{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T02:07:57Z","timestamp":1781662077179,"version":"3.54.5"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T00:00:00Z","timestamp":1649030400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>Fish oil (FO) is an important source of lipid in functional food and aquafeeds. However, the harmful effects of oxidized fish oil (OFO) on host metabolism and reproductive health are not yet clear. In addition, lipoamide (LAM) has been widely studied as an agent for alleviating various diseases associated with oxidative disruption. Therefore, in the current study, to investigate the effects of LAM in alleviating OFO-induced decline in reproductive performance and oxidative damage to the oviduct in laying hens. We constructed a 1% fresh FO model, a 1% OFO model, and a LAM model with 1% OFO (OFO + LAM) added at 100 mg\/kg to explore the antioxidant effect of LAM. Herein, these results were evaluated by breeding performance, immune responses, estrogen, and antioxidant indices of serum samples, as well as the number of follicles and antioxidant parameters of oviducts. From the results, compared with the FO group, OFO significantly decreased the egg-laying rate, increased the contents of total protein (TP) and inflammatory factors [tumor necrosis factor \u03b1 (TNF-\u03b1), interleukin (IL)-6, IL-8, and interferon \u03b3 (INF-\u03b3)], and reduced the concentrations of anti-oxidation [total antioxidant (T-AOC), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), glutathione (GSH), glutathione reductase (GR), catalase (CAT), and hydroxyl radical scavenging activity (HRSA)] in serum samples, as well as reduced the levels of anti-oxidation indexes in oviduct tissues (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). Of note, the supplementation of LAM could significantly increase the laying performance, improve the levels of serum immunoglobulins (IgA, IgG, and IgM), serum estrogen [progesterone (P) and estradiol (E2)], and serum antioxidant parameters (T-AOC, T-SOD, GSH-Px, GSH, GR, CAT, and HRSA) and decrease the concentrations of serum inflammatory cytokines (TNF-\u03b1, IL-6, IL-8, and INF-\u03b3) in laying hens following OFO administration (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). In addition, LAM could dramatically increase the contents of antioxidant factors (<jats:italic>p<\/jats:italic> &amp;lt; 0.05) in oviducts and enhance the secretion capacity of the uterine part. Taken together, OFO caused host metabolic dysfunction, oxidative damage, uterine morphological abnormalities, and alterations of ovarian function. These results suggested that LAM administration could alleviate host metabolic dysfunctions and inflammatory damage, and then ameliorate oxidative damage in the oviduct induced by OFO, ultimately improving reproductive function.<\/jats:p>","DOI":"10.3389\/fvets.2022.875769","type":"journal-article","created":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T09:33:54Z","timestamp":1649064834000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Lipoamide Alleviates Oxidized Fish Oil-Induced Host Inflammatory Response and Oxidative Damage in the Oviduct of Laying Hens"],"prefix":"10.3389","volume":"9","author":[{"given":"Qingxiu","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiatu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimeng","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiugang","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,4,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"846","DOI":"10.1111\/rda.13432","article-title":"The impact of phytoestrogen source supplementation on reproductive performance, plasma profile, yolk fatty acids and antioxidative status in aged laying hens","volume":"54","author":"Saleh","year":"2019","journal-title":"Reprod Domest Anim."},{"key":"B2","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1080\/03079458608436310","article-title":"Death in broiler breeders due to salpingitis: possible role of zearalenone","volume":"15","author":"Bock","year":"1986","journal-title":"Avian Pathol."},{"key":"B3","first-page":"194","article-title":"The aetiology and importance of salpingitis in laying hens","volume":"14","author":"Cumming","year":"2001","journal-title":"Proc Aust Poult Sci Symp."},{"key":"B4","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1093\/jn\/70.2.199","article-title":"Toxicity of air-oxidized soybean oil","volume":"70","author":"Andrews","year":"1960","journal-title":"J Nutr."},{"key":"B5","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1093\/jn\/128.1.116","article-title":"Vitamin C supplementation restores the impaired vitamin E status of guinea pigs fed oxidized frying oil","volume":"128","author":"Liu","year":"1998","journal-title":"J Nutr."},{"key":"B6","doi-asserted-by":"publisher","first-page":"557","DOI":"10.1093\/ajcn\/70.4.557","article-title":"Molecular and cellular responses to oxidative stress and changes in oxidation-reduction imbalance in the intestine","volume":"70","author":"Aw","year":"1999","journal-title":"Am J Clin Nutr."},{"key":"B7","doi-asserted-by":"publisher","first-page":"4361","DOI":"10.3168\/jds.2006-858","article-title":"Effects of feeding oxidized fat with or without dietary antioxidants on nutrient digestibility, microbial nitrogen, and fatty acid metabolism","volume":"90","author":"V\u00e1zquez-A\u00f1\u00f3n","year":"2007","journal-title":"J Dairy Sci."},{"key":"B8","doi-asserted-by":"publisher","first-page":"208","DOI":"10.5455\/msm.2014.26.208-212","article-title":"Effects of lipoic acid on acrylamide induced testicular damage","volume":"26","author":"Lebda","year":"2014","journal-title":"Mater Sociomed."},{"key":"B9","doi-asserted-by":"publisher","first-page":"72739","DOI":"10.18632\/oncotarget.20286","article-title":"Alpha lipoic acid attenuates radiation-induced oral mucositis in rats","volume":"8","author":"Kim","year":"2017","journal-title":"Oncotarget."},{"key":"B10","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1016\/j.theriogenology.2016.09.013","article-title":"Effect of alpha lipoic acid on in vitro development of bovine secondary preantral follicles","volume":"88","author":"Zoheir","year":"2017","journal-title":"Theriogenology."},{"key":"B11","doi-asserted-by":"publisher","first-page":"8476","DOI":"10.3390\/ijms12128476","article-title":"Dietary lipoic acid influences antioxidant capability and oxidative status of broilers","volume":"12","author":"Chen","year":"2011","journal-title":"Int J Mol Sci."},{"key":"B12","doi-asserted-by":"publisher","first-page":"2809","DOI":"10.3382\/ps.2014-03921","article-title":"Effects of dietary \u03b1-lipoic acid, acetyl-l-carnitine, and sex on antioxidative ability, energy, and lipid metabolism in broilers","volume":"93","author":"Jia","year":"2014","journal-title":"Poult Sci."},{"key":"B13","doi-asserted-by":"publisher","first-page":"996","DOI":"10.5713\/ajas.2013.13436","article-title":"Effects of dietary alpha-lipoic acid and acetyl-L-carnitine on growth performance and meat quality in arbor acres broilers","volume":"27","author":"Zhang","year":"2014","journal-title":"Asian Australas J Anim Sci."},{"key":"B14","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.freeradbiomed.2005.04.002","article-title":"Exercise training and the antioxidant alpha-lipoic acid in the treatment of insulin resistance and type 2 diabetes","volume":"40","author":"Henriksen","year":"2006","journal-title":"Free Radic Biol Med."},{"key":"B15","doi-asserted-by":"publisher","first-page":"1465","DOI":"10.1016\/j.freeradbiomed.2008.01.004","article-title":"Lipoamide protects retinal pigment epithelial cells from oxidative stress and mitochondrial dysfunction","volume":"44","author":"Li","year":"2008","journal-title":"Free Radic Biol Med."},{"key":"B16","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1006\/bbrc.1998.8245","article-title":"Antioxidant reactions of dihydrolipoic acid and lipoamide with triplet duroquinone","volume":"244","author":"Bisby","year":"1998","journal-title":"Biochem Biophys Res Commun."},{"key":"B17","doi-asserted-by":"publisher","first-page":"26","DOI":"10.3164\/jcbn.11-005FR","article-title":"Lipoic acid: energy metabolism and redox regulation of transcription and cell signaling","volume":"48","author":"Packer","year":"2011","journal-title":"J Clin Biochem Nutr."},{"key":"B18","doi-asserted-by":"publisher","first-page":"e0128502","DOI":"10.1371\/journal.pone.0128502","article-title":"Lipoamide acts as an indirect antioxidant by simultaneously stimulating mitochondrial biogenesis and phase II antioxidant enzyme systems in ARPE-19 cells","volume":"10","author":"Zhao","year":"2015","journal-title":"PLoS ONE."},{"key":"B19","doi-asserted-by":"publisher","first-page":"8227","DOI":"10.1021\/acs.jafc.9b02680","article-title":"Lipoamide ameliorates oxidative stress via induction of Nrf2\/ARE signaling pathway in PC12 Cells","volume":"67","author":"Hou","year":"2019","journal-title":"J Agric Food Chem"},{"key":"B20","doi-asserted-by":"publisher","first-page":"570","DOI":"10.1016\/S0167-0115(96)02111-8","article-title":"Lipoic acid - the drug of the future?","volume":"57","author":"Bilska","year":"2005","journal-title":"Pharmacol Rep."},{"key":"B21","doi-asserted-by":"publisher","first-page":"759","DOI":"10.1007\/s12640-017-9819-5","article-title":"Alpha lipoamide ameliorates motor deficits and mitochondrial dynamics in the parkinson's disease model induced by 6-hydroxydopamine","volume":"33","author":"Zhou","year":"2018","journal-title":"Neurotox Res."},{"key":"B22","doi-asserted-by":"publisher","first-page":"1323","DOI":"10.2527\/jas.2010-3663","article-title":"Physiology and endocrinology symposium: role of the oviduct in maintaining sustained fertility in hens","volume":"89","author":"Bakst","year":"2011","journal-title":"J Anim Sci."},{"key":"B23","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.aninu.2018.09.001","article-title":"Review on docosahexaenoic acid in poultry and swine nutrition: Consequence of enriched animal products on performance and health characteristics","volume":"5","author":"Lee","year":"2019","journal-title":"Anim Nutr."},{"key":"B24","doi-asserted-by":"publisher","first-page":"459","DOI":"10.33549\/physiolres.931844","article-title":"Some current insights into oxidative stress","volume":"59","author":"Dura\u010dkov\u00e1","year":"2010","journal-title":"Physiol Res."},{"key":"B25","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1016\/j.fsi.2018.07.049","article-title":"Oxidized fish oil injury stress in Megalobrama amblycephala: Evaluated by growth, intestinal physiology, and transcriptome-based PI3K-Akt\/NF-\u03baB\/TCR inflammatory signaling","volume":"81","author":"Song","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1179\/135100001101536472","article-title":"Alpha-lipoic acid and alpha-lipoamide prevent oxidant-induced lysosomal rupture and apoptosis","volume":"6","author":"Persson","year":"2001","journal-title":"Redox Rep."},{"key":"B27","doi-asserted-by":"publisher","first-page":"1418","DOI":"10.1016\/S0891-5849(99)00014-3","article-title":"Neuroprotective effects of alpha-lipoic acid and its positively charged amide analogue","volume":"26","author":"Tirosh","year":"1999","journal-title":"Free Radic Biol Med."},{"key":"B28","doi-asserted-by":"publisher","first-page":"1475","DOI":"10.3390\/ani11051475","article-title":"Effect of dietary supplementation of fermented pine needle extract on productive performance, egg quality, and serum lipid parameters in laying hens","volume":"11","author":"Kothari","year":"2021","journal-title":"Animals."},{"key":"B29","doi-asserted-by":"publisher","first-page":"347","DOI":"10.3382\/ps.2013-03225","article-title":"Effects of dietary supplementation of quercetin on performance, egg quality, cecal microflora populations, and antioxidant status in laying hens","volume":"93","author":"Liu","year":"2014","journal-title":"Poult Sci."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1788","DOI":"10.1111\/jpn.13423","article-title":"Alpha-lipoic acid improves the reproduction performance of breeder hens during the late egg-laying period","volume":"104","author":"Dai","year":"2020","journal-title":"J Anim Physiol Anim Nutr"},{"key":"B31","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1186\/s40659-017-0123-x","article-title":"Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary","volume":"50","author":"Li","year":"2017","journal-title":"Biol Res."},{"key":"B32","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1080\/1745039X.2019.1591128","article-title":"Effects of Enterococcus faecalis on egg production, egg quality and caecal microbiota of hens during the late laying period","volume":"73","author":"Zhang","year":"2019","journal-title":"Arch Anim Nutr."},{"key":"B33","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1071\/RD17082","article-title":"Chronic stress effects and their reversibility on the Fallopian tubes and uterus in rats","volume":"30","author":"Divyashree","year":"2018","journal-title":"Reprod Fertil Dev."},{"key":"B34","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1111\/j.1439-0531.2009.01469.x","article-title":"Antioxidative effects of astaxanthin against nitric oxide-induced oxidative stress on cell viability and gene expression in bovine oviduct epithelial cell and the developmental competence of bovine IVM\/IVF embryos","volume":"45","author":"Jang","year":"2010","journal-title":"Reprod Domest Anim."},{"key":"B35","doi-asserted-by":"publisher","first-page":"996","DOI":"10.5713\/ajas.2009.90101","article-title":"Effects of dietary alpha-lipoic acid on anti-oxidative ability and meat quality in arbor acres broilers","volume":"22","author":"Zhang","year":"2009","journal-title":"Asian Australas J Anim Sci."},{"key":"B36","doi-asserted-by":"publisher","first-page":"7346","DOI":"10.1021\/jf2002393","article-title":"Lipid stability and antioxidant profile of microsomal fraction of broiler meat enriched with \u03b1-lipoic acid and \u03b1-tocopherol acetate","volume":"59","author":"Arshad","year":"2011","journal-title":"J Agric Food Chem."},{"key":"B37","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1017\/S0007114510003107","article-title":"\u03b1-Lipoic acid has anti-inflammatory and anti-oxidative properties: an experimental study in rats with carrageenan-induced acute and cotton pellet-induced chronic inflammations","volume":"105","author":"Odabasoglu","year":"2011","journal-title":"Br J Nutr."},{"key":"B38","doi-asserted-by":"publisher","first-page":"5505","DOI":"10.3390\/ijms22115505","article-title":"The effects of insulin on immortalized rat schwann cells, IFRS1","volume":"22","author":"Saiki","year":"2021","journal-title":"Int J Mol Sci."},{"key":"B39","doi-asserted-by":"publisher","first-page":"1198","DOI":"10.1038\/ni1274","article-title":"Limiting inflammatory responses during activation of innate immunity","volume":"6","author":"Han","year":"2005","journal-title":"Nat Immunol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1161\/HYPERTENSIONAHA.117.07802","article-title":"Oxidative stress, inflammation, and vascular aging in hypertension","volume":"70","author":"Guzik","year":"2017","journal-title":"Hypertension."},{"key":"B41","doi-asserted-by":"publisher","first-page":"98103","DOI":"10.18632\/oncotarget.21463","article-title":"Copper or\/and arsenic induce oxidative stress-cascaded, nuclear factor kappa B-dependent inflammation and immune imbalance, trigging heat shock response in the kidney of chicken","volume":"8","author":"Wang","year":"2017","journal-title":"Oncotarget."},{"key":"B42","doi-asserted-by":"publisher","first-page":"122","DOI":"10.3390\/biomedicines8050122","article-title":"Flavolignans from Silymarin as Nrf2 bioactivators and their therapeutic applications","volume":"8","author":"Vargas-Mendoza","year":"2020","journal-title":"Biomedicines."},{"key":"B43","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1080\/09513590.2018.1462320","article-title":"Alpha lipoic acid in obstetrics and gynecology","volume":"34","author":"Di Tucci","year":"2018","journal-title":"Gynecol Endocrinol."},{"key":"B44","doi-asserted-by":"publisher","first-page":"101465","DOI":"10.1016\/j.psj.2021.101465","article-title":"Effect of dietary serine supplementation on performance, egg quality, serum indices, and ileal mucosal immunity in laying hens fed a low crude protein diet","volume":"100","author":"Zhou","year":"2021","journal-title":"Poult Sci."},{"key":"B45","doi-asserted-by":"publisher","first-page":"9390810","DOI":"10.1155\/2018\/9390810","article-title":"Grape seed proanthocyanidin extract prevents ovarian aging by inhibiting oxidative stress in the hens","volume":"2018","author":"Liu","year":"2018","journal-title":"Oxid Med Cell Longev"},{"key":"B46","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1186\/s40104-021-00647-2","article-title":"Soya saponin improves egg-laying performance and immune function of laying hens","volume":"12","author":"Li","year":"2022","journal-title":"J Anim Sci Biotechnol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"669","DOI":"10.1080\/10715762.2019.1620938","article-title":"The effect of n-3 long-chain polyunsaturated fatty acids and lipoic acid on the heart in the ovariectomized rat model of menopause","volume":"53","author":"Marinho","year":"2019","journal-title":"Free Radic Res"},{"key":"B48","doi-asserted-by":"publisher","first-page":"188","DOI":"10.14202\/vetworld.2020.188-196","article-title":"Protective effect of alpha-lipoic acid and omega-3 fatty acids against cyclophosphamide-induced ovarian toxicity in rats","volume":"13","author":"Nair","year":"2020","journal-title":"Vet World."},{"key":"B49","doi-asserted-by":"publisher","first-page":"4378","DOI":"10.3382\/ps\/pex229","article-title":"Comparative transcriptomic analysis of high and low egg-producing duck ovaries","volume":"96","author":"Tao","year":"2017","journal-title":"Poult Sci."},{"key":"B50","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1186\/s12929-016-0253-4","article-title":"Impact of stress on oocyte quality and reproductive outcome","volume":"23","author":"Prasad","year":"2016","journal-title":"J Biomed Sci."},{"key":"B51","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1007\/s10815-011-9706-6","article-title":"The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles","volume":"29","author":"Talebi","year":"2012","journal-title":"J Assist Reprod Genet."},{"key":"B52","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1016\/j.theriogenology.2017.09.023","article-title":"Alpha lipoic acid (ALA) effects on developmental competence of equine preantral follicles in short-term culture","volume":"105","author":"Gomes","year":"2018","journal-title":"Theriogenology."}],"container-title":["Frontiers in Veterinary Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2022.875769\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T01:42:59Z","timestamp":1649814179000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2022.875769\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,4]]},"references-count":52,"alternative-id":["10.3389\/fvets.2022.875769"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2022.875769","relation":{},"ISSN":["2297-1769"],"issn-type":[{"value":"2297-1769","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,4]]},"article-number":"875769"}}