{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T11:45:33Z","timestamp":1768736733501,"version":"3.49.0"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T00:00:00Z","timestamp":1653955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["311182\/2017-8"],"award-info":[{"award-number":["311182\/2017-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p><jats:italic>Loxosceles<\/jats:italic> spp. (brown spiders) bites are responsible for the development of a syndrome consisting mainly of dermonecrotic lesions, and also systemic effects. Rabbits are one of the main experimental models used for better understanding the systemic and local effects of <jats:italic>Loxosceles<\/jats:italic> venom. The aim of this study is to evaluate the toxic and protective effects of rabbits immunized with <jats:italic>Loxosceles<\/jats:italic> spp. venom. Male New Zealand rabbits were allocated as a control group (CG; <jats:italic>n<\/jats:italic> = 5) that received adjuvant (Montanide) and phosphate-buffer saline (PBS), or as venom group (VG; <jats:italic>n<\/jats:italic> = 5) that received 21 \u03bcg of <jats:italic>Loxosceles<\/jats:italic> venom using Montanide as adjuvant. After five immunization cycles, a trial with 7 \u03bcg of <jats:italic>Loxosceles intermedia<\/jats:italic> (<jats:italic>L<\/jats:italic>. <jats:italic>intermedia<\/jats:italic>) venom was performed, and dermonecrotic lesions were measured. The rabbits were then euthanized, and their organs were collected for histopathology analysis. Rabbits that had undergone <jats:italic>Loxosceles<\/jats:italic> venom immunization protocol showed minor clinical disturbances during the experimental period. The used immunization protocol protected the rabbits against the toxic effect of the <jats:italic>Loxosceles<\/jats:italic> venom because they showed minor clinical disturbances during the experimental period.<\/jats:p>","DOI":"10.3389\/fvets.2022.852917","type":"journal-article","created":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T08:25:54Z","timestamp":1653985554000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Protective Effectiveness of an Immunization Protocol Against the Toxic Effects of Loxosceles intermedia Venom in Rabbits"],"prefix":"10.3389","volume":"9","author":[{"given":"Ana Lu\u00edsa Soares","family":"de Miranda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabrina de Almeida","family":"Lima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Fl\u00e1via 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