{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T15:15:07Z","timestamp":1784128507425,"version":"3.55.0"},"reference-count":16,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T00:00:00Z","timestamp":1614729600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>The microscopic agglutination test (MAT) used for the serological diagnosis of leptospirosis, as a robust and inexpensive method, is still the reality in many laboratories worldwide. Both the performance and the interpretation of the MAT vary from region to region, making standardization difficult. The prediction of the probable infecting serogroup using this test is indispensable for elucidating the epidemiology of the disease; however, in veterinary medicine, many studies consider any reaction detected with a titer of 100, which may ultimately overestimate some serogroups. Thus, the aim of this study was to evaluate the usefulness of the ranking technique for predicting the probable infecting serogroup identified by the MAT, eliminating cross reactions with other serogroups. <jats:italic>Leptospira<\/jats:italic> strains (12 samples) were inoculated in hamsters, and after 30 days, serology was performed by the MAT for these animals to confirm the infecting serogroup. Using the ranking technique, the probable infectious serogroup found with the MAT was the same as that in which the strains of inoculated leptospires belonged; additionally, the technique can be applied in epidemiological studies involving herds.<\/jats:p>","DOI":"10.3389\/fvets.2021.654034","type":"journal-article","created":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T04:27:38Z","timestamp":1614745658000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Usefulness of the Ranking Technique in the Microscopic Agglutination Test (MAT) to Predict the Most Likely Infecting Serogroup of Leptospira"],"prefix":"10.3389","volume":"8","author":[{"given":"Israel Barbosa","family":"Guedes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisele Oliveira de","family":"Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Fernandes de Paula","family":"Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus Burilli","family":"Cavalini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ant\u00f4nio Francisco","family":"de Souza Filho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Bryan","family":"Heinemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,3,3]]},"reference":[{"key":"B1","first-page":"272","volume-title":"Leptospira and Leptospirosis","author":"Faine","year":"1999"},{"key":"B2","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1128\/CMR.14.2.296-326.2001","article-title":"Leptospirosis","volume":"13","author":"Levett","year":"2001","journal-title":"Clin Microbiol Rev."},{"key":"B3","doi-asserted-by":"publisher","first-page":"e0003607","DOI":"10.1371\/journal.pntd.0003607","article-title":"Predominant leptospiral serogroups circulating among humans, livestock and wildlife in Katavi-Rukwa ecosystem, Tanzania","volume":"9","author":"Assenga","year":"2015","journal-title":"PLoS Negl Trop Dis."},{"key":"B4","volume-title":"Human Leptospirosis: Guidance for Diagnosis, Surveillance and Control","year":"2003"},{"key":"B5","first-page":"7","article-title":"Leptospirose bovina. N\u00edveis de ocorr\u00eancia e sorotipos predominantes em rebanhos dos Estados de Minas Gerais, S\u00e3o Paulo, Rio de Janeiro, Paran\u00e1, Rio Grande do Sul e Mato Grosso do Sul, no per\u00edodo de janeiro a abril de 1996","volume":"64","author":"Vasconcellos","year":"1997","journal-title":"Arq Inst Biol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1590\/S0103-84782002000400011","article-title":"Most frequent serovar of Leptospires in serological tests of buffaloes, sheeps, goats, horses, swines and dogs from several Brazilian states","volume":"32","author":"Favero","year":"2002","journal-title":"Cienc Rural."},{"key":"B7","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1590\/S0100-736X2012000700003","article-title":"Use of Leptospira spp. strains isolated in Brazil in the microscopic agglutination test applied to diagnosis of leptospirosis in cattle herds in eight Brazilian states","volume":"32","author":"Sarmento","year":"2012","journal-title":"Pesq Vet Bras."},{"key":"B8","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1002\/vms3.271","article-title":"Prevalence of Leptospira serogroups in buffaloes from the Brazilian Amazon","volume":"6","author":"Guedes","year":"2020","journal-title":"Vet Med Sci."},{"key":"B9","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1086\/346208","article-title":"Usefulness of serologic analysis as a predictor of the infecting serovar in patients with severe leptospirosis","volume":"36","author":"Levett","year":"2003","journal-title":"Clin Infect Dis."},{"key":"B10","doi-asserted-by":"publisher","first-page":"e101579","DOI":"10.1016\/j.cimid.2020.101579","article-title":"Leptospira strains isolated from cattle in the Amazon region, Brazil, evidence of a variety of species and serogroups with a high frequency of the Sejroe serogroup","volume":"74","author":"Guedes","year":"2021","journal-title":"Comp Immunol Microbiol Infect Dis."},{"key":"B11","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1016\/S0580-9517(08)70493-8","article-title":"Chapter VIII serological typing methods of leptospires","volume":"11","author":"Dikken","year":"1978","journal-title":"Methods Microbiol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/978-3-662-45059-8_5","article-title":"Leptospirosis in humans","volume":"387","author":"Haake","year":"2015","journal-title":"Curr Top Mirobiol Immunol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1177\/0300985811409252","article-title":"Development of chronic and acute golden Syrian hamster infection models with Leptospira borgpetersenii serovar Hardjo","volume":"49","author":"Zuerner","year":"2012","journal-title":"Vet Pathol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.theriogenology.2019.09.011","article-title":"Genital bovine leptospirosis: a new look for an old disease","volume":"141","author":"Loureiro","year":"2020","journal-title":"Theriogenology."},{"key":"B15","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/j.actatropica.2017.04.032","article-title":"Plurality of Leptospira strains on slaughtered animals suggest a broader concept of adaptability of leptospires to cattle","volume":"172","author":"Pinto","year":"2017","journal-title":"Acta Trop."},{"key":"B16","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/j.micpath.2017.05.001","article-title":"Seronegativity of bovines face to their own recovered leptospiral isolates","volume":"108","author":"Libonati","year":"2017","journal-title":"Microb Pathog."}],"container-title":["Frontiers in Veterinary Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2021.654034\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T04:27:44Z","timestamp":1614745664000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2021.654034\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,3]]},"references-count":16,"alternative-id":["10.3389\/fvets.2021.654034"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2021.654034","relation":{},"ISSN":["2297-1769"],"issn-type":[{"value":"2297-1769","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,3]]},"article-number":"654034"}}