{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T13:18:54Z","timestamp":1762607934537,"version":"3.41.2"},"reference-count":30,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,7,17]],"date-time":"2020-07-17T00:00:00Z","timestamp":1594944000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31772730","31972700"],"award-info":[{"award-number":["31772730","31972700"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2015CB150300"],"award-info":[{"award-number":["2015CB150300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"DOI":"10.3389\/fvets.2020.00357","type":"journal-article","created":{"date-parts":[[2020,7,17]],"date-time":"2020-07-17T09:38:36Z","timestamp":1594978716000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Characterization of Neospora Caninum Microneme Protein 26 and Its Potential Use as a Diagnostic Marker for Neosporosis in Cattle"],"prefix":"10.3389","volume":"7","author":[{"given":"Xianmei","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingju","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2020,7,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1473","DOI":"10.1016\/S0020-7519(98)00138-6","article-title":"Dogs are definitive hosts of Neospora caninum","volume":"28","author":"McAllister","year":"1998","journal-title":"Int J Parasitol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.vetpar.2011.05.031","article-title":"Neosporosis in animals\u2013the last five years","volume":"180","author":"Dubey","year":"2011","journal-title":"Vet Parasitol."},{"key":"B3","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.2460\/javma.1999.215.10.1485","article-title":"Outbreak of abortion associated with Neospora caninum infection in a beef herd","volume":"215","author":"Waldner","year":"1999","journal-title":"J Am Vet Med Assoc."},{"key":"B4","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/j.vetpar.2003.10.010","article-title":"Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAG1","volume":"118","author":"Chahan","year":"2003","journal-title":"Vet Parasitol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1016\/j.vetpar.2006.08.034","article-title":"Serodiagnosis of Neospora caninum infection in cattle using a recombinant tNcSRS2 protein-based ELISA. Vet Parasitol","volume":"143","author":"Liu","year":"2007"},{"key":"B6","doi-asserted-by":"publisher","first-page":"2117","DOI":"10.1007\/s00436-013-3309-3","article-title":"Neospora caninum surface antigen (p40) is a potential diagnostic marker for cattle neosporosis","volume":"112","author":"He","year":"2013","journal-title":"Parasitol Res."},{"key":"B7","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.1007\/s00436-011-2286-7","article-title":"Immunoblot diagnosis of infection with Neospora caninum in cattle based on recombinant NcSAG4 Antigen","volume":"108","author":"Hu","year":"2011","journal-title":"Parasitol Res."},{"key":"B8","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1017\/S0031182012002041","article-title":"Specific antibody responses against Neospora caninum recombinant rNcGRA7, rNcSAG4, rNcBSR4 and rNcSRS9 proteins are correlated with virulence in mice","volume":"140","author":"Jimenez-Ruiz","year":"2013","journal-title":"Parasitology."},{"key":"B9","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1016\/j.exppara.2015.07.003","article-title":"Evaluation of Neospora caninum truncated dense granule protein 2 for serodiagnosis by enzyme-linked immunosorbent assay in dogs","volume":"157","author":"Jin","year":"2015","journal-title":"Exp Parasitol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/S0166-6851(98)00031-0","article-title":"Isolation of the cDNA encoding a dense granule associated antigen (NCDG2) of Neospora caninum","volume":"93","author":"Liddell","year":"1998","journal-title":"Mol Biochem Parasitol."},{"key":"B11","first-page":"69","article-title":"Development of an indirect ELISA using different fragments of recombinant Ncgra7 for detection of Neospora caninum infection in cattle and water buffalo","volume":"10","author":"Hamidinejat","year":"2015","journal-title":"Iran J Parasitol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/j.vetpar.2012.01.003","article-title":"Characterization of Neospora caninum microneme protein 10 (NcMIC10) and its potential use as a diagnostic marker for neosporosis","volume":"187","author":"Yin","year":"2012","journal-title":"Vet Parasitol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1128\/CVI.05669-11","article-title":"Enzyme-linked immunosorbent assays based on Neospora caninum dense granule protein 7 and profilin for estimating the stage of neosporosis","volume":"19","author":"Hiasa","year":"2012","journal-title":"Clin Vaccine Immunol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.vetpar.2013.07.033","article-title":"Serological diagnosis of bovine neosporosis: a comparative study of commercially available ELISA tests","volume":"198","author":"Alvarez-Garcia","year":"2013","journal-title":"Vet Parasitol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1111\/j.1462-5822.2011.01585.x","article-title":"Invasion factors are coupled to key signalling events leading to the establishment of infection in apicomplexan parasites","volume":"13","author":"Santos","year":"2011","journal-title":"Cell Microbiol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1186\/1756-3305-6-335","article-title":"Unravelling the Neospora caninum secretome through the secreted fraction (ESA) and quantification of the discharged tachyzoite using high-resolution mass spectrometry-based proteomics","volume":"6","author":"Pollo-Oliveira","year":"2013","journal-title":"Parasit Vectors."},{"key":"B17","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1016\/j.cimid.2014.10.003","article-title":"Excretory-secretory antigens: a suitable candidate for immunization against ocular toxoplasmosis in a murine model","volume":"37","author":"Norouzpour Deilami","year":"2014","journal-title":"Comp Immunol Microbiol Infect Dis."},{"key":"B18","doi-asserted-by":"publisher","first-page":"3791","DOI":"10.1007\/s00436-015-4609-6","article-title":"MIC3, a novel cross-protective antigen expressed in Toxoplasma gondii and Neospora caninum","volume":"114","author":"Yang","year":"2015","journal-title":"Parasitol Res."},{"key":"B19","doi-asserted-by":"publisher","first-page":"e1002567","DOI":"10.1371\/journal.ppat.1002567","article-title":"Comparative genomics of the apicomplexan parasites Toxoplasma gondii and Neospora caninum: Coccidia differing in host range and transmission strategy","volume":"8","author":"Reid","year":"2012","journal-title":"PLos Pathog."},{"key":"B20","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/j.vetpar.2018.03.018","article-title":"NcGRA17 is an important regulator of parasitophorous vacuole morphology and pathogenicity of Neospora caninum","volume":"264","author":"Yang","year":"2018","journal-title":"Vet Parasitol."},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-12-19","article-title":"Development of a diagnostic method for neosporosis in cattle using recombinant Neospora caninum proteins","author":"Dong","year":"2012","journal-title":"BMC Biotechnol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"2893","DOI":"10.1007\/s00436-015-4490-3","article-title":"Identification and characterization of a microneme protein (NcMIC6) in Neospora caninum","volume":"114","author":"Li","year":"2015","journal-title":"Parasitol Res."},{"key":"B23","first-page":"1269","article-title":"Newly recognized fatal protozoan disease of dogs","volume":"192","author":"Dubey","year":"1988","journal-title":"J Am Vet Med Assoc."},{"key":"B24","doi-asserted-by":"publisher","first-page":"899","DOI":"10.1007\/s00436-011-2332-5","article-title":"Identification of the cross-reactive and species-specific antigens between Neospora caninum and Toxoplasma gondii tachyzoites by a proteomics approach","volume":"109","author":"Zhang","year":"2011","journal-title":"Parasitol Res."},{"key":"B25","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.molbiopara.2006.11.005","article-title":"Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites","volume":"151","author":"Zhang","year":"2007","journal-title":"Mol Biochem Parasitol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"528","DOI":"10.1016\/j.tcb.2004.08.002","article-title":"The glideosome: a molecular machine powering motility and host-cell invasion by Apicomplexa","volume":"14","author":"Keeley","year":"2004","journal-title":"Trends Cell Biol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/S0166-6851(99)00228-5","article-title":"Molecular characterization of a thrombospondin-related anonymous protein homologue in Neospora caninum","volume":"107","author":"Lovett","year":"2000","journal-title":"Mol Biochem Parasitol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1177\/104063879300500412","article-title":"Detection of serum antibody responses in cattle with natural or experimental Neospora infections","volume":"5","author":"Conrad","year":"1993","journal-title":"J Vet Diagn Invest."},{"key":"B29","doi-asserted-by":"publisher","first-page":"753","DOI":"10.2307\/3284583","article-title":"Differential expression of cell surface- and dense granule-associated Neospora caninum proteins in tachyzoites and bradyzoites","volume":"84","author":"Fuchs","year":"1998","journal-title":"J Parasitol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.vetpar.2003.12.010","article-title":"Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines","volume":"120","author":"Blumr\u00f6der","year":"2004","journal-title":"Vet Parasitol."}],"container-title":["Frontiers in Veterinary Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fvets.2020.00357\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,10]],"date-time":"2024-08-10T06:36:24Z","timestamp":1723271784000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fvets.2020.00357\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,17]]},"references-count":30,"alternative-id":["10.3389\/fvets.2020.00357"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2020.00357","relation":{},"ISSN":["2297-1769"],"issn-type":[{"type":"electronic","value":"2297-1769"}],"subject":[],"published":{"date-parts":[[2020,7,17]]},"article-number":"357"}}