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Sci."],"abstract":"<jats:p><jats:italic>Ancylostoma caninum<\/jats:italic> is a zoonotic nematode which is able to affect animals and humans. Diagnosis in the definitive host and environmental detection are key to prevent its dissemination and achieve control. Herein, a new coprological LAMP method for the detection of <jats:italic>A. caninum<\/jats:italic> (Copro-LAMPAc) DNA was developed. DNA extraction was performed using a low-cost method and a fragment of the <jats:italic>cox<\/jats:italic>-1 gene was used for primer design. The analytical sensitivity, evaluated with serial dilutions of genomic DNA from <jats:italic>A. caninum<\/jats:italic> adult worms, was 100 fg. A specificity of 100% was obtained using genomic DNA from the host and other pathogens. The Copro-LAMPAc was evaluated using environmental canine fecal samples. When compared with gold standard optical microscopy in epidemiological studies, it proved to be more sensitive. 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