{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:44:27Z","timestamp":1791287067352,"version":"4.1.0"},"reference-count":96,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T00:00:00Z","timestamp":1615334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000277","name":"Department for Environment, Food and Rural Affairs, UK Government","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000277","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["EVAg, grant no. 653316"],"award-info":[{"award-number":["EVAg, grant no. 653316"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010785","name":"Canada First Research Excellence Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010785","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>There are ~240 species of Culicidae in Mexico, of which some are vectors of arthropod-borne viruses such as Zika virus, dengue virus, chikungunya virus, and West Nile virus. Thus, the identification of mosquito feeding preferences is paramount to understanding of vector\u2013host\u2013pathogen interactions that, in turn, can aid the control of disease outbreaks. Typically, DNA and RNA are extracted separately for animal (insects and blood meal hosts) and viral identification, but this study demonstrates that multiple organisms can be analyzed from a single RNA extract. For the first time, residual DNA present in standard RNA extracts was analyzed by DNA barcoding in concert with Sanger and next-generation sequencing (NGS) to identify both the mosquito species and the source of their meals in blood-fed females caught in seven sylvan communities in Chiapas State, Mexico. While mosquito molecular identification involved standard barcoding methods, the sensitivity of blood meal identification was maximized by employing short primers with NGS. In total, we collected 1,634 specimens belonging to 14 genera, 25 subgenera, and 61 morphospecies of mosquitoes. Of these, four species were new records for Mexico (<jats:italic>Aedes guatemala, Ae. insolitus, Limatus asulleptus, Trichoprosopon pallidiventer<\/jats:italic>), and nine were new records for Chiapas State. DNA barcode sequences for &amp;gt;300 bp of the COI gene were obtained from 291 specimens, whereas 130 bp sequences were recovered from another 179 specimens. High intraspecific divergence values (&amp;gt;2%) suggesting cryptic species complexes were observed in nine taxa:<jats:italic>Anopheles eiseni<\/jats:italic>(5.39%),<jats:italic>An. pseudopunctipennis<\/jats:italic>(2.79%),<jats:italic>Ae. podographicus<\/jats:italic>(4.05%),<jats:italic>Culex eastor<\/jats:italic>(4.88%),<jats:italic>Cx. erraticus<\/jats:italic>(2.28%),<jats:italic>Toxorhynchites haemorrhoidalis<\/jats:italic>(4.30%),<jats:italic>Tr. pallidiventer<\/jats:italic>(4.95%),<jats:italic>Wyeomyia adelpha\/Wy. guatemala<\/jats:italic>(7.30%), and<jats:italic>Wy. pseudopecten<\/jats:italic>(4.04%). The study increased the number of mosquito species known from 128 species to 138 species for Chiapas State, and 239 for Mexico as a whole. Blood meal analysis showed that<jats:italic>Aedes angustivittatus<\/jats:italic>fed on ducks and chicken, whereas<jats:italic>Psorophora albipes<\/jats:italic>fed on humans.<jats:italic>Culex quinquefasciatus<\/jats:italic>fed on diverse hosts including chicken, human, turkey, and Mexican grackle. No arbovirus RNA was detected by reverse transcriptase\u2013polymerase chain reaction in the surveyed specimens. This study demonstrated, for the first time, that residual DNA present in RNA blood meal extracts can be used to identify host vectors, highlighting the important role of molecular approaches in both vector identification and revealing host\u2013vector\u2013pathogen interactions.<\/jats:p>","DOI":"10.3389\/fvets.2020.564791","type":"journal-article","created":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T06:40:11Z","timestamp":1615358411000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":23,"title":["An Integrated Molecular Approach to Untangling Host\u2013Vector\u2013Pathogen Interactions in Mosquitoes (Diptera: Culicidae) From Sylvan Communities in Mexico"],"prefix":"10.3389","volume":"7","author":[{"given":"Luis M.","family":"Hern\u00e1ndez-Triana","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier A.","family":"Garza-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldo I.","family":"Ortega Morales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean W. J.","family":"Prosser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul D. N.","family":"Hebert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadya I.","family":"Nikolova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsa","family":"Barrero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erick de J.","family":"de Luna-Santillana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicente H.","family":"Gonz\u00e1lez-Alvarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram\u00f3n","family":"Mendez-L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahuel J.","family":"Chan-Chable","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony R.","family":"Fooks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario A.","family":"Rodr\u00edguez-P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,3,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1111\/1469-0691.12189","article-title":"Public health significance of invasive mosquitoes in Europe","volume":"19","author":"Schaffner","year":"2013","journal-title":"Clin Microbiol Infect."},{"key":"B2","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.ijid.2016.02.001","article-title":"Rapid spread of zika virus in the americas \u2013 implications for public health preparedness for mass gatherings at the 2016 Brazil olympic games","volume":"44","author":"Petersen","year":"2016","journal-title":"Internat J Infect Dis."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1673\/031.010.3201","article-title":"Host-feeding preference of the mosquito, Culex quinquefasciatus, in Yucatan State, Mexico","volume":"10","author":"Garcia-Rejon","year":"2010","journal-title":"J Insect Sci."},{"key":"B4","doi-asserted-by":"publisher","first-page":"e0134375","DOI":"10.1371\/journal.pone.0134375","article-title":"Unraveling host-vector-arbovirus interactions by two-gene high resolution melting mosquito bloodmeal analysis in a Kenyan Wildlife-livestock interface","volume":"10","author":"Omondi","year":"2015","journal-title":"PLoS ONE."},{"key":"B5","unstructured":"HarbachRE Mosquito Taxonomic Inventory"},{"key":"B6","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1111\/j.1755-0998.2012.03140.x","article-title":"Identifying the last supper: utility of the DNA barcode library for bloodmeal identification in ticks","volume":"12","author":"Gariepy","year":"2012","journal-title":"Mol Ecol Res."},{"key":"B7","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1016\/S1471-4922(02)02364-4","article-title":"Analysis of arthropod bloodmeals using molecular genetic markers","volume":"18","author":"Mukabana","year":"2002","journal-title":"Trends Parasit."},{"key":"B8","doi-asserted-by":"publisher","first-page":"336","DOI":"10.4269\/ajtmh.2005.73.336","article-title":"Identification of mammalian blood meals in mosquitoes by a multiplexed polymerase chain reaction targeting cytochrome B","volume":"73","author":"Kent","year":"2005","journal-title":"Am J Trop Med Hyg."},{"key":"B9","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1111\/j.1755-0998.2008.02469.x","article-title":"Molecular methods for arthropod bloodmeal identification and applications to ecological and vector-borne disease studies","volume":"9","author":"Kent","year":"2009","journal-title":"Mol Ecol Res."},{"key":"B10","doi-asserted-by":"publisher","first-page":"e0003628","DOI":"10.1371\/journal.pntd.0003628","article-title":"Xenosurveillance: a novel mosquito-based approach for examining the human-pathogen landscape","volume":"9","author":"Grubaugh","year":"2015","journal-title":"PLoS Neg Trop Dis."},{"key":"B11","doi-asserted-by":"publisher","first-page":"1377","DOI":"10.4269\/ajtmh.15-0440","article-title":"Feasibility of using mosquito blood meal for rapid and efficient human and animal virus surveillance and discovery","volume":"93","author":"Yang","year":"2015","journal-title":"Am J Trop Med Hyg."},{"key":"B12","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1111\/mve.12155","article-title":"Host preferences in host-seeking and blood-fed mosquitoes in Switzerland","volume":"30","author":"Sch\u00f6nenberger","year":"2016","journal-title":"Med Vet Entomol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"324","DOI":"10.4269\/ajtmh.17-0063","article-title":"The use of xenosurveillance to detect human, bacteria, parasites, and viruses in mosquitoes bloodmeals","volume":"97","author":"Fauver","year":"2017","journal-title":"Am J Trop Med Hyg."},{"key":"B14","doi-asserted-by":"publisher","first-page":"30006348","DOI":"10.1371\/journal.pntd.0006348","article-title":"Xenosurveillance reflects traditional samples techniques for the identification of human pathogens: a comparitive study in West Africa","volume":"12","author":"Fauver","year":"2018","journal-title":"PLoS ONE Negl Trop Dis."},{"key":"B15","volume-title":"Tracing Pathogen Transmission by Mosquitoes Under Global Change Perspective: on the Role of the Identification of Mosquitoes Bloodmeals","author":"Mart\u00ednez de la Fuente","year":"2018"},{"key":"B16","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1186\/1475-2875-12-109","article-title":"Effect of blood meal digestion and DNA extraction protocol on the success of blood meal source determination in the malaria vector Anopheles atroparvus","volume":"12","author":"Mart\u00ednez de la Fuente","year":"2013","journal-title":"Malaria J"},{"key":"B17","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1371\/journal.pone.0007092","article-title":"Disentangling vector-borne transmission networks: A universal DNA barcoding method to identify vertebrate hosts from arthropod bloodmeals","volume":"4","author":"Alcaide","year":"2009","journal-title":"PLoS ONE."},{"key":"B18","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1186\/1742-9994-7-3","article-title":"Blood feeding patterns of mosquitoes: random or structured?","volume":"7","author":"Chaves","year":"2010","journal-title":"Front in Zool."},{"key":"B19","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1186\/s13071-017-2098-4","article-title":"Blood-feeding patterns of native mosquitoes and insights into the potential role as pathogen vectors in the Thames estuary region of the United Kingdom","volume":"10","author":"Brugman","year":"2017","journal-title":"Parasite Vect"},{"key":"B20","doi-asserted-by":"publisher","first-page":"e39549","DOI":"10.1371\/journal.pone.0039549","article-title":"Feeding patterns of potential West Nile virus vectors in south-west Spain","volume":"7","author":"Mu\u00f1oz","year":"2012","journal-title":"PLoS ONE."},{"key":"B21","doi-asserted-by":"publisher","first-page":"67","DOI":"10.11646\/zootaxa.4250.1.5","article-title":"Molecular approaches for blood meal analysis and species identification of mosquitoes (Insecta: Diptera: Culicidae) in rural locations in southern England, United Kingdom","volume":"4250","author":"Hern\u00e1ndez-Triana","year":"2017","journal-title":"Zootaxa."},{"key":"B22","first-page":"1","article-title":"Keys to the adults, male hypopygia, fourth-instar larvae and pupae of the British mosquitoes (Culicidae) with notes on their ecology and medical importance","volume":"48","author":"Cranston","year":"1987","journal-title":"Freshw Biol Assoc Scient Pub."},{"key":"B23","doi-asserted-by":"publisher","first-page":"43","DOI":"10.11646\/zootaxa.3514.1.3","article-title":"The utility of DNA barcoding for species identification within the blackfly Subgenus Trichodagmia Enderlein (Diptera: Simuliidae: Simulium) and related taxa in the New World","volume":"3514","author":"Hern\u00e1ndez-Triana","year":"2012","journal-title":"Zootaxa."},{"key":"B24","doi-asserted-by":"publisher","first-page":"508","DOI":"10.1111\/1755-0998.12208","article-title":"Recovery of DNA barcodes from blackfly museum specimens (Diptera: Simuliidae) using primer sets that target a variety of sequence length","volume":"14","author":"Hern\u00e1ndez-Triana","year":"2014","journal-title":"Mol Ecol Res."},{"key":"B25","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1007\/s00436-015-4726-2","article-title":"DNA barcoding and molecular evolution of mosquito vectors of medical and veterinary importance","volume":"115","author":"Murugan","year":"2015","journal-title":"Parasit Res."},{"key":"B26","doi-asserted-by":"publisher","first-page":"e0212517","DOI":"10.1371\/journal.pone.0212517","article-title":"Molecular identification of blood meals in mosquitoes (Diptera, Culicidae) in urban and forested habitats in southern Brazil","volume":"14","author":"Santos","year":"2019","journal-title":"PLoS ONE."},{"key":"B27","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1098\/rspb.2002.2218","article-title":"Biological identifications through DNA barcodes","volume":"270","author":"Hebert","year":"2003","journal-title":"Proc R Soc Biolog Sci"},{"key":"B28","doi-asserted-by":"publisher","first-page":"S96","DOI":"10.1098\/rsbl.2003.0025","article-title":"Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species","volume":"270","author":"Hebert","year":"2003","journal-title":"Proc R Soc Biol Sci"},{"key":"B29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0097268","article-title":"Analyzing mosquito (Diptera : Culicidae) diversity in Pakistan by DNA barcoding","volume":"9","author":"Ashfaq","year":"2014","journal-title":"PLoS ONE."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13071-014-0569-4","article-title":"DNA barcoding: complementing morphological identification of mosquito species in Singapore","volume":"7","author":"Chan","year":"2014","journal-title":"Parasite Vect."},{"key":"B31","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1111\/j.1365-2915.2006.00653.x","article-title":"Identifying Canadian mosquito species through DNA barcodes","volume":"20","author":"Cywinska","year":"2006","journal-title":"Med Vet Entomol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"57","DOI":"10.3897\/zookeys.832.32257","article-title":"DNA barcoding of British mosquitoes (Diptera, Culicidae) to support species identification, discovery of cryptic genetic diversity and monitoring invasive species","volume":"832","author":"Hern\u00e1ndez-Triana","year":"2019","journal-title":"ZooKeys."},{"key":"B33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/jmedent\/41.5.01","article-title":"DNA barcodes can distinguish species of Indian mosquitoes (Diptera: Culicidae)","volume":"44","author":"Kumar","year":"2007","journal-title":"J Med Entomol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"e0176993","DOI":"10.1371\/journal.pone.0176993","article-title":"DNA reference libraries of French Guianese mosquitoes for barcoding and metabarcoding","volume":"12","author":"Talaga","year":"2017","journal-title":"PLoS ONE."},{"key":"B35","doi-asserted-by":"publisher","first-page":"e6596","DOI":"10.7717\/peerj.6596","article-title":"DNA metabarcoding allows non-invasive identification of arthropod prey provisioned to nestling Rufous hummingbirds (Selasphorus rufus)","volume":"7","author":"Moran","year":"2019","journal-title":"Peer J."},{"key":"B36","doi-asserted-by":"publisher","first-page":"998","DOI":"10.1111\/j.1471-8286.2006.01428.x","article-title":"An inexpensive, automation-friendly protocol for recovering high-quality DNA","volume":"6","author":"Ivanova","year":"2006","journal-title":"Mol Ecol Notes."},{"key":"B37","doi-asserted-by":"publisher","first-page":"263","DOI":"10.3958\/059.045.0127","article-title":"First record of Aedes (Ochlerotatus) fulvus in Tabasco and distribution notes of other Aedes in Mexico","volume":"45","author":"Rodr\u00edguez-Mart\u00ednez","year":"2020","journal-title":"South Entomol."},{"key":"B38","unstructured":"Direcci\u00f3n General de Epidemiolog\u00eda.2019"},{"key":"B39","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1089\/vbz.2008.0087","article-title":"West Nile virus activity in mosquitoes and domestic animals in Chiapas, Mexico","volume":"9","author":"Ulloa","year":"2009","journal-title":"Vector-Borne Zoon Dis"},{"key":"B40","doi-asserted-by":"publisher","first-page":"788","DOI":"10.1046\/j.1523-1739.1994.08030780.x","article-title":"Mammal diversity and conservation in the Selva Lacandon, Chiapas, Mexico","volume":"8","author":"Medellin","year":"1994","journal-title":"Cons Biol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1089\/153036603765627406","article-title":"Serologic survey of domestic animals for zoonotic arbovirus infections in the Lacand\u00f3n Forest region of Chiapas, Mexico","volume":"3","author":"Ulloa","year":"2003","journal-title":"Vector-Borne Zoon Dis"},{"key":"B42","doi-asserted-by":"publisher","first-page":"654","DOI":"10.2987\/8756-971X(2006)22[654:HPOCQI]2.0.CO;2","article-title":"Host-feeding preference of Culex quinquefasciatus in Monterrey, northeastern Mexico","volume":"22","author":"Elizondo-Quiroga","year":"2006","journal-title":"J Am Mosq Control Assoc."},{"key":"B43","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1093\/jmedent\/30.3.518","article-title":"Anopheles albimanus (Diptera: Culicidae) host selection patterns in three ecological areas of the coastal plains of Chiapas, southern Mexico","volume":"30","author":"Loyola","year":"1993","journal-title":"J Med Entomol."},{"key":"B44","doi-asserted-by":"publisher","first-page":"1488","DOI":"10.1111\/tmi.12587","article-title":"Mammalophilic feeding behaviour of Culex quinquefasciatus mosquitoes collected in the cities of Chetumal and Cancun, Yucat\u00e1n Peninsula, Mexico","volume":"20","author":"Janssen","year":"2019","journal-title":"Trop Med Internat Health."},{"key":"B45","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1016\/j.parint.2018.01.006","article-title":"Blood-meal preferences and avian malaria detection in mosquitoes (Diptera: Culicidae) captured at different land use types within a neotropical montane cloud forest matrix","volume":"67","author":"Abella-Medrano","year":"2018","journal-title":"Parasitol Internat."},{"key":"B46","doi-asserted-by":"publisher","first-page":"e0133602","DOI":"10.1371\/journal.pone.0133602","article-title":"Making mosquito taxonomy useful: a stable classification of tribe Aedini that balances utility with current knowledge of evolutionary relationships","volume":"10","author":"Wilkerson","year":"2015","journal-title":"PLoS ONE."},{"key":"B47","first-page":"611","article-title":"A catalog of the mosquitoes of the world (Diptera: Culicidae)","volume":"6","author":"Knight","year":"1977","journal-title":"Entomol Soc Am."},{"key":"B48","doi-asserted-by":"publisher","first-page":"218","DOI":"10.4319\/lom.2008.6.218","article-title":"Application of an inexpensive and high throughput genomic DNA extraction method for the molecular ecology of zooplanktonic diapausing eggs","volume":"6","author":"Montero-Pau","year":"2008","journal-title":"Limnol Oceaneog Meth."},{"key":"B49","first-page":"294","article-title":"DNA primers for amplification of mitochondrial cytochrome oxidase subunit I from diverse metazoan invertebrates","volume":"3","author":"Folmer","year":"1994","journal-title":"Mol Microb Biol Biotechnol."},{"key":"B50","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1111\/1755-0998.12474","article-title":"DNA barcodes from century-old type specimens using next generation sequencing","volume":"16","author":"Prosser","year":"2016","journal-title":"Mol Ecol Res."},{"key":"B51","doi-asserted-by":"publisher","first-page":"2725","DOI":"10.1093\/molbev\/mst197","article-title":"MEGA6: molecular evolutionary genetics analysis version 6.0.","volume":"30","author":"Tamura","year":"2013","journal-title":"Mol Biol Evol."},{"key":"B52","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1093\/sysbio\/syq010","article-title":"New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0","volume":"59","author":"Guindon","year":"2010","journal-title":"System Biol."},{"key":"B53","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1080\/10635150600755453","article-title":"Approximate likelihood-ratio test for branches:a fast, accurate, and powerful alternative","volume":"55","author":"Anisimova","year":"2006","journal-title":"System Biol."},{"key":"B54","doi-asserted-by":"publisher","first-page":"e66213","DOI":"10.1371\/journal.pone.0066213","article-title":"A DNA-based registry for all animal species: the barcode index number (BIN) system","volume":"8","author":"Ratnasingham","year":"2013","journal-title":"PLoS ONE."},{"key":"B55","doi-asserted-by":"publisher","first-page":"568835","DOI":"10.3389\/fevo.2020.568835","article-title":"Avian feeding preferences of Culex pipiens and Culiseta spp. Along and Urban-to-Wild Gradient in Northern Spain","volume":"8","author":"Gonz\u00e1lez","year":"2020","journal-title":"Front Ecol Evol."},{"key":"B56","doi-asserted-by":"publisher","first-page":"e0008867","DOI":"10.1371\/journal.pntd.0008867","article-title":"Vertebrate-Aedes aegypti and Culex quinquefasciatus (Diptera)-arbovirus transmission networks: non-human feeding revealed by meta-barcoding and next-generation sequencing","volume":"14","author":"Estrada-Franco","year":"2020","journal-title":"PLoS Negl Trop Dis."},{"key":"B57","doi-asserted-by":"publisher","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"CUTADAPT removes adapter sequences from high-throughput sequencing reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet J."},{"key":"B58","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1089\/vbz.2009.0210","article-title":"Assessment of a novel real-time Pan-Flavivirus RT-Polymerase Chain Reaction","volume":"10","author":"Johnson","year":"2010","journal-title":"Vector Borne Zoon Dis."},{"key":"B59","first-page":"298","article-title":"A survey and bibliography of the mosquito fauna of Mexico (Diptera: Culicidae)","volume":"12","author":"Darsie","year":"1996","journal-title":"J Am Mosq Cont Assoc."},{"key":"B60","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1016\/j.actatropica.2018.08.036","article-title":"Confirmation of occurrence of Anopheles (Anopheles) veruslanei Vargas in Quintana Roo, Mexico using morphology and DNA barcodes","volume":"188","author":"Chan-Chable","year":"2018","journal-title":"Acta Trop"},{"key":"B61","first-page":"7","article-title":"Especies cr\u00edpticas en Ochlerotatus taeniorhynchus mediante revelaci\u00f3n de c\u00f3digo de barras","volume":"11","author":"Chan-Chable","year":"2018","journal-title":"Salud Quintana Roo"},{"key":"B62","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1111\/jvec.12329","article-title":"The mosquitoes (Diptera: Culicidae) of Tabasco, Mexico","volume":"44","author":"Ortega-Morales","year":"2019","journal-title":"J Vect Ecol"},{"key":"B63","first-page":"616","volume-title":"The Mosquitoes of the Americas","author":"Dyar","year":"1928"},{"key":"B64","doi-asserted-by":"publisher","first-page":"237","DOI":"10.2987\/16-6580.1","article-title":"First record of Psorophora albipes in Quintana Roo, Mexico","volume":"32","author":"Chan-Chable","year":"2016","journal-title":"J Am Mosq Cont Assoc."},{"key":"B65","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1016\/j.ygeno.2018.03.016","article-title":"Description and phylogeny of the mitochondrial genome of Sabethes chloropterus, Sabethes glaucodaemon and Sabethes belisarioi (Diptera: Culicidae)","volume":"111","author":"de Oliveira Arag\u00e3o","year":"2019","journal-title":"Genome."},{"key":"B66","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1016\/j.virol.2007.06.016","article-title":"Direct broad-range detection of alphaviruses in mosquito extracts","volume":"368","author":"Eshoo","year":"2007","journal-title":"Virol."},{"key":"B67","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1016\/j.actatropica.2014.10.013","article-title":"Barcoding Turkish Culex mosquitoes to aciliate arbovirus vector incrimination studies reveals hidden diversity and new potential vectors","volume":"143","author":"Gunay","year":"2014","journal-title":"Acta Trop."},{"key":"B68","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1111\/1755-0998.12318","article-title":"Identification of Belgian mosquito species (Diptera: Culicidae) by DNA barcoding","volume":"15","author":"Versteirt","year":"2015","journal-title":"Mol Ecol Res."},{"key":"B69","doi-asserted-by":"publisher","first-page":"649","DOI":"10.4269\/ajtmh.1999.60.649","article-title":"Cytogenetic evidence for a species complex within Anopheles pseudopunctipennis Theobald (Diptera: Culicidae)","volume":"60","author":"Coetzee","year":"1991","journal-title":"Am J Trop Med Hyg."},{"key":"B70","first-page":"26","article-title":"Genetic evidence of a species complex in Anopheles pseudopunctipennis sensu lato","volume":"27","author":"Estrada-Franco","year":"1993","journal-title":"Bull Pan Am Heal Org."},{"key":"B71","doi-asserted-by":"publisher","first-page":"4692","DOI":"10.1002\/ece3.5073","article-title":"DNA barcodes and evidence of cryptic diversity of anthropophagous mosquitoes in Quintana Roo, Mexico","volume":"9","author":"Chan-Chable","year":"2019","journal-title":"Ecol Evol."},{"key":"B72","doi-asserted-by":"publisher","first-page":"105455","DOI":"10.1016\/j.actatropica.2020.105455","article-title":"Molecular and morphological evidence for sibling species within Anopheles (Anopheles) lindesayi Giles (Diptera: Culicidae) in Bhutan","volume":"207","author":"Namgay","year":"2020","journal-title":"Acta Trop."},{"key":"B73","first-page":"1","article-title":"Mosquito studies (Diptera, Culicidae) XX. The terrens group of Aedes (Finlaya)","volume":"5","author":"Schick","year":"1970","journal-title":"Cont Am Entomol Inst"},{"key":"B74","first-page":"13","article-title":"Mosquito studies (Diptera, Culicidae) XXIII. Additions and corrections to the Revision of Aedes terrens group","volume":"7","author":"Schick","year":"1970","journal-title":"Cont Am Entomol Inst"},{"key":"B75","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1590\/0074-0276130457","article-title":"COI barcode versus morphological identification of Culex (Culex) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil","volume":"108","author":"Laurito","year":"2013","journal-title":"Mem Instit Oswaldo Cruz."},{"key":"B76","first-page":"265","article-title":"A review of the systematics and a proposed scheme of internal classification of the new world subgenus Melanoconion of Culex (Diptera, Culicidae)","volume":"14","author":"Sirivanakarn","year":"1982","journal-title":"Mosq Syst."},{"key":"B77","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1016\/0016-3287(79)90128-9","article-title":"A reclassification of Sabethine genus Trichoprosopon","volume":"11","author":"Zavortink","year":"1979","journal-title":"Mosq Syst"},{"key":"B78","first-page":"1","article-title":"Especies y distribuci\u00f3n de mosquitos mexicanos no Anofelinos (Insecta Diptera)","volume":"16","author":"Vargas","year":"1956","journal-title":"Rev Inst Salub Enf Trop."},{"key":"B79","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1590\/S0074-02761931000100004","article-title":"Nota sobre sabethineos do grupo Joblotia (Diptera: Culicidae)","volume":"25","author":"Costa Lima","year":"1931","journal-title":"Mem Inst Oswaldo Cruz."},{"key":"B80","doi-asserted-by":"crossref","first-page":"473","DOI":"10.11606\/issn.2176-7793.19423473","article-title":"Os Sabetineos da America (Diptera, Culicidae)","volume":"3","author":"Lane","year":"1942","journal-title":"Arq Zool."},{"key":"B81","first-page":"151","article-title":"The mosquitoes of Guatemala, their identification, distribution and bionomics","volume":"15","author":"Clark-Gil","year":"1983","journal-title":"Mosq Systemat."},{"key":"B82","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1590\/0074-0276130440","article-title":"Mosquitoes of eastern Amazonian Ecuador: biodiversity, bionomics and barcodes","volume":"108","author":"Linton","year":"2013","journal-title":"Mem Inst Oswaldo Cruz."},{"key":"B83","first-page":"133","article-title":"Diagnoses of new species of mosquitoes","volume":"19","author":"Dyar","year":"1906","journal-title":"Proc Biol Soc."},{"key":"B84","first-page":"40","volume-title":"The Mosquitoes or Culicidae of Jamaica","author":"Theobald","year":"1905"},{"key":"B85","first-page":"553","volume-title":"Neotropical Culicidae Vol. II","author":"Lane","year":"1953"},{"key":"B86","first-page":"495","author":"Carpenter","year":"1955","journal-title":"Mosquitoes of North America (North of Mexico)"},{"key":"B87","doi-asserted-by":"publisher","first-page":"384","DOI":"10.5479\/si.00810282.16","article-title":"Breding-Archbold-Smithsonian biological survey of Dominica: The mosquitoes of Dominica (Diptera: Culicidae)","volume":"70","author":"Stone","year":"1969","journal-title":"Proc Entomol Soc."},{"key":"B88","first-page":"1","article-title":"The Culicidae of Jamaica (Mosquito Studies. XXI)","volume":"6","author":"Belkin","year":"1970","journal-title":"Contrib Am Entomol Inst"},{"key":"B89","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1093\/jme\/tjy237","article-title":"Bloodmeal host selection of Culex quinquefasciatus (Diptera: Culicidae) in Las Vegas, Nevada, United States","volume":"56","author":"Hannon","year":"2019","journal-title":"J Med Entomol."},{"key":"B90","doi-asserted-by":"publisher","first-page":"73","DOI":"10.4269\/ajtmh.2007.77.73","article-title":"Host feeding pattern of Culex quinquefasciatus (Diptera: Culicidae) and its role in transmission of West Nile virus in Harris County, Texas","volume":"77","author":"Molaei","year":"2007","journal-title":"Am J Trop Med Hyg."},{"key":"B91","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1016\/j.actatropica.2018.07.003","article-title":"The mosquitoes (Diptera: Culicidae) of Hidalgo state, Mexico","volume":"189","author":"Ortega-Morales","year":"2018","journal-title":"Acta Trop"},{"key":"B92","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1186\/s13071-015-0705-9","article-title":"Enhanced West Nile virus surveillance in the North Kent marshes, UK","volume":"8","author":"Vaux","year":"2015","journal-title":"Parasit Vect."},{"key":"B93","doi-asserted-by":"publisher","first-page":"4869","DOI":"10.3390\/ijerph10104869","article-title":"European surveillance for West Nile virus in mosquito populations","volume":"10","author":"Engler","year":"2013","journal-title":"Int J Environ Res Public Health."},{"key":"B94","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1186\/s41182-018-0117-6","article-title":"Survey of mosquito-borne flaviviruses in the cuitzmala river basin: do they circulate in rodents and bats?","volume":"46","author":"Sotomayor-Bonilla","year":"2018","journal-title":"Trop Med Health."},{"key":"B95","doi-asserted-by":"publisher","first-page":"726","DOI":"10.1007\/s10393-019-01442-2","article-title":"Insights into the host-specificity of mosquito-borne flaviviruses infecting wild mammals","volume":"16","author":"Sotomaryor-Bonilla","year":"2019","journal-title":"EcoHealth."},{"key":"B96","doi-asserted-by":"publisher","first-page":"129","DOI":"10.4269\/ajtmh.13-0524","article-title":"Dengue virus in bats from southeastern Mexico","volume":"91","author":"Sotomayor-Bonilla","year":"2014","journal-title":"Am J Trop Med Hyg."}],"container-title":["Frontiers in Veterinary Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2020.564791\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,25]],"date-time":"2024-08-25T19:15:29Z","timestamp":1724613329000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2020.564791\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,10]]},"references-count":96,"alternative-id":["10.3389\/fvets.2020.564791"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2020.564791","relation":{},"ISSN":["2297-1769"],"issn-type":[{"value":"2297-1769","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,10]]},"article-number":"564791"}}