{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:14:57Z","timestamp":1771654497146,"version":"3.50.1"},"reference-count":20,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T00:00:00Z","timestamp":1610582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>Leptospirosis is a global zoonotic disease caused by pathogenic <jats:italic>Leptospira<\/jats:italic>, and those infected animals will show a variety of clinical symptoms and even death. The discovery of endemic strains is crucial to produce effective vaccines. In this study, we report that a strain of <jats:italic>Leptospira<\/jats:italic>, isolated from a dog, is pathogenic. Using MLST analysis, the serovar of isolated <jats:italic>Leptospira<\/jats:italic> was identified and found it belongs to <jats:italic>Leptospira interrogation<\/jats:italic> Serovar Australis. Then, the virulence of this strain was researched by using hamsters. After infection, all the hamsters died within 4\u20135 days. Typical pathological changes were found in the liver, kidney, and lung of hamsters. These results all indicated that the isolated <jats:italic>Leptospira<\/jats:italic> was pathogenic. Thus, this study facilitates to identifying local <jats:italic>Leptospira<\/jats:italic> strains and develop a more targeted canine <jats:italic>Leptospira<\/jats:italic> vaccine.<\/jats:p>","DOI":"10.3389\/fvets.2020.607115","type":"journal-article","created":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T19:33:01Z","timestamp":1610652781000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Preliminary Characterization of Dog Derived Pathogenic Strains of Leptospira interrogans Serovar Australis in Nanchang of Jiangxi Province, China"],"prefix":"10.3389","volume":"7","author":[{"given":"Ning","family":"Song","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenlong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Ding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dianjun","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zonghao","family":"Dai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongguo","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,1,14]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/j.vetmic.2009.03.012","article-title":"Leptospira and Leptospirosis","volume":"140","author":"Adler","year":"2010","journal-title":"Vet Microbiol"},{"key":"B2","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1590\/S0036-46652003000500015","article-title":"Human Leptospirosis: guidance for diagnosis, surveillance and control","volume":"45","author":"Romero","year":"2003","journal-title":"Rev Inst Med Trop S\u00e3o Paulo."},{"key":"B3","doi-asserted-by":"publisher","first-page":"883","DOI":"10.12659\/AJCR.900477","article-title":"Leptospirosis-associated severe pulmonary hemorrhagic syndrome with lower back pain as an initial symptom","volume":"17","author":"S\u00f8ndergaard","year":"2016","journal-title":"Am J Case Rep."},{"key":"B4","doi-asserted-by":"publisher","first-page":"e7093","DOI":"10.1371\/journal.pone.0007093","article-title":"Development and validation of a real-time PCR for detection of pathogenic Leptospira species in clinical materials","volume":"4","author":"Ahmed","year":"2009","journal-title":"PLoS ONE."},{"key":"B5","doi-asserted-by":"publisher","first-page":"432","DOI":"10.1097\/QCO.0000000000000097","article-title":"Leptospira and leptospirosis in China","volume":"27","author":"Weilin","year":"2014","journal-title":"Curr Opin Infect Dis."},{"key":"B6","doi-asserted-by":"publisher","first-page":"1224","DOI":"10.2460\/javma.2003.222.1224","article-title":"Clinical application of a polymerase chain reaction assay for diagnosis of Leptospirosis in dogs","volume":"222","author":"Harkin","year":"2003","journal-title":"J Am Vet Med Assoc."},{"key":"B7","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/978-3-662-45059-8_5","article-title":"Leptospirosis in humans","volume":"387","author":"Haake","year":"2015","journal-title":"Curr Top Microbiol Immunol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1007\/978-3-662-45059-8_6","article-title":"Animal leptospirosis","volume":"387","author":"Ellis","year":"2015","journal-title":"Curr Top Microbiol Immunol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"e0005191","DOI":"10.1371\/journal.pntd.0005191","article-title":"Efficacy of the rabbit polyclonal anti-Leptospira antibody against homotype or heterotype Leptospira infection in hamster","volume":"10","author":"Jin","year":"2016","journal-title":"PLoS Negl Trop Dis."},{"key":"B10","doi-asserted-by":"publisher","first-page":"e1954","DOI":"10.1371\/journal.pntd.0001954","article-title":"A Single Multilocus Sequence Typing (MLST) scheme for seven pathogenic Leptospira species","volume":"7","author":"Boonsilp","year":"2013","journal-title":"PLoS Negl. 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