{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T17:33:30Z","timestamp":1791999210688,"version":"4.4.0"},"reference-count":53,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,4,18]],"date-time":"2022-04-18T00:00:00Z","timestamp":1650240000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>This study was conducted to investigate the alleviative effects of <jats:italic>trans<\/jats:italic>-anethole (TA) on intestinal oxidative stress by enhancing the activities of intestinal antioxidant enzymes and activating the Nrf2 signaling pathway in subclinical necrotic enteritis (NE) infected broilers. A total of 192 1-day-old male Arbor Acres broilers were randomly allocated into three treatment groups: (1) control (CON); (2) subclinical NE challenge (NE); (3) NE challenge + 600 mg\/kg TA (NE+TA600). Subclinical NE was induced by oral administration of live coccidiosis vaccine containing 2 \u00d7 10<jats:sup>4<\/jats:sup> oocysts at 10 days of age and 2 ml of <jats:italic>Clostridium perfringens<\/jats:italic> type A solution (3 \u00d7 10<jats:sup>8<\/jats:sup> CFU\/ml) daily from days 14 to 19. The results showed that NE infection led to a severe decline (<jats:italic>p<\/jats:italic> &amp;lt; 0.05) in the final body weight (BW) and average daily gain (ADG), but an increase (<jats:italic>p<\/jats:italic> &amp;lt; 0.05) in feed\/gain (F\/G) of broilers at day 10\u201321 and day 1\u201321 compared with the control group. TA administration improved (<jats:italic>p<\/jats:italic> &amp;lt; 0.05) the growth performance of NE birds. The intestinal villus height (VH) and villus height\/crypt depth (VH\/CD) were reduced (<jats:italic>p<\/jats:italic> &amp;lt; 0.05) by NE challenge as compared with those of the control group, which was elevated by TA administration. Subclinical NE infection decreased (<jats:italic>p<\/jats:italic> &amp;lt; 0.05) serum activities of total superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC), and jejunal and ileal glutathione peroxidase (GSH-PX), and T-SOD activity as well as T-AOC in the jejunum, while TA interventions positively elevated that (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). Administration of TA protected the intestine against oxidative stress through up-regulation of intestinal nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway as compared with the NE group (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). In addition, dietary inclusion of TA elevated (<jats:italic>p<\/jats:italic> &amp;lt; 0.05) mRNA abundance of c-mesenchymal-epithelial transition factor (c-Met), jejunal epidermal growth factor receptor (EGFR), and transforming growth factor-beta 1 (TGF-\u03b21) in the jejunum and ileum of birds after subclinical NE challenge. In conclusion, 600 mg\/kg of TA may be a promising tool to prevent and control subclinical NE by increasing intestinal antioxidant status in broilers.<\/jats:p>","DOI":"10.3389\/fvets.2022.877066","type":"journal-article","created":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T08:09:35Z","timestamp":1650355775000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":16,"title":["Trans-anethole Ameliorates Intestinal Injury Through Activation of Nrf2 Signaling Pathway in Subclinical Necrotic Enteritis-Induced Broilers"],"prefix":"10.3389","volume":"9","author":[{"given":"Caiyun","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichun","family":"Tong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiming","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaibin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,4,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/S0378-1135(03)00106-8","article-title":"Molecular typing of isolates of Clostridium perfringens from healthy and diseased 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