{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T10:56:54Z","timestamp":1791975414338,"version":"4.4.0"},"reference-count":199,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T00:00:00Z","timestamp":1566864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"DOI":"10.3389\/fvets.2019.00268","type":"journal-article","created":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T00:38:36Z","timestamp":1566866316000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":205,"title":["Advances in Cryopreservation of Bull Sperm"],"prefix":"10.3389","volume":"6","author":[{"given":"Muhammet Rasit","family":"Ugur","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal","family":"Saber Abdelrahman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holly C.","family":"Evans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia A.","family":"Gilmore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa","family":"Hitit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raden Iis","family":"Arifiantini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bambang","family":"Purwantara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullah","family":"Kaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erdogan","family":"Memili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2019,8,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1016\/S0093-691X(01)00674-4","article-title":"Current status of sperm cryopreservation: why isn't it better?","volume":"57","author":"Medeiros","year":"2002","journal-title":"Theriogenology."},{"key":"B2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/j.1939-4640.2000.tb03268.x","article-title":"Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon","volume":"21","author":"Bailey","year":"2000","journal-title":"J Androl."},{"key":"B3","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/0011-2240(64)90002-1","article-title":"Low temperature research on spermatozoa and eggs","volume":"1","author":"Sherman","year":"1964","journal-title":"Cryobiology."},{"key":"B4","first-page":"137","article-title":"Artificial insemination history: hurdles and milestones","volume":"7","author":"Ombelet","year":"2015","journal-title":"Facts Vis Obgyn."},{"key":"B5","doi-asserted-by":"publisher","first-page":"399","DOI":"10.3168\/jds.S.0022-0302(40)95541-2","article-title":"A yolk-buffer pabulum for the preservation of bull semen","volume":"23","author":"Phillips","year":"1940","journal-title":"J Dairy Sci."},{"key":"B6","doi-asserted-by":"publisher","first-page":"905","DOI":"10.3168\/jds.S0022-0302(41)95476-0","article-title":"Preservation of bovine spermatozoa in yolk-citrate diluent and field results from its use","volume":"24","author":"Salisbury","year":"1941","journal-title":"J Dairy Sci."},{"key":"B7","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1038\/164666a0","article-title":"Revival of spermatozoa after vitrification and dehydration at low temperatures","volume":"164","author":"Polge","year":"1949","journal-title":"Nature."},{"key":"B8","doi-asserted-by":"crossref","unstructured":"WaltersEM BensonJD WoodsEJ CritserJK The History of Sperm Cryopreservation Importance of Sperm Cryopreservation.2009","DOI":"10.1017\/CBO9781139193771.002"},{"key":"B9","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1016\/S1472-6483(10)60073-9","article-title":"Influence of freeze-thawing on hyaluronic acid binding of human spermatozoa","volume":"19","author":"Nijs","year":"2009","journal-title":"Reprod Biomed Online."},{"key":"B10","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1002\/j.1939-4640.1990.tb01583.x","article-title":"Cryopreservation of mammalian sperm: what we ask them to survive","volume":"11","author":"Hammerstedt","year":"1990","journal-title":"J Androl."},{"key":"B11","doi-asserted-by":"publisher","first-page":"90","DOI":"10.4161\/org.5.3.10021","article-title":"Cryopreservation: an emerging paradigm change","volume":"5","author":"Baust","year":"2009","journal-title":"Organogenesis."},{"key":"B12","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1111\/j.1439-0531.1995.tb00016.x","article-title":"Cooling of spermatozoa and fertilizing capacity","volume":"31","author":"Watson","year":"1995","journal-title":"Reprod Domest Anim."},{"key":"B13","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/0011-2240(90)90009-S","article-title":"Cold-induced ultrastructural changes in bull and boar sperm plasma membranes","volume":"27","author":"De Leeuw","year":"1990","journal-title":"Cryobiology."},{"key":"B14","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1016\/j.rbmo.2010.05.011","article-title":"Implication of apoptosis in sperm cryoinjury","volume":"21","author":"Said","year":"2010","journal-title":"Reprod Biomed Online."},{"key":"B15","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1071\/RD9950659","article-title":"Free radicals, lipid peroxidation and sperm function","volume":"7","author":"Aitken","year":"1995","journal-title":"Reprod Fertil Dev."},{"key":"B16","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1007\/978-1-60327-378-7_24","article-title":"Markers of Oxidative Stress and Sperm Chromatin Integrity","volume-title":"Molecular Endocrinology. Methods in Molecular Biology (Methods and Protocols), Vol 590","author":"Agarwal","year":"2009"},{"key":"B17","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1007\/s00441-005-1097-5","article-title":"DNA damage to spermatozoa has impacts on fertilization and pregnancy","volume":"322","author":"Lewis","year":"2005","journal-title":"Cell Tissue Res."},{"key":"B18","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/j.theriogenology.2007.05.001","article-title":"Is there a relationship between the chromatin status and DNA fragmentation of boar spermatozoa following freezing-thawing?","volume":"68","author":"Fraser","year":"2007","journal-title":"Theriogenology."},{"key":"B19","doi-asserted-by":"publisher","first-page":"1613","DOI":"10.1016\/j.theriogenology.2011.01.001","article-title":"DNA fragmentation in chicken spermatozoa during cryopreservation","volume":"75","author":"Gliozzi","year":"2011","journal-title":"Theriogenology."},{"key":"B20","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1095\/biolreprod.109.080507","article-title":"Osmotic stress induces oxidative cell damage to rhesus macaque spermatozoa1","volume":"82","author":"McCarthy","year":"2009","journal-title":"Biol Reprod."},{"key":"B21","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1111\/andr.12279","article-title":"Identification of protein changes in human spermatozoa throughout the cryopreservation process","volume":"5","author":"Bogle","year":"2017","journal-title":"Andrology"},{"key":"B22","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1093\/humupd\/dmu063","article-title":"The effect of cryopreservation on the genome of gametes and embryos: principles of cryobiology and critical appraisal of the evidence","volume":"21","author":"Kopeika","year":"2015","journal-title":"Hum Reprod Update"},{"key":"B23","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1530\/REP-11-0436","article-title":"Osmotic stress and cryoinjury of koala sperm: an integrative study of the plasma membrane, chromatin stability and mitochondrial function","volume":"143","author":"Johnston","year":"2012","journal-title":"Reproduction"},{"key":"B24","doi-asserted-by":"crossref","first-page":"389","DOI":"10.56093\/ijans.v84i4.39838","article-title":"Characteristics of frozen thawed semen in predicting the fertility of buffalo bulls","volume":"84","author":"Kumar","year":"2014","journal-title":"Indian J Anim Sci."},{"key":"B25","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1038\/429154a","article-title":"Reproductive biology: delivering spermatozoan RNA to the oocyte","volume":"429","author":"Ostermeier","year":"2004","journal-title":"Nature."},{"key":"B26","doi-asserted-by":"publisher","first-page":"1034","DOI":"10.1101\/gr.201541.115","article-title":"Sperm is epigenetically programmed to regulate gene transcription in embryos","volume":"26","author":"Teperek","year":"2016","journal-title":"Genome Res."},{"key":"B27","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.cryobiol.2014.06.003","article-title":"A preliminary study on epigenetic changes during boar spermatozoa cryopreservation","volume":"69","author":"Zeng","year":"2014","journal-title":"Cryobiology."},{"key":"B28","doi-asserted-by":"publisher","first-page":"67394","DOI":"10.18632\/oncotarget.18672","article-title":"Sperm-carried RNAs play critical roles in mouse embryonic development","volume":"8","author":"Guo","year":"2017","journal-title":"Oncotarget."},{"key":"B29","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/j.gene.2015.12.020","article-title":"Small non-coding RNAs and their associated proteins in spermatogenesis","volume":"578","author":"Luo","year":"2016","journal-title":"Gene."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1095\/biolreprod.112.103788","article-title":"Cryopreserved bovine spermatozoal transcript profile as revealed by high-throughput ribonucleic acid sequencing1","volume":"88","author":"Card","year":"2013","journal-title":"Biol Reprod."},{"key":"B31","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.cryobiol.2013.05.007","article-title":"Effect of cryopreservation on human sperm messenger RNAs crucial for fertilization and early embryo development","volume":"67","author":"Valcarce","year":"2013","journal-title":"Cryobiology."},{"key":"B32","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.anireprosci.2016.11.003","article-title":"Implementing an open-access CASA software for the assessment of stallion sperm motility: relationship with other sperm quality parameters","volume":"176","author":"Giaretta","year":"2017","journal-title":"Anim Reprod Sci."},{"key":"B33","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/j.rbmo.2011.04.006","article-title":"Effects of cryopreservation and density-gradient washing on phospholipase C zeta concentrations in human spermatozoa","volume":"23","author":"Kashir","year":"2011","journal-title":"Reprod Biomed Online"},{"key":"B34","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1017\/S096719941300004X","article-title":"HSP90 expression correlation with the freezing resistance of bull sperm","volume":"22","author":"Wang","year":"2014","journal-title":"Zygote."},{"key":"B35","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1016\/j.theriogenology.2014.10.015","article-title":"Comparative transcript profiling of gene expression of fresh and frozen-thawed bull sperm","volume":"83","author":"Chen","year":"2015","journal-title":"Theriogenology."},{"key":"B36","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1186\/s12864-016-3394-7","article-title":"Small RNA sequencing of cryopreserved semen from single bull revealed altered miRNAs and piRNAs expression between High- and Low-motile sperm populations","volume":"18","author":"Capra","year":"2017","journal-title":"BMC Genomics."},{"key":"B37","doi-asserted-by":"publisher","first-page":"802","DOI":"10.3390\/ijms20040802","article-title":"Exploration of miRNA and mRNA profiles in fresh and frozen-thawed boar sperm by transcriptome and small RNA sequencing","volume":"20","author":"Dai","year":"2019","journal-title":"Int J Mol Sci."},{"key":"B38","doi-asserted-by":"publisher","first-page":"3066","DOI":"10.3390\/ijms19103066","article-title":"Transcriptome Sequencing Reveals the Differentially Expressed lncRNAs and mRNAs Involved in Cryoinjuries in frozen-thawed giant panda (Ailuropoda melanoleuca) Sperm","volume":"19","author":"Ran","year":"2018","journal-title":"Int J Mol Sci."},{"key":"B39","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.cryobiol.2017.04.013","article-title":"Cryopreservation of boar sperm induces differential microRNAs expression","volume":"76","author":"Zhang","year":"2017","journal-title":"Cryobiology."},{"key":"B40","doi-asserted-by":"publisher","first-page":"e0213420","DOI":"10.1371\/journal.pone.0213420","article-title":"Transcriptome analysis reveals that fertilization with cryopreserved sperm downregulates genes relevant for early embryo development in the horse","volume":"14","author":"Ortiz-Rodriguez","year":"2019","journal-title":"PLoS ONE"},{"key":"B41","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1111\/andr.12001","article-title":"Sperm global DNA methylation level: Association with semen parameters and genome integrity","volume":"3","author":"Montjean","year":"2015","journal-title":"Andrology."},{"key":"B42","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1186\/s12864-017-3673-y","article-title":"Male fertility status is associated with DNA methylation signatures in sperm and transcriptomic profiles of bovine preimplantation embryos","volume":"18","author":"Kropp","year":"2017","journal-title":"BMC Genom."},{"key":"B43","doi-asserted-by":"publisher","first-page":"1347","DOI":"10.1016\/j.theriogenology.2016.04.077","article-title":"Cytosine methylation of sperm DNA in horse semen after cryopreservation","volume":"86","author":"Aurich","year":"2016","journal-title":"Theriogenology."},{"key":"B44","doi-asserted-by":"publisher","first-page":"e67614","DOI":"10.1371\/journal.pone.0067614","article-title":"Cryopreservation causes genetic and epigenetic changes in zebrafish genital ridges","volume":"8","author":"Riesco","year":"2013","journal-title":"PLoS ONE."},{"key":"B45","doi-asserted-by":"publisher","first-page":"R55","DOI":"10.1530\/REP-15-0562","article-title":"Epigenetic regulation of the histone-to-protamine transition during spermiogenesis","volume":"151","author":"Bao","year":"2016","journal-title":"Reproduction."},{"key":"B46","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.anireprosci.2016.01.017","article-title":"The sperm chromatin structure assay (SCSA\u00ae) and other sperm DNA fragmentation tests for evaluation of sperm nuclear DNA integrity as related to fertility","volume":"169","author":"Evenson","year":"2016","journal-title":"Anim Reprod Sci."},{"key":"B47","doi-asserted-by":"publisher","first-page":"958","DOI":"10.1016\/j.theriogenology.2005.09.010","article-title":"Detection of damage in mammalian sperm cells","volume":"65","author":"Silva","year":"2006","journal-title":"Theriogenology."},{"key":"B48","doi-asserted-by":"publisher","first-page":"S49","DOI":"10.1016\/j.ijvsm.2017.11.001","article-title":"Evaluation of bull spermatozoa during and after cryopreservation: structural and ultrastructural insights","volume":"6","author":"Khalil","year":"2018","journal-title":"Int J Vet Sci Med."},{"key":"B49","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1095\/biolreprod66.2.354","article-title":"Sperm apoptosis in fresh and cryopreserved bull semen detected by flow cytometry and its relationship with fertility 1","volume":"66","author":"Anzar","year":"2002","journal-title":"Biol Reprod."},{"key":"B50","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1186\/s40104-016-0076-x","article-title":"Sperm cryodamage occurs after rapid freezing phase: flow cytometry approach and antioxidant enzymes activity at different stages of cryopreservation","volume":"5","author":"Castro","year":"2016","journal-title":"J Anim Sci Biotechnol."},{"key":"B51","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1046\/j.1365-2605.2001.00270.x","article-title":"Comparison between computerized slow-stage and static liquid nitrogen vapour freezing methods with respect to the deleterious effect on chromatin and morphology of spermatozoa from fertile and subfertile men","volume":"24","author":"Hammadeh","year":"2001","journal-title":"Int J Androl."},{"key":"B52","doi-asserted-by":"publisher","first-page":"191","DOI":"10.21451\/1984-3143-AR884","article-title":"Functional aspects of seminal plasma and sperm proteins and their potential as molecular markers of fertility","volume":"13","author":"Moura","year":"2016","journal-title":"Anim Reprod."},{"key":"B53","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.cryobiol.2014.12.010","article-title":"Association of heat shock protein 90 with motility of post-thawed sperm in bulls","volume":"70","author":"Zhang","year":"2015","journal-title":"Cryobiology."},{"key":"B54","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1016\/j.theriogenology.2015.05.004","article-title":"Heat shock protein A8 stabilizes the bull sperm plasma membrane during cryopreservation: effects of breed, protein concentration, and mode of use","volume":"84","author":"Holt","year":"2015","journal-title":"Theriogenology."},{"key":"B55","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.theriogenology.2017.01.011","article-title":"Cryopreservation of bull semen is associated with carbonylation of sperm proteins","volume":"92","author":"Mostek","year":"2017","journal-title":"Theriogenology."},{"key":"B56","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1016\/S0093-691X(03)00230-9","article-title":"Two-dimensional polyacrylamide gel electrophoresis of bovine seminal plasma proteins and their relation with semen freezability","volume":"61","author":"Jobim","year":"2004","journal-title":"Theriogenology."},{"key":"B57","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/0014-5793(94)00362-9","article-title":"Bovine seminal plasma ASFP: Localization of disulfide bridges and detection of three different isoelectric forms","volume":"344","author":"Einspanier","year":"1994","journal-title":"FEBS Lett."},{"key":"B58","doi-asserted-by":"crossref","first-page":"565","DOI":"10.5772\/33565","article-title":"Methods of assessment of cryopreserved semen","volume-title":"Current Frontiers in Cryobiology","author":"Partyka","year":"2012"},{"key":"B59","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1111\/j.1439-0531.2006.00623.x","article-title":"Studies on the effect of supplementing boar semen cryopreservation media with different avian egg yolk types on in vitro post-thaw sperm quality","volume":"41","author":"Bathgate","year":"2006","journal-title":"Reprod Domest Anim."},{"key":"B60","doi-asserted-by":"publisher","first-page":"1695","DOI":"10.1016\/S0093-691X(02)00682-9","article-title":"Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen\u2013thawed bull semen","volume":"57","author":"Moussa","year":"2002","journal-title":"Theriogenology."},{"key":"B61","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1016\/S0093-691X(03)00259-0","article-title":"Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with Optidyl\u00ae, a commercial egg yolk extender","volume":"61","author":"Amirat","year":"2004","journal-title":"Theriogenology."},{"key":"B62","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.anireprosci.2017.01.012","article-title":"The cryoprotective effect of iodixanol in buffalo semen cryopreservation","volume":"179","author":"Swami","year":"2017","journal-title":"Anim Reprod Sci."},{"key":"B63","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1016\/j.anireprosci.2018.02.010","article-title":"Comparison of plant- and egg yolk-based semen diluents on in vitro sperm kinematics and in vivo fertility of frozen-thawed bull semen","volume":"191","author":"Murphy","year":"2018","journal-title":"Anim Reprod Sci."},{"key":"B64","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.aaspro.2016.12.021","article-title":"Effects of extenders on fresh and freezing semen of boer goat","volume":"11","author":"Yodmingkwan","year":"2016","journal-title":"Agric Agric Sci Proc."},{"key":"B65","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.smallrumres.2012.07.022","article-title":"Soybean lecithin-based extender as an alternative for goat sperm cryopreservation","volume":"109","author":"Vidal","year":"2013","journal-title":"Small Rumin Res."},{"key":"B66","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1016\/S0093-691X(02)01369-9","article-title":"in vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen","volume":"60","author":"Aires","year":"2003","journal-title":"Theriogenology."},{"key":"B67","doi-asserted-by":"publisher","first-page":"1064","DOI":"10.1016\/j.theriogenology.2014.12.012","article-title":"Soybean lecithin\u2013based extender preserves spermatozoa membrane integrity and fertilizing potential during goat semen cryopreservation","volume":"83","author":"Chelucci","year":"2015","journal-title":"Theriogenology."},{"key":"B68","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1111\/j.1439-0531.2006.00784.x","article-title":"Post-thaw survival and longevity of bull spermatozoa frozen with an egg yolk-based or two egg yolk-free extenders after an equilibration period of 18\u00b0h","volume":"42","author":"Mui\u00f1o","year":"2007","journal-title":"Reprod Domest Anim."},{"key":"B69","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.livsci.2012.05.011","article-title":"Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders","volume":"149","author":"Crespilho","year":"2012","journal-title":"Livest Sci."},{"key":"B70","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.anireprosci.2010.04.005","article-title":"Effects of extender and equilibration time on post-thaw motility and membrane integrity of cryopreserved Gyr bull semen evaluated by CASA and flow cytometry","volume":"120","author":"Leite","year":"2010","journal-title":"Anim Reprod Sci."},{"key":"B71","doi-asserted-by":"publisher","first-page":"e13063","DOI":"10.1111\/and.13063","article-title":"Comparison of Tris egg yolk-based, Triladyl \u00ae and Optixell \u00ae extender on post-thaw quality, Kinematics and in vivo fertility of Nili Ravi Buffalo (Bubalus bubalis) bull spermatozoa","volume":"50","author":"Naz","year":"2018","journal-title":"Andrologia."},{"key":"B72","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/j.cryobiol.2010.06.002","article-title":"Membrane permeability parameters for freezing of stallion sperm as determined by Fourier transform infrared spectroscopy","volume":"61","author":"Oldenhof","year":"2010","journal-title":"Cryobiology."},{"key":"B73","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1007\/s10561-008-9081-4","article-title":"Cryopreservation of domestic animal sperm cells","volume":"10","author":"Barbas","year":"2009","journal-title":"Cell Tissue Bank."},{"key":"B74","doi-asserted-by":"publisher","first-page":"422","DOI":"10.1089\/rej.2014.1656","article-title":"Cryoprotectant toxicity: facts, issues, and questions","volume":"18","author":"Best","year":"2015","journal-title":"Rejuvenat Res."},{"key":"B75","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/j.smallrumres.2005.02.015","article-title":"A review on goat sperm cryopreservation","volume":"63","author":"Purdy","year":"2006","journal-title":"Small Rumin Res."},{"key":"B76","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/S0378-4320(00)00153-6","article-title":"Storage of bovine semen in liquid and frozen state","volume":"62","author":"Vishwanath","year":"2000","journal-title":"Anim Reprod Sci."},{"key":"B77","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1016\/0093-691X(84)90490-4","article-title":"Effect of cold shock and freezing on loss of total lipids and phospholipids of buffalo spermatozoa (Bubalusbubalis)","volume":"22","author":"Sarmah","year":"1984","journal-title":"Theriogenology."},{"key":"B78","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1111\/j.1439-0531.2011.01806.x","article-title":"Effect of linoleic acid albumin in a dilution solution and long-term equilibration for freezing of bovine spermatozoa with poor freezability","volume":"47","author":"Takahashi","year":"2012","journal-title":"Reprod Domest Anim."},{"key":"B79","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.anireprosci.2017.03.024","article-title":"Effect of different concentrations of egg yolk and virgin coconut oil in Tris-based extenders on chilled and frozen-thawed bull semen","volume":"182","author":"Tarig","year":"2017","journal-title":"Anim Reprod Sci."},{"key":"B80","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.anireprosci.2014.12.001","article-title":"\u03b1-Linolenic acid supplementation in BioXcell\u00ae extender can improve the quality of post-cooling and frozen-thawed bovine sperm","volume":"153","author":"Kaka","year":"2015","journal-title":"Anim Reprod Sci."},{"key":"B81","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1111\/rda.12239","article-title":"Arachidic acid in extender improves post-thaw parameters of cryopreserved nili-ravi buffalo bull semen","volume":"49","author":"Ejaz","year":"2014","journal-title":"Reprod Domest Anim."},{"key":"B82","doi-asserted-by":"publisher","first-page":"1425","DOI":"10.1016\/j.theriogenology.2009.01.019","article-title":"Protective effects of iodixanol during bovine sperm cryopreservation","volume":"71","author":"Saragusty","year":"2009","journal-title":"Theriogenology."},{"key":"B83","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1186\/1477-7827-2-12","article-title":"Reactive oxygen species and sperm cells","volume":"2","author":"Sanocka","year":"2004","journal-title":"Reprod Biol Endocrinol."},{"key":"B84","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1016\/j.repbio.2012.10.001","article-title":"Glutathione-supplemented tris-citric acid extender improves the post-thaw quality and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa","volume":"12","author":"Ansari","year":"2012","journal-title":"Reprod Biol."},{"key":"B85","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1556\/AVet.50.2002.2.13","article-title":"Comparative evaluation of the effect of antioxidants in the conservation of ram semen","volume":"50","author":"Sarl\u00f3s","year":"2002","journal-title":"Acta Vet Hung."},{"key":"B86","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/S0378-4320(03)00049-6","article-title":"Antioxidant supplementation in vitro improves boar sperm motility and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate","volume":"78","author":"Pe\u00f1a","year":"2003","journal-title":"Anim Reprod Sci."},{"key":"B87","doi-asserted-by":"publisher","first-page":"463","DOI":"10.2754\/avb200978030463","article-title":"Influence of various antioxidants on microscopic-oxidative stress indicators and fertilizing ability of frozen-thawed bull semen","volume":"78","author":"Sari\u00f6zkan","year":"2009","journal-title":"Acta Vet Brno."},{"key":"B88","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/j.cryobiol.2010.09.001","article-title":"Effects of antioxidants on post-thawed bovine sperm and oxidative stress parameters: antioxidants protect DNA integrity against cryodamage","volume":"61","author":"Bucak","year":"2010","journal-title":"Cryobiology."},{"key":"B89","doi-asserted-by":"publisher","first-page":"1243","DOI":"10.17582\/journal.pjz\/2017.49.4.1243.1249","article-title":"Effect of green tea extract","volume":"49","author":"Khan","year":"2017","journal-title":"Pakistan J Zool."},{"key":"B90","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/1828051X.2018.1548914","article-title":"Impact of the Spirulina maxima extract addition to semen extender on bovine sperm quality","volume":"18","author":"Mizera","year":"2019","journal-title":"Ital J Anim Sci."},{"key":"B91","doi-asserted-by":"publisher","first-page":"1657","DOI":"10.2527\/jas.2009-2335","article-title":"Effects of trehalose supplementation on semen quality and oxidative stress variables in frozen-thawed bovine semen1","volume":"88","author":"Hu","year":"2010","journal-title":"J Anim Sci."},{"key":"B92","unstructured":"ZubairM AliM AhmadM SajidSM AhmadI GulST Effect of Selenium and Vitamin E on Cryopreservation of Semen and Reproductive Performance of Animals (A Review)2015"},{"key":"B93","doi-asserted-by":"publisher","first-page":"1127","DOI":"10.14202\/vetworld.2014.1127-1131","article-title":"Antioxidative capacity of vitamin E, vitamin C and their combination in cryopreserved Bhadavari bull semen","volume":"7","author":"Mittal","year":"2014","journal-title":"Vet World."},{"key":"B94","unstructured":"AndrabiSMH AnsariM UllahN AfzalMN Effect of Non-Enzymatic Antioxidants in Extender on Post-Thaw Quality of Buffalo (Bubalus Bubalis) Bull Spermatozoa.2008"},{"key":"B95","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/j.aoas.2018.12.001","article-title":"Effect of tonophosphan, zinc oxide, and ascorbic acid on semen, sexual desire, and the fertility rate of Egyptian buffalo bulls","volume":"63","author":"Gabr","year":"2018","journal-title":"Ann Agric Sci."},{"key":"B96","unstructured":"DasS VasudevanD 17133738Protective Effects of Silymarin, a Milk Thistle (Silybium Marianum) Derivative on Ethanol-Induced Oxidative Stress In Liver.2006"},{"key":"B97","doi-asserted-by":"publisher","first-page":"81","DOI":"10.12980\/apjr.6.20170206","article-title":"Impact of silymarin enriched semen extender on bull sperm preservability","volume":"6","author":"El-Sheshtawy","year":"2017","journal-title":"Asian Pacific J Reprod."},{"key":"B98","doi-asserted-by":"publisher","first-page":"98","DOI":"10.5424\/sjar\/2014121-4486","article-title":"Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation","volume":"12","author":"Daghigh-Kia","year":"2014","journal-title":"Spanish J Agric Res."},{"key":"B99","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1016\/S0308-8146(03)00060-8","article-title":"Chemical and structural characterisation of low-density lipoproteins purified from hen egg yolk","volume":"83","author":"Anton","year":"2003","journal-title":"Food Chem."},{"key":"B100","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/S0378-4320(00)00152-4","article-title":"Basic aspects of frozen storage of semen","volume":"62","author":"Holt","year":"2000","journal-title":"Anim Reprod Sci."},{"key":"B101","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/S2305-0500(14)60053-1","article-title":"Effects of different concentrations of sucrose or trehalose on the post-thawing quality of cattle bull semen","volume":"4","author":"El-Sheshtawy","year":"2015","journal-title":"Asian Pacific J. Reprod."},{"key":"B102","doi-asserted-by":"publisher","first-page":"1326","DOI":"10.1016\/j.theriogenology.2008.12.023","article-title":"Butylated hydroxytoluene inclusion in semen extender improves the post-thawed semen quality of Nili-Ravi buffalo (Bubalus bubalis)","volume":"71","author":"Ijaz","year":"2009","journal-title":"Theriogenology."},{"key":"B103","doi-asserted-by":"publisher","first-page":"1401","DOI":"10.1095\/biolreprod57.6.1401","article-title":"Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa1","volume":"57","author":"Garner","year":"1997","journal-title":"Biol Reprod."},{"key":"B104","first-page":"1","article-title":"in vitro assessment of viability and fertilizing capacity of bull spermatozoa","volume":"43","author":"Rodriguez-Martinez","year":"1997","journal-title":"J Reprod Dev."},{"key":"B105","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1530\/rep.1.00549","article-title":"Cytoskeleton localization in the sperm head prior to fertilization","volume":"130","author":"Dvor\u00e1kov\u00e1","year":"2005","journal-title":"Reproduction"},{"key":"B106","doi-asserted-by":"publisher","first-page":"672","DOI":"10.1095\/biolreprod65.3.672","article-title":"Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail","volume":"65","author":"Peknicova","year":"2001","journal-title":"Biol Reprod."},{"key":"B107","doi-asserted-by":"publisher","first-page":"jcs","DOI":"10.1242\/jcs.218958","article-title":"Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis","volume":"2018","author":"Romarowski","year":"2018","journal-title":"J Cell Sci."},{"key":"B108","doi-asserted-by":"publisher","first-page":"379","DOI":"10.3389\/fendo.2017.00379","article-title":"Olfactory receptors in semen and in the male tract: from proteome to proteins","volume":"23","author":"Milardi","year":"2018","journal-title":"Front. Endocrinol."},{"key":"B109","doi-asserted-by":"publisher","first-page":"229","DOI":"10.12717\/DR.2017.21.3.229","article-title":"A simple confocal microscopy-based method for assessing sperm movement","volume":"21","author":"Kim","year":"2017","journal-title":"Dev Reprod."},{"key":"B110","doi-asserted-by":"publisher","first-page":"17931","DOI":"10.1038\/s41598-017-18123-1","article-title":"Single-cell-based evaluation of sperm progressive motility via fluorescent assessment of mitochondria membrane potential","volume":"7","author":"Moscatelli","year":"2017","journal-title":"Sci Rep."},{"key":"B111","doi-asserted-by":"publisher","first-page":"13599","DOI":"10.1039\/C5CC04731E","article-title":"A red emitting mitochondria-targeted AIE probe as an indicator for membrane potential and mouse sperm activity","volume":"51","author":"Zhao","year":"2015","journal-title":"Chem Commun."},{"key":"B112","doi-asserted-by":"publisher","first-page":"1382","DOI":"10.1016\/j.freeradbiomed.2003.08.010","article-title":"In situ detection and localization of lipid peroxidation in individual bovine sperm cells","volume":"5","author":"Brouwers","year":"2003","journal-title":"Free Radic Biol Med."},{"key":"B113","doi-asserted-by":"publisher","first-page":"1382","DOI":"10.3791\/55323","article-title":"Evaluation of intracellular location of reactive oxygen species in Solea Senegalensis Spermatozoa","volume":"35","author":"Valcarce","year":"2018","journal-title":"J Vis Exp."},{"key":"B114","unstructured":"BozzolaJ RussellL Electron Microscopy: Principles and Techniques for Biologists.1999"},{"key":"B115","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.micron.2012.05.002","article-title":"Ultrastructure of human gametes, fertilization and embryos in assisted reproduction: a personal survey","volume":"44","author":"Sathananthan","year":"2013","journal-title":"Micron."},{"key":"B116","doi-asserted-by":"crossref","DOI":"10.1201\/9781482289343","volume-title":"Electron Microscopy and Analysis.","author":"Goodhew","year":"2000"},{"key":"B117","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1007\/s10815-008-9232-3","article-title":"Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa","volume":"25","author":"Ozkavukcu","year":"2008","journal-title":"J Assist Reprod Genet."},{"key":"B118","doi-asserted-by":"publisher","first-page":"171","DOI":"10.3109\/19396368.2016.1155242","article-title":"The importance of transmission electron microscopy analysis of spermatozoa: Diagnostic applications and basic research","volume":"62","author":"Moretti","year":"2016","journal-title":"Syst Biol Reprod Med."},{"key":"B119","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1017\/S0967199413000026","article-title":"Digital holographic microscopy for the evaluation of human sperm structure","volume":"22","author":"Coppola","year":"2014","journal-title":"Zygote."},{"key":"B120","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1109\/JSTQE.2009.2036741","article-title":"Quantitative label-free animal sperm imaging by means of digital holographic microscopy","volume":"5","author":"Di Caprio","year":"2010","journal-title":"IEEE J Sel Top Quantum Electron."},{"key":"B121","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1117\/12.728262","article-title":"Digital holographic microscopy for live cell applications","volume":"47","author":"Kemper","year":"2008","journal-title":"Appl Opt."},{"key":"B122","doi-asserted-by":"publisher","first-page":"4512","DOI":"10.1039\/c3lc50515d","article-title":"Digital holography as a method for 3D imaging and estimating the biovolume of motile cells","volume":"13","author":"Merola","year":"2013","journal-title":"Lab Chip."},{"key":"B123","doi-asserted-by":"publisher","first-page":"16018","DOI":"10.1073\/pnas.1212506109","article-title":"High-throughput lensfree 3D tracking of human sperms reveals rare statistics of helical trajectories","volume":"109","author":"Su","year":"2012","journal-title":"Proc Natl Acad Sci USA."},{"key":"B124","doi-asserted-by":"publisher","first-page":"17121","DOI":"10.1038\/lsa.2017.121","article-title":"Label-free 3D computational imaging of spermatozoon locomotion, head spin and flagellum beating over a large volume","volume":"7","author":"Daloglu","year":"2018","journal-title":"Light Sci Appl."},{"key":"B125","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1002\/jbio.200910012","article-title":"Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells","volume":"2","author":"Huser","year":"2009","journal-title":"J Biophotonics"},{"key":"B126","doi-asserted-by":"publisher","first-page":"1641","DOI":"10.1093\/humrep\/der122","article-title":"In situ visualization of damaged DNA in human sperm by Raman microspectroscopy","volume":"26","author":"Mallidis","year":"2011","journal-title":"Hum Reprod."},{"key":"B127","doi-asserted-by":"crossref","DOI":"10.1109\/AEIT.2015.7415259","article-title":"Analysis of bovine sperm cells by a combined holographic and Raman microscopy approach","volume-title":"2015 AEIT International Annual Conference","author":"De Angelis","year":"2015"},{"key":"B128","doi-asserted-by":"publisher","first-page":"141","DOI":"10.3390\/bios5020141","article-title":"Label-free imaging and biochemical characterization of bovine sperm cells","volume":"5","author":"Ferrara","year":"2015","journal-title":"Biosensors"},{"key":"B129","doi-asserted-by":"publisher","first-page":"1690","DOI":"10.1364\/BOE.5.001690","article-title":"Confocal Raman micro-spectroscopy for rapid and label-free detection of maleic acid-induced variations in human sperm","volume":"5","author":"Li","year":"2014","journal-title":"Biomed Opt Express"},{"key":"B130","doi-asserted-by":"publisher","first-page":"10424","DOI":"10.1039\/C4RA12158A","article-title":"Non-invasive chemical assessment of living human spermatozoa","volume":"5","author":"Edengeiser","year":"2015","journal-title":"RSC Adv."},{"key":"B131","doi-asserted-by":"publisher","first-page":"9876063","DOI":"10.1155\/2017\/9876063","article-title":"Combined raman spectroscopy and digital holographic microscopy for sperm cell quality analysis","volume":"2017","author":"De Angelis","year":"2017","journal-title":"J Spectrosc."},{"key":"B132","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1109\/JSTQE.2015.2496265","article-title":"Simultaneous holographic microscopy and Raman spectroscopy monitoring of human spermatozoa photodegradation","volume":"22","author":"Ferrara","year":"2016","journal-title":"IEEE J Sel Top Quant Electr."},{"key":"B133","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1071\/RD07037","article-title":"Applications and interpretation of computer-assisted sperm analyses and sperm sorting methods in assisted breeding and comparative research","volume":"19","author":"Holt","year":"2007","journal-title":"Reprod Fertil Dev."},{"key":"B134","doi-asserted-by":"publisher","first-page":"1973","DOI":"10.1016\/S0093-691X(02)01297-9","article-title":"Identification of sperm subpopulations with specific motility characteristics in stallion ejaculates","volume":"59","author":"Quintero-Moreno","year":"2003","journal-title":"Theriogenology."},{"key":"B135","doi-asserted-by":"publisher","first-page":"e1006691","DOI":"10.1371\/journal.pcbi.1006691","article-title":"OpenCASA: a new open-source and scalable tool for sperm quality analysis","volume":"15","author":"Alqu\u00e9zar-Baeta","year":"2019","journal-title":"PLoS Comput Biol"},{"key":"B136","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1016\/j.theriogenology.2013.09.004","article-title":"Computer-assisted sperm analysis (CASA): capabilities and potential developments","volume":"81","author":"Amann","year":"2014","journal-title":"Theriogenology."},{"key":"B137","first-page":"1","volume-title":"Flow Cytometry: An Introduction.","author":"Givan","year":"2011"},{"key":"B138","doi-asserted-by":"publisher","first-page":"1584","DOI":"10.1002\/eji.201646632","article-title":"Guidelines for the use of flow cytometry and cell sorting in immunological studies","volume":"47","author":"Frenette","year":"2017","journal-title":"Eur J Immunol."},{"key":"B139","doi-asserted-by":"publisher","first-page":"1934","DOI":"10.1073\/pnas.69.7.1934","article-title":"Demonstration that antigen-binding cells are precursors of antibody-producing cells after purification with a fluorescence-activated cell sorter","volume":"69","author":"Julius","year":"1972","journal-title":"Proc Natl Acad Sci USA."},{"key":"B140","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1016\/j.theriogenology.2016.02.007","article-title":"Effects of cryopreservation on sperm viability, synthesis of reactive oxygen species, and DNA damage of bovine sperm","volume":"86","author":"G\u00fcrler","year":"2015","journal-title":"Theriogenology."},{"key":"B141","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1016\/0093-691X(94)00065-3","article-title":"Assessment of boar sperm viability using a combination of two fluorophores","volume":"43","author":"Althouse","year":"1995","journal-title":"Theriogenology"},{"key":"B142","first-page":"209","article-title":"Flow cytometric analysis of viability of bull sperm cells","volume":"19","author":"Matyus","year":"1984","journal-title":"Acta Biochim Biophys Acad Sci Hung."},{"key":"B143","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/S0378-4320(02)00247-6","article-title":"Evaluation of cryopreserved stallion semen from Tori and Estonian breeds using CASA and flow cytometry","volume":"76","author":"Kavak","year":"2003","journal-title":"Anim Reprod Sci."},{"key":"B144","doi-asserted-by":"publisher","first-page":"1343","DOI":"10.1083\/jcb.99.4.1343","article-title":"Sperm surface proteins persist after fertilization","volume":"99","author":"Gundersen","year":"1984","journal-title":"J Cell Biol."},{"key":"B145","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1111\/j.1439-0531.2010.01622.x","article-title":"Probes and techniques for sperm evaluation by flow cytometry","volume":"45","author":"Mart\u00ednez-Pastor","year":"2010","journal-title":"Reprod Domest Anim."},{"key":"B146","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1016\/j.theriogenology.2004.09.024","article-title":"Flow cytometric evaluation of sperm parameters in relation to fertility potential","volume":"63","author":"Gillan","year":"2005","journal-title":"Theriogenology."},{"key":"B147","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1095\/biolreprod53.2.276","article-title":"Dual DNA staining assessment of bovine sperm viability using SYBR-14 and propidium iodide","volume":"53","author":"Garner","year":"1994","journal-title":"J Androl."},{"key":"B148","doi-asserted-by":"publisher","first-page":"1828","DOI":"10.1095\/biolreprod.102.011445","article-title":"A triple-stain flow cytometric method to assess plasma- and acrosome-membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles1","volume":"68","author":"Nagy","year":"2004","journal-title":"Biol Reprod."},{"key":"B149","doi-asserted-by":"publisher","first-page":"1642","DOI":"10.1016\/j.theriogenology.2011.12.009","article-title":"Flow cytometry as an estimation tool for honey bee sperm viability","volume":"77","author":"Rzymski","year":"2012","journal-title":"Theriogenology."},{"key":"B150","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1002\/cyto.b.21506","article-title":"A new method for evaluating stallion sperm viability and mitochondrial membrane potential in fixed semen samples","volume":"94","author":"Pe\u00f1a","year":"2018","journal-title":"Cytom Part B Clin Cytom."},{"key":"B151","doi-asserted-by":"publisher","first-page":"5824","DOI":"10.3168\/jds.2016-12484","article-title":"Sperm viability, reactive oxygen species, and DNA fragmentation index combined can discriminate between above- and below-average fertility bulls","volume":"100","author":"Kumaresan","year":"2017","journal-title":"J Dairy Sci."},{"key":"B152","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1186\/s13028-018-0396-2","article-title":"Sperm quality in frozen beef and dairy bull semen","volume":"60","author":"Morrell","year":"2018","journal-title":"Acta Vet Scand."},{"key":"B153","doi-asserted-by":"publisher","first-page":"6149","DOI":"10.1038\/s41598-018-24269-3","article-title":"Cryopreservation of fish spermatogonial cells: the future of natural history collections","volume":"8","author":"Hagedorn","year":"2018","journal-title":"Sci Rep."},{"key":"B154","doi-asserted-by":"publisher","first-page":"e0202514","DOI":"10.1371\/journal.pone.0202514","article-title":"Cryopreservation effects on a viable sperm sterlet (Acipenser ruthenus) subpopulation obtained by a Percoll density gradient method","volume":"13","author":"Horokhovatskyi","year":"2018","journal-title":"PLoS ONE."},{"key":"B155","first-page":"666","article-title":"Two-color, fluorescence-based microplate assay for apoptosis detection","volume":"32","author":"Wronski","year":"2002","journal-title":"Biotechniques"},{"key":"B156","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/j.anireprosci.2005.12.014","article-title":"Detection of early changes in sperm membrane integrity pre-freezing can estimate post-thaw quality of boar spermatozoa","volume":"144","author":"Pe\u00f1a","year":"2007","journal-title":"Anim Reprod Sci."},{"key":"B157","doi-asserted-by":"publisher","first-page":"e30688","DOI":"10.1371\/journal.pone.0030688","article-title":"Autophagy and apoptosis have a role in the survival or death of stallion spermatozoa during conservation in refrigeration","volume":"7","author":"Gallardo Bola\u00f1os","year":"2012","journal-title":"PLoS ONE"},{"key":"B158","doi-asserted-by":"publisher","first-page":"947","DOI":"10.1016\/S0093-691X(01)00456-3","article-title":"Assessment of sperm quality through fluorometry and sperm chromatin structure assay in relation to field fertility of frozen-thawed semen from Swedish AI bulls","volume":"55","author":"Januskauskas","year":"2001","journal-title":"Theriogenology."},{"key":"B159","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.anireprosci.2017.12.009","article-title":"A simple flow cytometry protocol to determine simultaneously live, dead and apoptotic stallion spermatozoa in fresh and frozen thawed samples","volume":"189","author":"Gil","year":"2018","journal-title":"Anim Reprod Sci."},{"key":"B160","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1016\/S0378-4320(01)00160-9","article-title":"Assessment of sperm quality: a flow cytometric approach","volume":"68","author":"Graham","year":"2001","journal-title":"Anim Reprod Sci."},{"key":"B161","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1095\/biolreprod41.4.635","article-title":"Methods for evaluating the acrosomal status of mammalian sperm1","volume":"41","author":"Cross","year":"2005","journal-title":"Biol Reprod."},{"key":"B162","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/B978-012311629-1\/50005-X","article-title":"Capacitation","volume-title":"Fertilization","author":"Jaiswal","year":"2002"},{"key":"B163","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1530\/jrf.0.0810127","article-title":"Specific labelling by peanut agglutinin of the outer acrosomal membrane of the human spermatozoon","volume":"81","author":"Mortimer","year":"1987","journal-title":"Reproduction"},{"key":"B164","doi-asserted-by":"publisher","first-page":"999","DOI":"10.14670\/HH-24.999","article-title":"Improved methodology for the detection and quantification of the acrosome reaction in mouse spermatozoa","volume":"2","author":"Lybaert","year":"2009","journal-title":"Histol Histopathol."},{"key":"B165","doi-asserted-by":"publisher","first-page":"1111","DOI":"10.1016\/j.theriogenology.2011.10.013","article-title":"Sperm concentration at freezing affects post-thaw quality and fertility of ram semen","volume":"77","author":"Alvarez","year":"2012","journal-title":"Theriogenology."},{"key":"B166","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1007\/978-1-62703-038-0_16","article-title":"Flow cytometric methods for sperm assessment","volume":"927","author":"Robles","year":"2013","journal-title":"Methods Mol Biol."},{"key":"B167","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1136\/vr.103897","article-title":"Effects of season on bull sperm quality in thawed samples in northern Spain","volume":"180","author":"Sab\u00e9s-Alsina","year":"2017","journal-title":"Vet Rec."},{"key":"B168","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1177\/30.3.6174566","article-title":"Simultaneous measurement by flow cytometry of sperm cell viability and mitochondrial membrane potential related to cell motility","volume":"30","author":"Evenson","year":"1982","journal-title":"J Histochem Cytochem."},{"key":"B169","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1111\/j.1439-0531.2008.01211.x","article-title":"Mitochondria in mammalian sperm physiology and pathology: a review","volume":"44","author":"Pe\u00f1a","year":"2009","journal-title":"Reprod Domest Anim."},{"key":"B170","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1093\/humrep\/del399","article-title":"Kinetics of occurrence of some features of apoptosis during the cryopreservation process of bovine spermatozoa","volume":"22","author":"Martin","year":"2007","journal-title":"Hum Reprod."},{"key":"B171","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1002\/cyto.a.21061","article-title":"Cytometric assessment of mitochondria using fluorescent probes","volume":"79","author":"Cottet-Rousselle","year":"2011","journal-title":"Cytom Part A"},{"key":"B172","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/S0014-5793(97)00669-8","article-title":"JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess \u0394\u03a8 changes in intact cells: implications for studies on mitochondrial functionality during apoptosis","volume":"411","author":"Salvioli","year":"1997","journal-title":"FEBS Lett."},{"key":"B173","doi-asserted-by":"publisher","first-page":"990","DOI":"10.1101\/pdb.prot5648","article-title":"Labeling mitochondria with mitotracker dyes","volume":"2011","author":"Chazotte","year":"2011","journal-title":"Cold Spring Harb Protoc."},{"key":"B174","doi-asserted-by":"publisher","first-page":"2311","DOI":"10.1016\/j.theriogenology.2004.10.010","article-title":"Mitochondrial activity of frozen-thawed spermatozoa assessed by MitoTracker Deep Red 633","volume":"63","author":"Hallap","year":"2005","journal-title":"Theriogenology."},{"key":"B175","doi-asserted-by":"publisher","first-page":"e18112","DOI":"10.1371\/journal.pone.0018112","article-title":"Not all sperm are equal: functional mitochondria characterize a subpopulation of human sperm with better fertilization potential","volume":"6","author":"Sousa","year":"2011","journal-title":"PLoS ONE."},{"key":"B176","doi-asserted-by":"publisher","first-page":"1691","DOI":"10.1016\/S0093-691X(00)00308-3","article-title":"Assessment of equine sperm mitochondrial function using JC-1","volume":"53","author":"Gravance","year":"2000","journal-title":"Theriogenology."},{"key":"B177","doi-asserted-by":"publisher","first-page":"e0168823","DOI":"10.1371\/journal.pone.0168823","article-title":"Mitochondrial biomarkers reflect semen quality: results from the MARCHS study in chongqing, China","volume":"11","author":"Zhang","year":"2016","journal-title":"PLoS ONE."},{"key":"B178","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.anireprosci.2017.12.007","article-title":"Factors affecting staining to discriminate between bull sperm with greater and lesser mitochondrial membrane potential","volume":"189","author":"Gloria","year":"2018","journal-title":"Anim Reprod Sci."},{"key":"B179","doi-asserted-by":"publisher","first-page":"1","DOI":"10.5534\/wjmh.2014.32.1.1","article-title":"Effect of oxidative stress on male reproduction","volume":"32","author":"Agarwal","year":"2014","journal-title":"World J Mens Health"},{"key":"B180","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1016\/j.smallrumres.2007.09.002","article-title":"Effect of anti-oxidants and oxidative stress parameters on ram semen after the freeze-thawing process","volume":"75","author":"Bucak","year":"2008","journal-title":"Small Rumin Res."},{"key":"B181","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.anireprosci.2005.11.002","article-title":"A comparative study on lipid peroxidation, activities of antioxidant enzymes and viability of cattle and buffalo bull spermatozoa during storage at refrigeration temperature","volume":"96","author":"Nair","year":"2006","journal-title":"Anim Reprod Sci."},{"key":"B182","doi-asserted-by":"publisher","first-page":"2089","DOI":"10.2527\/jas.2005-766","article-title":"Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry","volume":"84","author":"Guthrie","year":"2006","journal-title":"J Anim Sci."},{"key":"B183","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1016\/S0891-5849(99)00107-0","article-title":"Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader","volume":"27","author":"Wang","year":"1999","journal-title":"Free Radic Biol Med."},{"key":"B184","doi-asserted-by":"publisher","first-page":"995","DOI":"10.1016\/j.freeradbiomed.2007.06.026","article-title":"Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects","volume":"43","author":"Wardman","year":"2007","journal-title":"Free Radic Biol Med."},{"key":"B185","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1038\/nprot.2007.473","article-title":"Detection of 2-hydroxyethidium in cellular systems: a unique marker product of superoxide and hydroethidine","volume":"3","author":"Zielonka","year":"2008","journal-title":"Nat Protoc."},{"key":"B186","doi-asserted-by":"publisher","first-page":"1968","DOI":"10.1210\/jc.2005-2711","article-title":"Definitive evidence for the nonmitochondrial production of superoxide anion by human spermatozoa","volume":"91","author":"De Iuliis","year":"2006","journal-title":"J Clin Endocrinol Metab."},{"key":"B187","doi-asserted-by":"publisher","first-page":"819","DOI":"10.1111\/rda.12984","article-title":"Detection of intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) and lipid peroxidation during cryopreservation of alpaca spermatozoa","volume":"52","author":"Evangelista-Vargas","year":"2017","journal-title":"Reprod Domest Anim."},{"key":"B188","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.theriogenology.2017.10.035","article-title":"Seminal plasma antioxidants are directly involved in boar sperm cryotolerance","volume":"107","author":"Li","year":"2018","journal-title":"Theriogenology."},{"key":"B189","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.freeradbiomed.2011.09.030","article-title":"Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations","volume":"52","author":"Kalyanaraman","year":"2012","journal-title":"Free Radic Biol Med."},{"key":"B190","doi-asserted-by":"publisher","first-page":"4447","DOI":"10.3168\/jds.2012-6484","article-title":"Reducing sperm concentration is critical to limiting the oxidative stress challenge in liquid bull semen","volume":"96","author":"Murphy","year":"2013","journal-title":"J Dairy Sci."},{"key":"B191","doi-asserted-by":"publisher","first-page":"3199","DOI":"10.1210\/jc.2007-2616","article-title":"Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa","volume":"93","author":"Koppers","year":"2008","journal-title":"J Clin Endocrinol Metab."},{"key":"B192","doi-asserted-by":"publisher","first-page":"698","DOI":"10.1111\/j.2047-2927.2012.00056.x","article-title":"On methods for the detection of reactive oxygen species generation by human spermatozoa: analysis of the cellular responses to catechol oestrogen, lipid aldehyde, menadione and arachidonic acid","volume":"1","author":"Aitken","year":"2013","journal-title":"Andrology."},{"key":"B193","doi-asserted-by":"publisher","first-page":"947","DOI":"10.1071\/RD12204","article-title":"The effect of the in vitro supplementation of exogenous long-chain fatty acids on bovine sperm cell function","volume":"25","author":"Kiernan","year":"2013","journal-title":"Reprod Fertil Dev."},{"key":"B194","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1023\/A:1009844109023","article-title":"Sperm chromatin structure assay is useful for fertility assessment Donald","volume":"22","author":"Evenson","year":"2000","journal-title":"Methods Cell Sci."},{"key":"B195","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.fertnstert.2006.05.057","article-title":"Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome","volume":"87","author":"Benchaib","year":"2007","journal-title":"Fertil Steril."},{"key":"B196","doi-asserted-by":"publisher","first-page":"1073","DOI":"10.1016\/j.theriogenology.2009.06.027","article-title":"Washing increases the susceptibility to exogenous oxidative stress in red deer spermatozoa","volume":"72","author":"Dom\u00ednguez-Rebolledo","year":"2009","journal-title":"Theriogenology."},{"key":"B197","doi-asserted-by":"publisher","first-page":"213","DOI":"10.2527\/1999.77suppl_2213x","article-title":"The Beltsville sperm sexing technology: high-speed sperm sorting gives improved sperm output for in vitro fertilization and AI","volume":"77","author":"Johnson","year":"1999","journal-title":"J Anim Sci."},{"key":"B198","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1002\/j.1939-4640.2001.tb02209.x","article-title":"Sex-sorting mammalian sperm: concept to application in animals","volume":"22","author":"Garner","year":"2001","journal-title":"J Androl."},{"key":"B199","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1530\/reprod\/124.6.733","article-title":"Current status of sexing mammalian spermatozoa","volume":"124","author":"Seidel","year":"2002","journal-title":"Reproduction."}],"container-title":["Frontiers in Veterinary Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fvets.2019.00268\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T12:01:57Z","timestamp":1721649717000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fvets.2019.00268\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,27]]},"references-count":199,"alternative-id":["10.3389\/fvets.2019.00268"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2019.00268","relation":{},"ISSN":["2297-1769"],"issn-type":[{"value":"2297-1769","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,27]]},"article-number":"268"}}