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Vet. Sci."],"abstract":"<jats:p><jats:italic>Sarcocystis<\/jats:italic> spp., <jats:italic>Neospora caninum<\/jats:italic> and <jats:italic>Toxoplasma gondii<\/jats:italic> are globally ubiquitous pathogens, and domestic sheep are considered to be one of the intermediate hosts. 83 myocardial samples of sheep were collected from 12 retail stores in Beijing, China. <jats:italic>Sarcocystis<\/jats:italic> spp., <jats:italic>N. caninum<\/jats:italic> and <jats:italic>T. gondii<\/jats:italic> were identified by PCR amplification of the 18S rRNA gene, Nc-5 gene and 529bp DNA fragment with a prevalence of 86.7% (95% CI: 77.5\u201393.2) and 43.4% (95% CI: 32.5\u201354.7) for <jats:italic>Sarcocystis<\/jats:italic> spp. and <jats:italic>N. caninum<\/jats:italic> infections, respectively, and no <jats:italic>T. gondii<\/jats:italic> was detected. The co-infection prevalence of <jats:italic>Sarcocystis<\/jats:italic> and <jats:italic>N. caninum<\/jats:italic> was 38.6% (95% CI: 28.1\u201349.9). Two <jats:italic>Sarcocystis<\/jats:italic> species were subtyped by analyzing 18SrRNA sequences and were identified as <jats:italic>Sarcocystis tenella<\/jats:italic> and <jats:italic>Sarcocystis arieticanis<\/jats:italic>. The prevalence of <jats:italic>S. tenella<\/jats:italic> and <jats:italic>S. arieticanis<\/jats:italic> infections was 84.3% (95% CI: 74.7\u201391.4) and 56.6% (95% CI: 45.3\u201367.5), respectively. This study shows that sheep have a high risk of infection with <jats:italic>Sarcocystis<\/jats:italic> and <jats:italic>N. caninum<\/jats:italic>, suggests that effective prevention measures are needed to avoid the spread of these parasites in sheep. Toxoplasmosis in sheep poses a threat to human and animal health and requires monitoring and preventing continuously.<\/jats:p>","DOI":"10.3389\/fvets.2022.1018788","type":"journal-article","created":{"date-parts":[[2022,9,30]],"date-time":"2022-09-30T09:59:48Z","timestamp":1664531988000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Molecular detection and identification of three intracellular parasites of retail mutton products in Beijing, 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