{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:56:54Z","timestamp":1790751414554,"version":"4.1.0"},"reference-count":21,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Surg."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Intestinal ischemia-reperfusion contributes to barrier failure and secondary organ injury after hemorrhagic shock. It is not known whether restrictive hypertonic saline resuscitation alters the resulting intestinal injury or the accompanying non-coding RNA response.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>One hundred male Sprague\u2013Dawley rats were allocated to normal control, hemorrhagic shock, conventional 0.9% saline resuscitation (CFR), restrictive 7.5% hypertonic saline resuscitation (RFR), or superior mesenteric artery ischemia-reperfusion control groups. Hemorrhagic shock was induced by controlled blood withdrawal to maintain mean arterial pressure (MAP) at 35\u221240\u2005mmHg for 60\u2005min. CFR targeted an MAP of approximately 80 mmHg, whereas RFR targeted an MAP of approximately 60\u2005mmHg. Three-hour survival was recorded. Coagulation, serum IL-6 and TNF-\u03b1, qRT-PCR, Western blotting, H&amp;amp;E staining, and TUNEL were assessed in assay-specific subsets. Public GEO datasets were analyzed for exploratory transcriptomic context.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Three-hour survival was 75% in RFR and 60% in CFR (two-sided Fisher exact test,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.501). In the coagulation cohort, prothrombin time (PT) and thrombin time (TT) were shorter in RFR than in CFR after Dunn\u2013Holm adjustment; no differences were observed for activated partial thromboplastin time (APTT), international normalized ratio (INR), and prothrombin activity (PA). RFR had lower serum IL-6 and TNF-\u03b1, better-preserved ileal morphology, and lower TUNEL positivity in representative fields than CFR. MALAT1 was lower, while miR-145-5p and Bnip3\/BNIP3 were higher in RFR. Exploratory analyses of public datasets confirmed that Malat1 and Bnip3 are expressed in the intestinal cell populations relevant to ischemia-reperfusion injury.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Under the present experimental conditions, restrictive hypertonic saline resuscitation was associated with a less severe acute intestinal injury phenotype than conventional isotonic resuscitation. The coordinated changes in MALAT1, miR-145-5p, and BNIP3 do not establish a linear regulatory axis and require functional validation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fsurg.2026.1920366","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:06:23Z","timestamp":1790748383000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Restrictive hypertonic saline resuscitation is associated with less intestinal injury after hemorrhagic shock in rats and with coordinated changes in MALAT1, miR-145-5p, and BNIP3 expression"],"prefix":"10.3389","volume":"13","author":[{"given":"Hongfei","family":"Ma","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Xinjiang Medical University","place":["Urumqi, Xinjiang Uygur Autonomous Region, 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