generated using CRISPR-guided deaminase in human melanoma cells","volume":"3","author":"Jun","year":"2020","journal-title":"Commun Biol"},{"key":"B26","doi-asserted-by":"publisher","first-page":"1669","DOI":"10.1038\/s41467-021-21928-4","article-title":"Circulating mucosal-associated invariant T cells identify patients responding to anti-PD-1 therapy","volume":"12","author":"De Biasi","year":"2021","journal-title":"Nat Commun"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20200920","article-title":"Single-cell analyses identify circulating anti-tumor CD8 T cells and markers for their enrichment","volume":"218","author":"Pauken","year":"2021","journal-title":"J Exp Med"},{"key":"B28","doi-asserted-by":"publisher","first-page":"1938","DOI":"10.1158\/2159-8290.CD-20-0555","article-title":"Splicing patterns in SF3B1-mutated Uveal melanoma generate shared immunogenic tumor-specific neoepitopes","volume":"11","author":"Bigot","year":"2021","journal-title":"Cancer Discov"},{"key":"B29","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1016\/j.cell.2018.06.025","article-title":"Toward minimal residual disease-directed therapy in melanoma","volume":"174","author":"Rambow","year":"2018","journal-title":"Cell"},{"key":"B30","doi-asserted-by":"publisher","first-page":"2131","DOI":"10.1158\/1078-0432.CCR-21-3145","article-title":"Single cell characterization of the cellular landscape of acral melanoma identifies novel targets for immunotherapy","volume":"28","author":"Li","year":"2022","journal-title":"Clin Cancer Res"},{"key":"B31","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1016\/j.jaad.2017.08.059","article-title":"Cutaneous squamous cell carcinoma: Incidence, risk factors, diagnosis, and staging","volume":"78","author":"Que","year":"2018","journal-title":"J Am Acad Dermatol"},{"key":"B32","doi-asserted-by":"publisher","first-page":"6582","DOI":"10.1158\/1078-0432.CCR-14-1768","article-title":"Mutational landscape of aggressive cutaneous squamous cell carcinoma","volume":"20","author":"Pickering","year":"2014","journal-title":"Clin Cancer Res"},{"key":"B33","doi-asserted-by":"publisher","first-page":"7431","DOI":"10.1073\/pnas.1012720108","article-title":"Identifying the cellular origin of squamous skin tumors","volume":"108","author":"Lapouge","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"key":"B34","doi-asserted-by":"publisher","first-page":"7425","DOI":"10.1073\/pnas.1012670108","article-title":"Defining the origins of Ras\/p53-mediated squamous cell carcinoma","volume":"108","author":"White","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"key":"B35","doi-asserted-by":"publisher","first-page":"5317","DOI":"10.1158\/0008-5472.CAN-11-1059","article-title":"Cancer stem cells in squamous cell carcinoma switch between two distinct phenotypes that are preferentially migratory or proliferative","volume":"71","author":"Biddle","year":"2011","journal-title":"Cancer Res"},{"key":"B36","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.stem.2016.10.018","article-title":"Cell-type-specific chromatin states differentially prime squamous cell carcinoma tumor-initiating cells for epithelial to mesenchymal transition","volume":"20","author":"Latil","year":"2017","journal-title":"Cell Stem Cell"},{"key":"B37","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1038\/s41568-018-0024-5","article-title":"Deciphering the cells of origin of squamous cell carcinomas","volume":"18","author":"Sanchez-Danes","year":"2018","journal-title":"Nat Rev Cancer"},{"key":"B38","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1016\/j.stem.2019.01.003","article-title":"De Novo PITX1 expression controls bi-stable transcriptional circuits to govern self-renewal and differentiation in squamous cell carcinoma","volume":"24","author":"Sastre-Perona","year":"2019","journal-title":"Cell Stem Cell"},{"key":"B39","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1038\/s41419-021-04477-y","article-title":"Single-cell transcriptomic analysis reveals the critical molecular pattern of UV-induced cutaneous squamous cell carcinoma","volume":"13","author":"Yan","year":"2021","journal-title":"Cell Death Dis"},{"key":"B40","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/j.cell.2020.05.039","article-title":"Multimodal analysis of composition and spatial architecture in human squamous cell carcinoma","volume":"182","author":"Ji","year":"2020","journal-title":"Cell"},{"key":"B41","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1038\/s41698-020-0119-9","article-title":"Decreased cytotoxic T cells and TCR clonality in organ transplant recipients with squamous cell carcinoma","volume":"4","author":"Frazzette","year":"2020","journal-title":"NPJ Precis Oncol"},{"key":"B42","doi-asserted-by":"publisher","first-page":"1172","DOI":"10.1016\/j.cell.2019.03.025","article-title":"Adaptive immune resistance emerges from tumor-initiating stem cells","volume":"177","author":"Miao","year":"2019","journal-title":"Cell"},{"key":"B43","doi-asserted-by":"publisher","first-page":"1251","DOI":"10.1038\/s41591-019-0522-3","article-title":"Clonal replacement of tumor-specific T cells following PD-1 blockade","volume":"25","author":"Yost","year":"2019","journal-title":"Nat Med"},{"key":"B44","doi-asserted-by":"publisher","first-page":"1597","DOI":"10.1016\/j.stem.2021.04.011","article-title":"CD276 expression enables squamous cell carcinoma stem cells to evade immune surveillance","volume":"28","author":"Wang","year":"2021","journal-title":"Cell Stem Cell"},{"key":"B45","doi-asserted-by":"publisher","first-page":"1410","DOI":"10.1016\/j.cell.2020.04.048","article-title":"An engineered CRISPR-Cas9 mouse line for simultaneous readout of lineage histories and gene expression profiles in single cells","volume":"181","author":"Bowling","year":"2020","journal-title":"Cell"}],"container-title":["Frontiers in Surgery"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fsurg.2022.934828\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T07:02:33Z","timestamp":1655362953000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fsurg.2022.934828\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,16]]},"references-count":45,"alternative-id":["10.3389\/fsurg.2022.934828"],"URL":"https:\/\/doi.org\/10.3389\/fsurg.2022.934828","relation":{},"ISSN":["2296-875X"],"issn-type":[{"value":"2296-875X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,16]]},"article-number":"934828"}}