{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T05:02:58Z","timestamp":1779253378291,"version":"3.51.4"},"reference-count":71,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,5,11]],"date-time":"2022-05-11T00:00:00Z","timestamp":1652227200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>Trichuriasis is considered a neglected tropical disease, being the second most common helminthiasis in humans. Detection of <jats:italic>Trichuris<\/jats:italic> in routine diagnosis is usually done by microscopic detection of eggs in fecal samples. Other molecular analyses are more reliable and could be used, but these analyses are not routinely available in clinical microbiology laboratories. The use of matrix-assisted laser desorption\/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) is increasing since the last decades due to its recent evidence as a potential role for reliable identification of microorganisms and a few nematodes. But, for parasites detection, normalized protocols and the acquisition and introduction of new species to the database are required. We carried out a preliminary study confirming the usefulness of MALDI-TOF MS for the rapid and reliable identification of <jats:italic>Trichuris suis<\/jats:italic> used as control and the creation of an internal database. To create main spectra profiles (MSPs), the different parts of five whipworms (esophagus and intestine) were used, developing different tests to verify the repeatability and reproducibility of the spectra. Thus, to validate the new internal database, 20 whipworms, separating the esophagus and intestine, were used, of which 100% were accurately identified as <jats:italic>T. suis<\/jats:italic>, but could not distinguish between both parts of the worm. Log score values ranged between 1.84 and 2.36, meaning a high-quality identification. The results confirmed that MALDI-TOF MS was able to identify <jats:italic>Trichuris<\/jats:italic> species. Additionally, a MALDI-TOF MS profile of <jats:italic>T. suis<\/jats:italic> proteome was carried out to develop the first internal database of spectra for the diagnosis of trichuriasis and other <jats:italic>Trichuris<\/jats:italic> spp.<\/jats:p>","DOI":"10.3389\/fvets.2022.867919","type":"journal-article","created":{"date-parts":[[2022,5,11]],"date-time":"2022-05-11T19:01:24Z","timestamp":1652295684000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["The Use of MALDI-TOF MS as a Diagnostic Tool for Adult Trichuris Species"],"prefix":"10.3389","volume":"9","author":[{"given":"Julia","family":"Rivero","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Zurita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Cutillas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roc\u00edo","family":"Callej\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,5,11]]},"reference":[{"key":"B1","unstructured":"Soil-Transmitted Helminth Infections2020"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1311","DOI":"10.1172\/JCI34261","article-title":"Helminth infections: the great neglected tropical diseases","volume":"118","author":"Hotez","year":"2008","journal-title":"J Clin Invest."},{"key":"B3","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/B978-0-12-385897-9.00004-5","article-title":"Soil-transmitted helminths of humans in Southeast Asia\u2013towards integrated control","volume":"74","author":"Jex","year":"2011","journal-title":"Adv Parasitol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.vetpar.2011.05.029","article-title":"Helminth parasites in pigs: new challenges in pig production and current research highlights","volume":"180","author":"Roepstorff","year":"2011","journal-title":"Vet Parasitol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"e1539","DOI":"10.1371\/journal.pntd.0001539","article-title":"Clear genetic distinctiveness between human-and pig-derived Trichuris based on analysis of mitochondrial datasets","volume":"6","author":"Liu","year":"2012","journal-title":"PLoS Negl Trop Dis."},{"key":"B6","doi-asserted-by":"publisher","first-page":"e66249","DOI":"10.1371\/journal.pone.0066249","article-title":"Mitochondrial and nuclear ribosomal DNA evidence supports the existence of a new Trichuris species in the endangered fran\u00e7ois' leaf-monkey","volume":"8","author":"Liu","year":"2013","journal-title":"PLoS ONE."},{"key":"B7","doi-asserted-by":"publisher","first-page":"2725","DOI":"10.1007\/s00436-014-3933-6","article-title":"Trichuris colobae n. sp (Nematoda: Trichuridae), a new species of Trichuris from Colobus guereza kikuyensis","volume":"113","author":"Cutillas","year":"2014","journal-title":"Parasitol Res."},{"key":"B8","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1016\/j.meegid.2015.06.009","article-title":"Genetic heterogeneity and phylogeny of Trichuris spp. from captive non-human primates based on ribosomal DNA sequence data","volume":"34","author":"Cavallero","year":"2015","journal-title":"Infect Genet Evol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"e0004059","DOI":"10.1371\/journal.pntd.0004059","article-title":"Mitochondrial genome analysis suggest multiple Trichuris species in humans, baboons, and pigs from different geographical regions","volume":"9","author":"Hawash","year":"2015","journal-title":"PLoS Negl Trop Dis."},{"key":"B10","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.meegid.2017.04.002","article-title":"Description of a new species, Trichuris ursinus n. sp. 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