{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T16:44:38Z","timestamp":1784306678522,"version":"3.55.0"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,12,20]],"date-time":"2022-12-20T00:00:00Z","timestamp":1671494400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>Intrauterine exosomes have been identified to be involved in the embryo development and implantation. The aim of this study was to explore the role of miRNAs in intrauterine exosomes in bovine pregnancy. Intrauterine exosomes were collected from uterine flushing fluids of three donor and three recipient Xianan cows 7 days after fertilization. Intrauterine exosomes miRNAs were extracted and the exosomal miRNAs expression levels were analyzed. Sixty miRNAs differed significantly in their amounts between donors and recipients (<jats:italic>p-value<\/jats:italic> &amp;lt; 0.05, |log2(FoldChange)| &amp;gt; 1). Twenty-two miRNAs were upregulated and 38 downregulated in the group of donor cows. The bta-miR-184 was the most significant (<jats:italic>P<\/jats:italic><jats:sub>Benjamini-Hochberg<\/jats:sub> &amp;lt; 0.001). A total of 9,775 target genes were predicted using the 60 miRNAs. GO and KEGG analysis showed that the target genes were enriched in several biological processes or pathways associated with embryo implantation and endometrial development, such as cell adhesion, cell junction, focal adhesion, and Rap1 signaling pathway. Our findings suggest that, in cattle early pregnancy stage, these differently expressed miRNAs in intrauterine exosomes involved in embryo implantation and endometrial development, which may exert a significant effect and influence the uterine microenvironment for embryo implantation. These results could provide reference for screening and exploring the intrauterine exosomal miRNA affecting embryo implantation.<\/jats:p>","DOI":"10.3389\/fvets.2022.1078394","type":"journal-article","created":{"date-parts":[[2022,12,20]],"date-time":"2022-12-20T04:44:32Z","timestamp":1671511472000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["miRNA profiling in intrauterine exosomes of pregnant cattle on day 7"],"prefix":"10.3389","volume":"9","author":[{"given":"Yaying","family":"Zhai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoting","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuxia","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuying","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajie","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinglei","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danny","family":"Arends","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gudrun A.","family":"Brockmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eryao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijie","family":"Lyu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,12,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1016\/j.theriogenology.2016.04.037","article-title":"Pivotal periods for pregnancy loss during the first trimester of gestation in lactating dairy cows","volume":"86","author":"Wiltbank","year":"2016","journal-title":"Theriogenology."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1916","DOI":"10.3390\/nu7031916","article-title":"Folate deficiency decreases apoptosis of endometrium decidual cells in pregnant mice via the mitochondrial pathway","volume":"7","author":"Liao XG Li","year":"2015","journal-title":"Nutrients."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1140","DOI":"10.1093\/biolre\/ioab149","article-title":"Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers","volume":"105","author":"Silva","year":"2021","journal-title":"Biol Reprod."},{"key":"B4","doi-asserted-by":"publisher","first-page":"285","DOI":"10.3390\/biology10040285","article-title":"Mesenchymal stem cell-derived exosomes and their therapeutic potential for osteoarthritis","volume":"10","author":"Kim","year":"2021","journal-title":"Biology."},{"key":"B5","doi-asserted-by":"publisher","first-page":"15","DOI":"10.3410\/B3-15","article-title":"Exosomes: secreted vesicles and intercellular communications","volume":"3","author":"Thery","year":"2011","journal-title":"Biol Rep."},{"key":"B6","doi-asserted-by":"publisher","first-page":"105247","DOI":"10.1126\/science.aau6977","article-title":"The biology, function, and biomedical applications of exosomes","volume":"367","author":"Kalluri","year":"2020","journal-title":"Science."},{"key":"B7","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1016\/j.theriogenology.2020.01.083","article-title":"Extracellular vesicles: novel regulators of conceptus-uterine interactions?","volume":"150","author":"O'Neil","year":"2020","journal-title":"Theriogenology."},{"key":"B8","doi-asserted-by":"publisher","first-page":"1510","DOI":"10.3390\/biom10111510","article-title":"The biological function of extracellular vesicles during fertilization, early embryo-maternal crosstalk and their involvement in reproduction: review and overview","volume":"10","author":"Capra","year":"2020","journal-title":"Biomolecules."},{"key":"B9","doi-asserted-by":"publisher","first-page":"e90913","DOI":"10.1371\/journal.pone.0090913","article-title":"Extracellular vesicles in luminal fluid of the ovine uterus","volume":"9","author":"Burns","year":"2014","journal-title":"PLoS ONE."},{"key":"B10","doi-asserted-by":"publisher","first-page":"1370","DOI":"10.1016\/j.bbrc.2017.11.176","article-title":"Intrauterine exosomes are required for bovine conceptus implantation","volume":"495","author":"Kusama","year":"2018","journal-title":"Biochem Biophys Res Commun."},{"key":"B11","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1530\/REP-14-0538","article-title":"Exosomes, endogenous retroviruses and toll-like receptors: pregnancy recognition in ewes","volume":"149","author":"Ruiz-Gonzalez","year":"2015","journal-title":"Reproduction."},{"key":"B12","doi-asserted-by":"publisher","first-page":"e0158278","DOI":"10.1371\/journal.pone.0158278","article-title":"Induction of ifnt-stimulated genes by conceptus-derived exosomes during the attachment period","volume":"11","author":"Nakamura","year":"2016","journal-title":"PLoS ONE."},{"key":"B13","doi-asserted-by":"publisher","first-page":"4264","DOI":"10.3390\/ijms21124264","article-title":"Exosomes as messengers between mother and fetus in pregnancy","volume":"21","author":"Czernek","year":"2020","journal-title":"Int J Mol Sci."},{"key":"B14","doi-asserted-by":"publisher","first-page":"3210","DOI":"10.1242\/dev.124289","article-title":"Hsa-Mir-30d, secreted by the human endometrium, is taken up by the pre-implantation embryo and might modify its transcriptome","volume":"142","author":"Vilella","year":"2015","journal-title":"Development."},{"key":"B15","doi-asserted-by":"publisher","first-page":"2308","DOI":"10.3390\/cells10092308","article-title":"Differential expression pattern of goat uterine fluids extracellular vesicles mirnas during peri-implantation","volume":"10","author":"Xie","year":"2021","journal-title":"Cells."},{"key":"B16","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1093\/nar\/gkg0006","article-title":"Rfam: an RNA family database","volume":"31","author":"Griffiths-Jones","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"B17","doi-asserted-by":"publisher","first-page":"D154","DOI":"10.1093\/nar\/gkm952","article-title":"Mirbase: tools for microrna genomics","volume":"36","author":"Griffiths-Jones","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"B18","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1093\/nar\/gkr688","article-title":"Mirdeep2 accurately identifies known and hundreds of novel microrna genes in seven animal clades","volume":"40","author":"Friedlander","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"B19","first-page":"1","article-title":"Analysing RNA-Seq data with the Deseq package","volume":"43","author":"Anders","year":"2010","journal-title":"Mol Biol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"R1","DOI":"10.1186\/gb-2003-5-1-r1","article-title":"Microrna targets in Drosophila","volume":"5","author":"Enright","year":"2003","journal-title":"Genome Biol"},{"key":"B21","doi-asserted-by":"publisher","first-page":"1514","DOI":"10.1016\/j.ygeno.2021.03.028","article-title":"Comparative profile of exosomal micrornas in postmenopausal women with various bone mineral densities by small RNA sequencing","volume":"113","author":"Kong","year":"2021","journal-title":"Genomics."},{"key":"B22","doi-asserted-by":"publisher","first-page":"1188","DOI":"10.1210\/en.2017-03082","article-title":"Embryo implantation: war in times of love","volume":"159","author":"Ashary","year":"2018","journal-title":"Endocrinology."},{"key":"B23","doi-asserted-by":"publisher","first-page":"155","DOI":"10.3390\/cells9010155","article-title":"Rap1 is involved in angiopoietin-1-induced cell-cell junction stabilization and endothelial cell sprouting","volume":"9","author":"Gaonac'h-Lovejoy","year":"2020","journal-title":"Cells"},{"key":"B24","doi-asserted-by":"publisher","first-page":"5075","DOI":"10.1007\/s11033-021-06502-x","article-title":"Endometrial gene expression profiling of recurrent implantation failure after in vitro fertilization","volume":"48","author":"Albayrak","year":"2021","journal-title":"Mol Biol Rep."},{"key":"B25","doi-asserted-by":"publisher","first-page":"996","DOI":"10.1095\/biolreprod.110.090126","article-title":"Adherens junction proteins in the hamster uterus: their contributions to the success of implantation","volume":"85","author":"Luan","year":"2011","journal-title":"Biol Reprod."},{"key":"B26","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1242\/jcs.00826","article-title":"Embryonic signals direct the formation of tight junctional permeability barrier in the decidualizing stroma during embryo implantation","volume":"117","author":"Wang","year":"2004","journal-title":"J Cell Sci."},{"key":"B27","doi-asserted-by":"publisher","first-page":"4349","DOI":"10.1111\/j.1742-4658.2007.05980.x","article-title":"Signaling pathways and preimplantation development of mammalian embryos","volume":"274","author":"Zhang","year":"2007","journal-title":"FEBS J."},{"key":"B28","doi-asserted-by":"publisher","first-page":"585","DOI":"10.3390\/ijms21020585","article-title":"The role of micrornas in mammalian fertility: from gametogenesis to embryo implantation","volume":"21","author":"Salilew-Wondim","year":"2020","journal-title":"Int J Mol Sci."},{"key":"B29","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1007\/s10735-019-09848-6","article-title":"Localization of Mucin 1 in endometrial luminal epithelium and its expression in women with reproductive failure during implantation window","volume":"50","author":"Wu","year":"2019","journal-title":"J Mol Histol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1580","DOI":"10.1177\/1933719116648216","article-title":"Nectin-3 is increased in the cell junctions of the uterine epithelium at implantation","volume":"23","author":"Poon","year":"2016","journal-title":"Reprod Sci."},{"key":"B31","doi-asserted-by":"publisher","first-page":"e34325","DOI":"10.1371\/journal.pone.0034325","article-title":"Junctional adhesion molecule 2 mediates the interaction between hatched blastocyst and luminal epithelium: induction by progesterone and Lif","volume":"7","author":"Su","year":"2012","journal-title":"PLoS ONE."},{"key":"B32","doi-asserted-by":"publisher","first-page":"2107","DOI":"10.1016\/j.fertnstert.2011.02.024","article-title":"Unique uterine localization and regulation may differentiate Lpa3 from other lysophospholipid receptors for its role in embryo implantation","volume":"95","author":"Ye","year":"2011","journal-title":"Fert Ster."},{"key":"B33","doi-asserted-by":"publisher","first-page":"684","DOI":"10.1016\/j.ceb.2009.06.004","article-title":"Control of cell adhesion dynamics by Rap1 signaling","volume":"21","author":"Boettner","year":"2009","journal-title":"Curr Opin Cell Biol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"1045","DOI":"10.1016\/j.prp.2018.04.007","article-title":"Rap1 promotes proliferation and migration of vascular smooth muscle cell via the ERK pathway","volume":"214","author":"Li","year":"2018","journal-title":"Pathol Res Pract."},{"key":"B35","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1007\/s10815-018-1343-x","article-title":"Extracellular vesicle mediated embryo-endometrial cross talk during implantation and in pregnancy","volume":"36","author":"Kurian","year":"2019","journal-title":"J Assist Reprod Genet."},{"key":"B36","doi-asserted-by":"publisher","first-page":"4565","DOI":"10.1002\/jcp.30180","article-title":"Exosomes from bovine endometrial epithelial cells ensure trophoblast cell development by MIR-218 targeting secreted frizzled related protein 2","volume":"236","author":"Wang","year":"2021","journal-title":"J Cell Physiol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"676791","DOI":"10.3389\/fgene.2021.676791","article-title":"Day 7 embryos change the proteomics and exosomal micro-rnas content of bovine uterine fluid: involvement of innate immune functions","volume":"12","author":"Kusama","year":"2021","journal-title":"Front Genet."},{"key":"B38","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/j.devcel.2009.06.008","article-title":"Mir-184 Has Multiple roles in drosophila female germline development","volume":"17","author":"Nicola","year":"2009","journal-title":"Dev Cell."},{"key":"B39","doi-asserted-by":"publisher","first-page":"e0122202","DOI":"10.1371\/journal.pone.0122202","article-title":"Identification and profiling of micrornas in goat endometrium during embryo implantation","volume":"10","author":"Song","year":"2015","journal-title":"PLoS ONE."},{"key":"B40","doi-asserted-by":"publisher","first-page":"1052","DOI":"10.3390\/genes11091052","article-title":"Mir-184 combined with stc2 promotes endometrial epithelial cell apoptosis in dairy goats via Ras\/Raf\/Mek\/Erk pathway","volume":"11","author":"Cui","year":"2020","journal-title":"Genes."}],"container-title":["Frontiers in Veterinary Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2022.1078394\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,20]],"date-time":"2022-12-20T04:44:35Z","timestamp":1671511475000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2022.1078394\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,20]]},"references-count":40,"alternative-id":["10.3389\/fvets.2022.1078394"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2022.1078394","relation":{},"ISSN":["2297-1769"],"issn-type":[{"value":"2297-1769","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,20]]},"article-number":"1078394"}}