{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T07:15:43Z","timestamp":1766733343023,"version":"3.41.2"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T00:00:00Z","timestamp":1638144000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>Proliferative enteropathy (PE) is an infectious enteric disease caused by <jats:italic>Lawsonia intracellularis<\/jats:italic> (<jats:italic>L. intracellularis<\/jats:italic>) and is endemic in pig herds worldwide. However, a <jats:italic>L. intracellularis<\/jats:italic>-specific monoclonal antibody plays an important role in the evaluation of <jats:italic>L. intracellularis<\/jats:italic> infection <jats:italic>in vitro<\/jats:italic>. Therefore, the objective of this study was to produce and identify the characteristics of a new monoclonal antibody against the outer membrane protein (Omp2) of <jats:italic>L. intracellularis<\/jats:italic> and apply it in an indirect immunofluorescence assay (IFA) and immunocytochemistry (IHC). The results indicated that three highly specific monoclonal antibodies against the Omp2 protein (4D9, 3G2, and 7G5) of <jats:italic>L. intracellularis<\/jats:italic> were obtained by using purified Omp2 as an immunogen, the titers of ascitic fluids of 4D9, 3G2, and 7G5 cells were 1:2,048,000, 1:512,000, and 1:256,000, respectively. IFA analysis showed that the 4D9, 3G2, and 7G5 have no cross-reactivity with other enteric bacteria commonly found in the ilea of pigs or closely related to <jats:italic>L. intracellularis<\/jats:italic>, such as <jats:italic>Desulfovibrio, Bilophila wadsworthia<\/jats:italic> (<jats:italic>B. wadsworthia<\/jats:italic>), <jats:italic>Salmonella choleraesuis<\/jats:italic> (<jats:italic>S. choleraesuis<\/jats:italic>), <jats:italic>Salmonella typhimurium<\/jats:italic> (<jats:italic>S. typhimurium<\/jats:italic>), <jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>E. coli<\/jats:italic>), and <jats:italic>Brachyspira hyodysenteriae<\/jats:italic> (<jats:italic>B. hyodysenteriae<\/jats:italic>). IFA and IHC results indicated that the monoclonal antibodies can be successfully used as primary antibodies to detect <jats:italic>L. intracellularis<\/jats:italic> in infected cells and in the crypt of the ileum from infected tissues of PE. Our findings suggested that the new monoclonal antibody specific against <jats:italic>L. intracellularis<\/jats:italic> will be useful for the evaluation of <jats:italic>L. intracellularis<\/jats:italic> infection <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>.<\/jats:p>","DOI":"10.3389\/fvets.2021.753610","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T13:41:14Z","timestamp":1638366074000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Preparation and Characterization of a New Monoclonal Antibody Specific Against Lawsonia intracellularis and Its Application in Indirect Immunofluorescence and Immunocytochemistry 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