{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T01:13:42Z","timestamp":1789175622587,"version":"build-2803163510"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T00:00:00Z","timestamp":1667865600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013223","name":"Chongqing Research Program of Basic Research and Frontier Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013223","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>This study investigated the effect of Danggui Buxue decoction (DBD) on the immunity of an O-type foot-and-mouth disease (FMD) vaccine and intestinal mucosal immunity. SPF KM mice were continuously and orally administered DBD for 5 d and then inoculated with an O-type FMD vaccine. The contents of a specific IgG antibody and its isotypes IgG1, IgG2a, IgG2b, and IgG3 in serum and SIgA in duodenal mucosa were determined by ELISA at 1 and 3 W after the 2<jats:sup>nd<\/jats:sup>immunization. qRT-PCR was used to detect mRNA expression levels of IL-4, IL-10, IFN-\u03b3, and IL-33 in the spleen, and mRNA expression levels of J-chain, pIgR, BAFF, APRIL, IL-10, IFN-\u03b3 and IL-33 in the duodenum. The results showed that compared with the control group, oral administration of DBD significantly increased levels of the anti-FMD virus (FMDV)-specific antibodies IgG, IgG1, and IgG2a in the serum of O-type FMD vaccine-immunized mice 1 W after the 2<jats:sup>nd<\/jats:sup>immunization (<jats:italic>P<\/jats:italic>&amp;lt;<jats:italic>0.05<\/jats:italic>), upregulated mRNA expression levels of spleen lymphocyte cytokines IL-4 and IL-33 (<jats:italic>P<\/jats:italic>&amp;lt;<jats:italic>0.05<\/jats:italic>), promoted the secretion of SIgA in duodenal mucosa (<jats:italic>P<\/jats:italic>&amp;lt;<jats:italic>0.05<\/jats:italic>). The mRNA expression levels of J-chain, pIgR, BAFF, APRIL, IL-10, and IL-33 in duodenal tissues were upregulated (<jats:italic>P<\/jats:italic>&amp;lt;<jats:italic>0.05<\/jats:italic>). This study indicates that DBD has a good promotion effect on the O-type FMD vaccine and the potential to be an oral immune booster.<\/jats:p>","DOI":"10.3389\/fvets.2022.1045152","type":"journal-article","created":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T07:48:04Z","timestamp":1667893684000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Enhancement of intestinal mucosal immunity and immune response to the foot-and-mouth disease vaccine by oral administration of danggui buxue 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