{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T09:38:07Z","timestamp":1763545087597,"version":"3.41.2"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T00:00:00Z","timestamp":1638144000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>In the present study, we screened 502 natural product compounds against the <jats:italic>in vitro<\/jats:italic> growth of <jats:italic>Babesia<\/jats:italic> (<jats:italic>B<\/jats:italic>.) <jats:italic>bovis<\/jats:italic>. Then, the novel and potent identified compounds were further evaluated for their <jats:italic>in vitro<\/jats:italic> efficacies using viability and cytotoxicity assays. The <jats:italic>in vivo<\/jats:italic> inhibitory effects of the selected compounds were evaluated using <jats:italic>B. microti<\/jats:italic> \u201crodent strain\u201d in mice model. Three potent compounds, namely, Rottlerin (RL), Narasin (NR), Lasalocid acid (LA), exhibited the lowest IC<jats:sub>50<\/jats:sub> (half-maximal inhibitory concentration) as follows: 5.45 \u00b1 1.20 \u03bcM for RL, 1.86 \u00b1 0.66 \u03bcM for NR, and 3.56 \u00b1 1.41 \u03bcM for LA. The viability result revealed the ability of RL and LA to prevent the regrowth of treated parasite at 4 \u00d7 IC<jats:sub>50<\/jats:sub> and 2 \u00d7 IC<jats:sub>50<\/jats:sub>, respectively, while 4 \u00d7 IC<jats:sub>50<\/jats:sub> of NR was sufficient to stop the regrowth of parasite. The hematology parameters of <jats:italic>B. microti in vivo<\/jats:italic> were different in the NR-treated groups as compared to the infected\/untreated group. Interestingly, intraperitoneal administration of NR exhibiting inhibition in the growth of <jats:italic>B. microti<\/jats:italic> in mice was similar to that observed after administration of the commonly used antibabesial drug, diminazene aceturate (DA) (76.57% for DA, 74.73% for NR). Our findings indicate the richness of natural product compounds by novel potent antibabesial candidates, and the identified potent compounds, especially NR, might be used for the treatment of animal babesiosis.<\/jats:p>","DOI":"10.3389\/fvets.2021.762107","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T13:10:07Z","timestamp":1638364207000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Discovering the Potent Inhibitors Against Babesia bovis in vitro and Babesia microti in vivo by Repurposing the Natural Product Compounds"],"prefix":"10.3389","volume":"8","author":[{"given":"Yongchang","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed Abdo","family":"Rizk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eloiza 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