{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T15:35:16Z","timestamp":1782228916794,"version":"3.54.5"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T00:00:00Z","timestamp":1663632000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Surg."],"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>To detect the composition of the gut microbiota in biliary atresia after Kasai surgery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Infants within six months after the Kasai operation who were diagnosed by cholangiography at Shanghai Children\u2019s Hospital were enrolled in the study. Fecal samples were collected from diapers, placed into sterile tubes in the inpatient department or outpatient department and frozen at \u221280\u00b0C within half an hour. The gut microbiota was detected by 16S rRNA sequences. Then, the patients that were followed up to one year after the Kasai operation who suffered from cholangitis at least one time were grouped into the BAcho group, and the others were grouped into the BAnoncho group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Nine of 18 BA patients were grouped into the BAcho group, and the others were grouped into the BAnoncho group. In the BAcho group, AST, ALT and GGT were significantly increased compared to the BAnoncho group. The number of total OTUs (operational taxonomic units) in feces was more elevated in the BAnoncho group than in the BAcho group. In the BAnoncho group, the Chao index at the OTU level was significantly increased compared to that in the BAcho group (66.37\u2009\u00b1\u200921.5 vs. 45.64\u2009\u00b1\u200911.25, <jats:italic>p<\/jats:italic>\u2009=\u20090.02\u2009&amp;lt;\u20090.05). <jats:italic>Bifidobacterium<\/jats:italic> was the most abundant genus in the BAnoncho group, accounting for 22.14%, and <jats:italic>Klebsiella<\/jats:italic> accounted for 22.74% in the BAcho group. Compared with the BAnoncho group, <jats:italic>Bacteroides<\/jats:italic> was significantly decreased in the BAcho group (<jats:italic>p<\/jats:italic>\u2009=\u20090.037).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The composition of the gut microbiota was different between BA with cholangitis and BA without cholangitis.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fsurg.2022.990101","type":"journal-article","created":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T05:24:13Z","timestamp":1663651453000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["The composition of the gut microbiota is altered in biliary atresia with cholangitis"],"prefix":"10.3389","volume":"9","author":[{"given":"Lulu","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yibo","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenhua","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhibao","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijue","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingfeng","family":"Sheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangbin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/S0140-6736(99)03492-3","article-title":"The frequency and outcome of biliary atresia in the UK and Ireland","volume":"355","author":"McKiernan","year":"2000","journal-title":"Lancet"},{"key":"B2","doi-asserted-by":"publisher","first-page":"FSO466","DOI":"10.2144\/fsoa-2019-0153","article-title":"Biliary atresia: pathology, etiology and pathogenesis","volume":"6","author":"Vij","year":"2020","journal-title":"Future Sci OA"},{"key":"B3","doi-asserted-by":"publisher","first-page":"1086","DOI":"10.1016\/j.cgh.2011.07.024","article-title":"Twenty-year transplant-free survival rate among patients with biliary atresia","volume":"9","author":"de Vries","year":"2011","journal-title":"Clin Gastroenterol Hepatol"},{"key":"B4","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1016\/j.amsu.2021.01.052","article-title":"Short- and long-term outcomes after Kasai operation for type III biliary atresia: twenty years of experience in a single tertiary Egyptian center-A retrospective cohort study","volume":"62","author":"Gad","year":"2021","journal-title":"Ann Med Surg"},{"key":"B5","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1586\/egh.12.37","article-title":"Early detection of biliary atresia: past, present \/ future","volume":"6","author":"Muraji","year":"2012","journal-title":"Expert Rev Gastroenterol Hepatol"},{"key":"B6","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1007\/s00104-019-01058-w","article-title":"Gallenwegschirurgie im Kindesalte. 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