{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:29:49Z","timestamp":1787027389323,"version":"build-2736575974"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T00:00:00Z","timestamp":1653350400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31930105"],"award-info":[{"award-number":["31930105"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012453","name":"China Agricultural Research System","doi-asserted-by":"publisher","award":["CARS-40-K08"],"award-info":[{"award-number":["CARS-40-K08"]}],"id":[{"id":"10.13039\/501100012453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>\n                    Bile acids (BAs) have long been known to facilitate digestion, transport, and absorption of lipids in the small intestine as well as regulate host lipid metabolic homeostasis. However, excessive BAs may lead to long-term damage to tissue. Also, it is unknown whether different levels of porcine BAs supplementation could improve performance, host metabolism, intestinal functions in laying hens. Hence, this study was aimed to investigate the potential effects of BAs addition on laying performance, egg quality, blood parameters, organ indexes, and intestinal histopathology of hens in the late phase. A total of 300 58-week-old Hy-line Gray hens were randomly divided into five groups which fed a basal diet (control) or basal diets supplemented with 60, 300, 600, and 3,000 mg\/kg BAs for 56 days. Compared with the control group, no significant differences (\n                    <jats:italic>P<\/jats:italic>\n                    &amp;gt; 0.05) were observed in egg production, egg weight, ADFI, and FCR of hens in 60, 300, 600, and 3,000 mg\/kg BAs groups. Dietary 60 mg\/kg BAs supplementation resulted in a significant increase (\n                    <jats:italic>P<\/jats:italic>\n                    &amp;lt; 0.05) in egg mass. Meanwhile, no significant differences were observed in egg quality, including eggshell strength, eggshell thickness, albumen height, and Haugh unit among any treatment groups (\n                    <jats:italic>P<\/jats:italic>\n                    &amp;gt; 0.05). Dramatically, dietary 3,000 mg\/kg BAs supplement decreased yolk color (\n                    <jats:italic>P<\/jats:italic>\n                    &amp;lt; 0.05). There was no significant difference in the blood parameters such as WBC, RBC, HGB, HCT, and PLT among any treatments. However, in 3,000 mg\/kg BAs group, ovary coefficient was lower than the control (\n                    <jats:italic>P<\/jats:italic>\n                    &amp;lt; 0.05), and serum urea and creatinine were higher than the control (\n                    <jats:italic>P<\/jats:italic>\n                    &amp;lt; 0.05). Also, kidney and oviduct injury appeared in 3,000 mg\/kg BAs group. These results indicated that a porcine BAs concentration of 3,000 mg\/kg may cause harmful effects while 600 mg\/kg was non-deleterious to laying hens after a daily administration for 56 days, namely that dietary supplement of up to 10 times the recommended dose of BAs was safely tolerated by laying hens.\n                  <\/jats:p>","DOI":"10.3389\/fvets.2022.895831","type":"journal-article","created":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T03:44:16Z","timestamp":1653363856000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Safety Evaluation of Porcine Bile Acids in Laying Hens: Effects on Laying Performance, Egg Quality, Blood Parameters, Organ Indexes, and Intestinal Development"],"prefix":"10.3389","volume":"9","author":[{"given":"Bowen","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimeng","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shupeng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihua","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoxian","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiugang","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,5,24]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"4","DOI":"10.2174\/1389200216666151103115454","article-title":"Bile acids in physiology, pathology and pharmacology","volume":"17","author":"Marin","year":"2015","journal-title":"Curr Drug Metab."},{"key":"B2","doi-asserted-by":"publisher","first-page":"71","DOI":"10.3727\/105221618X15156018385515","article-title":"Bile acid metabolism in liver pathobiology","volume":"18","author":"Chiang","year":"2018","journal-title":"Gene Expr."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1773","DOI":"10.1053\/j.gastro.2011.07.046","article-title":"Bile acid is a host factor that regulates the composition of the cecal microbiota in rats","volume":"141","author":"Islam","year":"2011","journal-title":"Gastroenterology."},{"key":"B4","doi-asserted-by":"publisher","first-page":"S120","DOI":"10.1194\/jlr.R800026-JLR200","article-title":"Fifty years of advances in bile acid synthesis and metabolism","volume":"50","author":"Russell","year":"2009","journal-title":"J Lipid Res."},{"key":"B5","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/S0022-2275(20)42762-2","article-title":"Regulation of bile acid synthesis. 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