{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T23:32:45Z","timestamp":1773099165017,"version":"3.50.1"},"reference-count":78,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T00:00:00Z","timestamp":1649721600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>It has long been recognized that enterotoxigenic <jats:italic>Escherichia coli<\/jats:italic> (ETEC) is the major pathogen responsible for vomiting and diarrhea. <jats:italic>E. coli<\/jats:italic> F17, a main subtype of ETEC, is characterized by high morbidity and mortality in young livestock. However, the transcriptomic basis underlying <jats:italic>E. coli<\/jats:italic> F17 infection has not been fully understood. In the present study, RNA sequencing was conducted to explore the expression profiles of mRNAs and long non-coding RNAs (lncRNAs) in the jejunum of lambs who were identified as resistant or sensitive to <jats:italic>E. coli<\/jats:italic> F17 that was obtained in a challenge experiment. A total of 772 differentially expressed (DE) mRNAs and 190 DE lncRNAs were detected between the <jats:italic>E. coli<\/jats:italic> F17\u2014resistance and <jats:italic>E. coli<\/jats:italic> F17-sensitive lambs (i.e., <jats:italic>TFF2, LOC105606142, OLFM4, LYPD8, REG4, APOA4<\/jats:italic>, TCONS_00223467, and TCONS_00241897). Then, a two-step machine learning approach (RX) combination Random Forest and Extreme Gradient Boosting were performed, which identified 16 mRNAs and 17 lncRNAs as potential biomarkers, within which <jats:italic>PPP2R3A<\/jats:italic> and TCONS_00182693 were prioritized as key biomarkers involved in <jats:italic>E. coli<\/jats:italic> F17 infection. Furthermore, functional enrichment analysis showed that peroxisome proliferator-activated receptor (PPAR) pathway was significantly enriched in response to <jats:italic>E. coli<\/jats:italic> F17 infection. Our finding will help to improve the knowledge of the mechanisms underlying <jats:italic>E. coli<\/jats:italic> F17 infection and may provide novel targets for future treatment of E. coli F17 infection.<\/jats:p>","DOI":"10.3389\/fvets.2022.819917","type":"journal-article","created":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T08:17:16Z","timestamp":1649751436000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Insights Into Long Non-Coding RNA and mRNA Expression in the Jejunum of Lambs Challenged With Escherichia coli F17"],"prefix":"10.3389","volume":"9","author":[{"given":"Weihao","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyang","family":"Lv","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weibo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingyan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiukai","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziming","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tesfaye","family":"Getachew","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joram M.","family":"Mwacharo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aynalem","family":"Haile","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,4,12]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1038\/nrmicro818","article-title":"Pathogenic Escherichia coli","volume":"2","author":"Kaper","year":"2004","journal-title":"Nat Rev Microbiol"},{"key":"B2","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1093\/infdis\/155.3.377","article-title":"Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent","volume":"155","author":"Levine","year":"1987","journal-title":"J Infect Dis."},{"key":"B3","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1016\/S0732-8893(03)00120-2","article-title":"Prevalence of enterotoxigenic Escherichia coli (ETEC) in hospitalized acute diarrhea patients in Denpasar, Bali, Indonesia","volume":"47","author":"Subekti","year":"2003","journal-title":"Diagn Microbiol Infect Dis."},{"key":"B4","doi-asserted-by":"publisher","first-page":"4697","DOI":"10.5897\/AJMR11.768","article-title":"Prevalence of the E. coli type three secretion system 2 (ETT2) locus among enterotoxigenic E. coli (ETEC) Shigatoxin-producing E coli (STEC) from weaned piglets","volume":"5","author":"Cheng","year":"2011","journal-title":"African J Microbiol Res."},{"key":"B5","first-page":"501","article-title":"The prevalence and drug resistance profile of Shiga-toxin producing (STEC), enteropathogenic (EPEC) and enterotoxigenic (ETEC) Escherichia coli in free ranging diarrheic and non-diarrheic yaks of West Kameng, Arunachal Pradesh, India","volume":"85","author":"Bandyopadhyay","year":"2015","journal-title":"Vet Arh."},{"key":"B6","doi-asserted-by":"publisher","first-page":"149","DOI":"10.3967\/bes2018.018","article-title":"Risk factors for prevalence of enterotoxigenic Escherichia coli (ETEC) in diarrheic and non-diarrheic neonatal and weaner pigs, South Africa","volume":"31","author":"Ogundare","year":"2018","journal-title":"Biomed Environ Sci."},{"key":"B7","doi-asserted-by":"publisher","first-page":"6167","DOI":"10.1016\/j.vaccine.2011.06.084","article-title":"A systematic review of ETEC epidemiology focusing on colonization factor and toxin expression","volume":"29","author":"Isidean","year":"2011","journal-title":"Vaccine."},{"key":"B8","doi-asserted-by":"publisher","first-page":"919","DOI":"10.1038\/s41385-019-0161-5","article-title":"Deficiency in intestinal epithelial Reg4 ameliorates intestinal inflammation and alters the colonic bacterial composition","volume":"12","author":"Xiao","year":"2019","journal-title":"Mucosal Immunol."},{"key":"B9","first-page":"1342","article-title":"Correlation between the presence of F5, F6, F17, F18, F41 fimbriae and the toxicity profile in Escherichia coli strains isolated from piglets with diarrhea. Medycyna Weterynaryjna-Veterinary","volume":"60","author":"Weiner","year":"2004","journal-title":"Med Sci Pract."},{"key":"B10","doi-asserted-by":"publisher","first-page":"1370","DOI":"10.1128\/JCM.37.5.1370-1375.1999","article-title":"Characterization of nonenterotoxigenic Escherichia coli strains producing F17 fimbriae isolated from diarrheic lambs and goat kids","volume":"37","author":"Cid","year":"1999","journal-title":"J Clin Microbiol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1007\/s11604-013-0270-0","article-title":"Prevalence and clinical significance of incidental F18-FDG breast uptake: a systematic review and meta-analysis","volume":"32","author":"Bertagna","year":"2014","journal-title":"Jpn J Radiol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1136\/vr.150.2.35","article-title":"Genotypic prevalence of the fimbrial adhesins (F4 F5, F6, F41 and F18) and toxins (LT, STa. STb and Stx2e) in Escherichia coli isolated from postweaning pigs with diarrhoea or oedema disease in Korea","volume":"150","author":"Kwon","year":"2002","journal-title":"Vet Record."},{"key":"B13","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1186\/s13567-014-0076-9","article-title":"Identification and detection of three new F17 fimbrial variants in Escherichia coli strains isolated from cattle","volume":"45","author":"Bihannic","year":"2014","journal-title":"Vet Res."},{"key":"B14","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1186\/s13028-020-00543-1","article-title":"Characterization of virulence genes in Escherichia coli strains isolated from pre-weaned calves in the Republic of Korea","volume":"62","author":"Ryu","year":"2020","journal-title":"Acta Vet Scand."},{"key":"B15","doi-asserted-by":"publisher","first-page":"3917","DOI":"10.1007\/s11250-020-02415-2","article-title":"Diarrheagenic Escherichia coli isolates from neonatal alpacas mainly display F17 fimbriae adhesion gene","volume":"52","author":"Siuce","year":"2020","journal-title":"Trop Anim Health Prod."},{"key":"B16","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1038\/s41580-020-00315-9","article-title":"Gene regulation by long non-coding RNAs and its biological functions","volume":"22","author":"Statello","year":"2021","journal-title":"Nat Rev Mol Cell Biol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"1780","DOI":"10.1111\/febs.14783","article-title":"A novel long non-coding RNA regulates the immune response in MAC-T cells and contributes to bovine mastitis","volume":"286","author":"Wang","year":"2019","journal-title":"FEBS J."},{"key":"B18","doi-asserted-by":"publisher","first-page":"e12525","DOI":"10.1111\/cpr.12525","article-title":"LncRNA XIST mediates bovine mammary epithelial cell inflammatory response via NF-kappaB\/NLRP3 inflammasome pathway","volume":"52","author":"Ma","year":"2019","journal-title":"Cell Prolif."},{"key":"B19","doi-asserted-by":"publisher","first-page":"16514","DOI":"10.1038\/s41598-018-34291-0","article-title":"Changes in long non-coding RNA expression profiles related to the antagonistic effects of Escherichia coli F17 on lamb spleens","volume":"8","author":"Jin","year":"2018","journal-title":"Sci Rep."},{"key":"B20","doi-asserted-by":"publisher","first-page":"907","DOI":"10.1038\/s41587-019-0201-4","article-title":"Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype","volume":"37","author":"Kim","year":"2019","journal-title":"Nat Biotechnol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie enables improved reconstruction of a transcriptome from RNA-seq reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat Biotechnol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"e166","DOI":"10.1093\/nar\/gkt646","article-title":"Utilizing sequence intrinsic composition to classify protein-coding and long non-coding transcripts","volume":"41","author":"Sun","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"B23","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1093\/nar\/gkx428","article-title":"CPC2: a fast and accurate coding potential calculator based on sequence intrinsic features","volume":"45","author":"Kang","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"B24","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1093\/nar\/gky995","article-title":"The Pfam protein families database in 2019","volume":"47","author":"El-Gebali","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"B25","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1038\/nbt.1621","article-title":"Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation","volume":"28","author":"Trapnell","year":"2010","journal-title":"Nat Biotechnol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: a Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics."},{"key":"B27","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1023\/A:1010933404324","article-title":"Random forests","volume":"45","author":"Breiman","year":"2001","journal-title":"Mach Learn."},{"key":"B28","unstructured":"xgboost: Extreme Gradient Boosting. R package version 1.5.0.1\n            ChenT\n            HeT\n            MichaelB\n            VadimK\n            TangY\n            HyunsuC\n          2016"},{"key":"B29","doi-asserted-by":"publisher","first-page":"619857","DOI":"10.3389\/fgene.2021.619857","article-title":"Identification of predictor genes for feed efficiency in beef cattle by applying machine learning methods to multi-tissue transcriptome data","volume":"12","author":"Chen","year":"2021","journal-title":"Front Genet."},{"key":"B30","doi-asserted-by":"publisher","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: a software environment for integrated models of biomolecular interaction networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res."},{"key":"B31","doi-asserted-by":"publisher","first-page":"R14","DOI":"10.1186\/gb-2010-11-2-r14","article-title":"Gene ontology analysis for RNA-seq: accounting for selection bias","volume":"11","author":"Young","year":"2010","journal-title":"Genome Biol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1093\/nar\/gkr483","article-title":"KOBAS 2.0: a web server for annotation and identification of enriched pathways and diseases","volume":"39","author":"Xie","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"B33","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods."},{"key":"B34","doi-asserted-by":"publisher","first-page":"35603","DOI":"10.18632\/oncotarget.26277","article-title":"B2M, JAK2 and MET in the genetic landscape of immunotolerance in lung cancer","volume":"9","author":"Sanchez-Cespedes","year":"2018","journal-title":"Oncotarget."},{"key":"B35","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1016\/j.canlet.2021.06.008","article-title":"Beta2-microglobulin(B2M) in cancer immunotherapies: biological function, resistance and remedy","volume":"517","author":"Wang","year":"2021","journal-title":"Cancer Lett."},{"key":"B36","doi-asserted-by":"publisher","first-page":"14524","DOI":"10.1038\/s41598-018-31719-5","article-title":"Changes in circRNA expression profiles related to the antagonistic effects of Escherichia coli F17 in lamb spleens","volume":"8","author":"Jin","year":"2018","journal-title":"Sci Rep."},{"key":"B37","doi-asserted-by":"publisher","first-page":"108048","DOI":"10.1016\/j.intimp.2021.108048","article-title":"TRIM38 protects chondrocytes from IL-1beta-induced apoptosis and degeneration via negatively modulating nuclear factor (NF)-kappaB signaling","volume":"99","author":"Hu","year":"2021","journal-title":"Int Immunopharmacol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"12286","DOI":"10.1073\/pnas.1209134109","article-title":"A forward genetic screen reveals roles for Nfkbid, Zeb1, and Ruvbl2 in humoral immunity","volume":"109","author":"Arnold","year":"2012","journal-title":"Proc Natl Acad Sci USA."},{"key":"B39","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/j.molimm.2019.07.013","article-title":"Dehydroepiandrosterone alleviates E. Coli O157:H7-induced inflammation by preventing the activation of p38 MAPK and NF-kappaB pathways in mice peritoneal macrophages","author":"Zhao","year":"2019","journal-title":"Mol Immunol 114."},{"key":"B40","doi-asserted-by":"publisher","first-page":"103568","DOI":"10.1016\/j.dci.2019.103568","article-title":"Progesterone inhibits inflammatory response in E.coli- or LPS-Stimulated bovine endometrial epithelial cells by NF-kappaB and MAPK pathways","volume":"105","author":"Cui","year":"2020","journal-title":"Dev Comp Immunol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1093\/jmcb\/mjaa003","article-title":"Enterohemorrhagic E. coli effector NleL disrupts host NF-kappaB signaling by targeting multiple host proteins","volume":"12","author":"Sheng","year":"2020","journal-title":"J Mol Cell Biol."},{"key":"B42","doi-asserted-by":"publisher","first-page":"330","DOI":"10.1186\/1471-2164-13-330","article-title":"Differential gene expression profiling of porcine epithelial cells infected with three enterotoxigenic Escherichia coli strains","volume":"13","author":"Zhou","year":"2012","journal-title":"BMC Genomics."},{"key":"B43","doi-asserted-by":"publisher","first-page":"24611","DOI":"10.1038\/srep24611","article-title":"CD14 in the TLRs signaling pathway is associated with the resistance to E. coli F18 in Chinese domestic weaned piglets","volume":"6","author":"Wu","year":"2016","journal-title":"Sci Rep."},{"key":"B44","doi-asserted-by":"publisher","first-page":"780","DOI":"10.1186\/s12864-020-07192-8","article-title":"Integrated analysis of lncRNAs and mRNAs reveals key trans-target genes associated with ETEC-F4ac adhesion phenotype in porcine small intestine epithelial cells","volume":"21","author":"Augustino","year":"2020","journal-title":"BMC Genomics."},{"key":"B45","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1038\/nri2707","article-title":"Toll-like receptor signalling in the intestinal epithelium: how bacterial recognition shapes intestinal function","volume":"10","author":"Abreu","year":"2010","journal-title":"Nat Rev Immunol."},{"key":"B46","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1515\/bmc-2015-0020","article-title":"Trefoil factor family peptides\u2013friends or foes?","volume":"6","author":"Busch","year":"2015","journal-title":"Biomol Concepts."},{"key":"B47","doi-asserted-by":"publisher","first-page":"1307","DOI":"10.1007\/s11250-019-01934-x","article-title":"Resistance to ETEC F4\/F18-mediated piglet diarrhoea: opening the gene black box","volume":"51","author":"Sinha","year":"2019","journal-title":"Trop Anim Health Prod."},{"key":"B48","doi-asserted-by":"publisher","first-page":"e0185200","DOI":"10.1371\/journal.pone.0185200","article-title":"Obstructive sleep apnea and rhonchopathy are associated with downregulation of trefoil factor family peptide 3 (TFF3)-Implications of changes in oral mucus composition","volume":"12","author":"Siber-Hoogeboom","year":"2017","journal-title":"PLoS ONE."},{"key":"B49","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1007\/s00441-018-2916-9","article-title":"Mucin 2 (MUC2) promoter characterization: an overview","volume":"374","author":"Yamashita","year":"2018","journal-title":"Cell Tissue Res."},{"key":"B50","doi-asserted-by":"publisher","first-page":"039","DOI":"10.1210\/jendso\/bvz039","article-title":"Intestinal barrier dysfunction, LPS translocation, and disease development","volume":"4","author":"Ghosh","year":"2020","journal-title":"J Endocr Soc."},{"key":"B51","doi-asserted-by":"publisher","first-page":"763","DOI":"10.3727\/096504018X15399955297355","article-title":"OLFM4 inhibits epithelial-mesenchymal transition and metastatic potential of cervical cancer cells","volume":"27","author":"Li","year":"2019","journal-title":"Oncol Res."},{"key":"B52","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1038\/s41388-021-02072-1","article-title":"OLFM4-RET fusion is an oncogenic driver in small intestine adenocarcinoma","volume":"41","author":"Liu","year":"2021","journal-title":"Oncogene."},{"key":"B53","doi-asserted-by":"publisher","first-page":"1316","DOI":"10.1093\/ibd\/izab025","article-title":"Overexpression of cancer-associated stem cell gene OLFM4 in the colonic epithelium of patients with primary sclerosing cholangitis","volume":"27","author":"Neyazi","year":"2021","journal-title":"Inflamm Bowel Dis."},{"key":"B54","doi-asserted-by":"publisher","first-page":"11056","DOI":"10.1073\/pnas.1001269107","article-title":"Olfactomedin 4 downregulates innate immunity against Helicobacter pylori infection","volume":"107","author":"Liu","year":"2010","journal-title":"Proc Natl Acad Sci USA."},{"key":"B55","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1038\/nature17406","article-title":"Lypd8 promotes the segregation of flagellated microbiota and colonic epithelia","volume":"532","author":"Okumura","year":"2016","journal-title":"Nature."},{"key":"B56","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/j.cbpa.2012.09.009","article-title":"Intestinal fatty acid binding protein (fabp2) in Atlantic salmon (Salmo salar): localization and alteration of expression during development of diet induced enteritis","volume":"164","author":"Venold","year":"2013","journal-title":"Comp Biochem Physiol A Mol Integr Physiol."},{"key":"B57","doi-asserted-by":"publisher","first-page":"1555","DOI":"10.1136\/gutjnl-2017-314759","article-title":"Increased human intestinal barrier permeability plasma biomarkers zonulin and FABP2 correlated with plasma LPS and altered gut microbiome in anxiety or depression","volume":"67","author":"Stevens","year":"2018","journal-title":"Gut."},{"key":"B58","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1556\/650.2016.30336","article-title":"Intestinal fatty acid binding protein: marker of enterocyte damage in acute and chronic gastroenterological diseases","volume":"157","author":"Kocsis","year":"2016","journal-title":"Orv Hetil."},{"key":"B59","doi-asserted-by":"publisher","first-page":"464","DOI":"10.1016\/j.dci.2016.09.007","article-title":"Analysis of apolipoprotein genes and their involvement in disease response of channel catfish after bacterial infection","volume":"67","author":"Yang","year":"2017","journal-title":"Dev Comp Immunol."},{"key":"B60","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1055\/s-2007-984466","article-title":"The satiety factor apolipoprotein A-IV modulates intestinal epithelial permeability through its interaction with alpha-catenin: Implications for inflammatory bowel diseases","volume":"39","author":"Orso","year":"2007","journal-title":"Hormone and Metabolic Research."},{"key":"B61","doi-asserted-by":"publisher","first-page":"5404","DOI":"10.7150\/jca.57880","article-title":"WFDC Protein: A promising diagnosis biomarker of ovarian cancer","volume":"12","author":"Zhang","year":"2021","journal-title":"J Cancer."},{"key":"B62","doi-asserted-by":"publisher","first-page":"68","DOI":"10.3390\/mps4040068","article-title":"Combining multiple RNA-Seq data analysis algorithms using machine learning improves differential isoform expression analysis","volume":"4","author":"Dimopoulos","year":"2021","journal-title":"Methods Protoc."},{"key":"B63","doi-asserted-by":"publisher","first-page":"40","DOI":"10.3390\/life11090940","article-title":"Identifying lung cancer cell markers with machine learning methods and single-cell RNA-Seq data","volume":"11","author":"Huang","year":"2021","journal-title":"Life."},{"key":"B64","doi-asserted-by":"publisher","first-page":"627036","DOI":"10.3389\/fimmu.2021.627036","article-title":"Single cell RNA-Seq and machine learning reveal novel subpopulations in low-grade inflammatory monocytes with unique regulatory circuits","volume":"12","author":"Lee","year":"2021","journal-title":"Front Immunol."},{"key":"B65","doi-asserted-by":"publisher","first-page":"7741","DOI":"10.1002\/cam4.2620","article-title":"Protein phosphatase 2 regulatory subunit B\u201dAlpha silencing inhibits tumor cell proliferation in liver cancer","volume":"8","author":"Chen","year":"2019","journal-title":"Cancer Med."},{"key":"B66","doi-asserted-by":"publisher","first-page":"303","DOI":"10.3389\/fmicb.2018.00303","article-title":"Integrated lung and tracheal mRNA-Seq and miRNA-Seq analysis of dogs with an avian-like H5N1 canine influenza virus infection","volume":"9","author":"Fu","year":"2018","journal-title":"Front Microbiol."},{"key":"B67","doi-asserted-by":"publisher","first-page":"646839","DOI":"10.3389\/fvets.2021.646839","article-title":"RNA-Seq analysis reveals the role of Omp16 in brucella-infected RAW264.7 cells","volume":"8","author":"Zhou","year":"2021","journal-title":"Front Vet Sci."},{"key":"B68","doi-asserted-by":"publisher","first-page":"1674","DOI":"10.3389\/fimmu.2019.01674","article-title":"Cathelicidin-WA facilitated intestinal fatty acid absorption through enhancing PPAR-gamma dependent barrier function","volume":"10","author":"Zong","year":"2019","journal-title":"Front Immunol."},{"key":"B69","doi-asserted-by":"publisher","first-page":"172974","DOI":"10.1016\/j.ejphar.2020.172974","article-title":"Gabapentin attenuates intestinal inflammation: role of PPAR-gamma receptor","volume":"873","author":"De Brito","year":"2020","journal-title":"Eur J Pharmacol."},{"key":"B70","doi-asserted-by":"publisher","first-page":"595","DOI":"10.3892\/or.2012.2154","article-title":"SLC gene-modified dendritic cells mediate T cell-dependent anti-gastric cancer immune responses in vitro","volume":"29","author":"Xue","year":"2013","journal-title":"Oncol Rep."},{"key":"B71","first-page":"71","article-title":"Expression of transporter proteins of the ABC, SLC and SLCO family in glioblastoma stem-like cells","volume":"391","author":"Bien-Moller","year":"2018","journal-title":"Arch Pharmacol."},{"key":"B72","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1155\/2020\/1932948","article-title":"The SLC family are candidate diagnostic and prognostic biomarkers in clear cell renal cell carcinoma","volume":"2020","author":"Kang","year":"2020","journal-title":"Biomed Res Int."},{"key":"B73","first-page":"456","article-title":"Glucose uptake in immune cells is lower in the presence of HIV antiretrovirals due to interactions with SLC transporters-An in vitro\/in silico investigation","volume":"178","author":"Heaton","year":"2021","journal-title":"Br J Pharmacol."},{"key":"B74","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1038\/nchembio.1429","article-title":"integrated phenotypic and activity-based profiling links Ces3 to obesity and diabetes","volume":"10","author":"Dominguez","year":"2014","journal-title":"Nat Chem Biol."},{"key":"B75","doi-asserted-by":"publisher","first-page":"2043","DOI":"10.1016\/j.bbadis.2016.08.006","article-title":"Hepatic carboxylesterase 3 (Ces3\/Tgh) is downregulated in the early stages of liver cancer development in the rat","volume":"1862","author":"Quiroga","year":"2016","journal-title":"Biochimica Et Biophysica Acta-Molecular Basis of Disease."},{"key":"B76","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1042\/BJ20050927","article-title":"Cloning and functional identification of slc5a12 as a sodium-coupled low-affinity transporter for monocarboxylates (SMCT2)","volume":"392","author":"Srinivas","year":"2005","journal-title":"Biochem J."},{"key":"B77","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1007\/s12576-018-00658-1","article-title":"Identification of the multivalent PDZ protein PDZK1 as a binding partner of sodium-coupled monocarboxylate transporter SMCT1 (SLC5A8) and SMCT2 (SLC5A12)","volume":"69","author":"Srivastava","year":"2019","journal-title":"J Physiol Sci."},{"key":"B78","doi-asserted-by":"publisher","first-page":"826","DOI":"10.1161\/CIRCRESAHA.115.304378","article-title":"Cholesterol Esters (CE) derived from hepatic sterol O-acyltransferase 2 (SOAT2) are associated with more atherosclerosis than CE from intestinal SOAT2","volume":"115","author":"Zhang","year":"2014","journal-title":"Circ Res."}],"container-title":["Frontiers in Veterinary Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2022.819917\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T08:17:31Z","timestamp":1649751451000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2022.819917\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,12]]},"references-count":78,"alternative-id":["10.3389\/fvets.2022.819917"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2022.819917","relation":{},"ISSN":["2297-1769"],"issn-type":[{"value":"2297-1769","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,12]]},"article-number":"819917"}}