{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T20:04:12Z","timestamp":1791921852792,"version":"4.3.4"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Toxicol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are persistent environmental pollutants, but their role in bladder epithelial injury remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>SV-HUC-1 cells were exposed to PFOA and PFOS. Cell viability, lipid peroxidation, and ROS levels were assessed using CCK-8 assay, flow cytometry, and transmission electron microscopy. Protein expression and ubiquitination were analyzed by Western blotting and molecular docking.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>PFOA and PFOS induced ferroptosis in bladder epithelial cells, characterized by increased lipid peroxidation and ROS levels. Mechanistically, PFOA\/PFOS inhibited ACSL4 ubiquitination at K593 and K690, leading to its stabilization and promoting ferroptosis. Knockdown of ACSL4 significantly reversed these effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings reveal a novel mechanism by which PFAS induce ferroptosis via regulation of ACSL4 ubiquitination, providing new insights into PFAS-induced bladder toxicity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/ftox.2026.1774625","type":"journal-article","created":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T22:10:20Z","timestamp":1778451020000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["PFOA\/PFOS induce ferroptosis in bladder epithelial cells through inhibition of ACSL4 ubiquitination"],"prefix":"10.3389","volume":"8","author":[{"given":"Hao","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Urology, Huangshi Central Hospital, Affiliated Hospital of 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China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengwei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University","place":["Huangshi, Hubei, China"]},{"name":"Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention","place":["Huangshi, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghua","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Urology, Huangshi Central Hospital Graduate Joint Training Base, School of Medicine, Wuhan University of Science and Technology","place":["Huangshi, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology, Huangshi Central Hospital Graduate Joint Training Base, School of Medicine, Wuhan University of Science and 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