{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T23:01:41Z","timestamp":1792450901336,"version":"4.5.0"},"reference-count":30,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T00:00:00Z","timestamp":1659398400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p><jats:italic>Mycoplasma bovis<\/jats:italic> (<jats:italic>M. bovis<\/jats:italic>) is one of the important pathogens for yaks. Aminoglycosides and fluoroquinolones are frequently used medications for the treatment of <jats:italic>M. bovis<\/jats:italic>. Drug-resistant strains were inevitable with the abuse of antibiotics. The resistance of <jats:italic>M. bovis<\/jats:italic> to aminoglycosides was related to the base mutations in drug target genes. Amino acid mutations at the quinolone resistance-determining region (QRDR) in gyrA, gyrB, parC, and parE conferred resistance to fluoroquinolones. In order to investigate the resistance mechanism of <jats:italic>M. bovis<\/jats:italic> from yaks in Tibet to aminoglycosides and fluoroquinolones, six frequently used antibiotics and ten clinical <jats:italic>M. bovis<\/jats:italic> strains were administered for a drug sensitivity test for <jats:italic>in vitro<\/jats:italic>-induced highly resistant strains, a drug stable-resistance test, cross-resistance test, and analysis of target gene mutations. The results showed that the clinical strains of <jats:italic>M. bovis<\/jats:italic> from yaks in Tibet had varying degrees of resistance to fluoroquinolones and aminoglycosides. The mechanism of resistance to fluoroquinolones and aminoglycosides was identified preliminarily for <jats:italic>M. bovis<\/jats:italic> from yaks: the single-site base mutation mediated the resistance of <jats:italic>M. bovis<\/jats:italic> from yaks and both base mutations led to highly resistant strains (aminoglycosides: rrs3 and rrs4; fluoroquinolones: gyrA and parC). The active efflux system results of M. bovis showed that there was no active efflux system based on fluoroquinolones and aminoglycosides expressed in <jats:italic>M. bovis<\/jats:italic> from yaks. The research could provide a reference for clinical treatment of <jats:italic>M. bovis<\/jats:italic>.<\/jats:p>","DOI":"10.3389\/fvets.2022.840981","type":"journal-article","created":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T05:23:43Z","timestamp":1659417823000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["The Resistance Mechanism of Mycoplasma bovis From Yaks in Tibet to Fluoroquinolones and Aminoglycosides"],"prefix":"10.3389","volume":"9","author":[{"given":"Jiaqiang","family":"Niu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingshuai","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhua","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yefen","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyu","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suolang","family":"Sizhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,8,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.tvjl.2007.03.008","article-title":"The role of Mycoplasma bovis in bovine respiratory disease outbreaks in veal calf feedlots","volume":"177","author":"Arcangioli","year":"2008","journal-title":"Vet J."},{"key":"B2","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1111\/j.1939-1676.2011.0750.x","volume":"25","author":"Maunsell","year":"2011","journal-title":"Mycoplasma bovis infections in cattle. 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