{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:35:51Z","timestamp":1753893351227,"version":"3.41.2"},"reference-count":67,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T00:00:00Z","timestamp":1655769600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p><jats:italic>Clostridium perfringens<\/jats:italic> beta2 (CPB2) toxin, one of the virulence factors of <jats:italic>Clostridium perfringens<\/jats:italic> (<jats:italic>C. perfringens<\/jats:italic>), can cause necrotizing enterocolitis in piglets. Accumulating pieces of evidence indicate that microRNAs (miRNAs) refer to the regulation of inflammatory processes. Previously, we have discovered that miR-30d was differentially expressed between the ileum of normal piglets and <jats:italic>C. perfringens<\/jats:italic> type C-infected diarrheal piglets. Here, we found that miR-30d expression was lowered in CPB2 toxin-treated intestinal porcine epithelial cells (IPEC-J2) at different time points. Subsequently, we determined that miR-30d inhibitor attenuated CPB2 toxin revulsive inflammatory damage in IPEC-J2 cells and promoted cell proliferation and cell cycle progression, whereas miR-30d mimic had opposite results. In addition, we confirmed that Proteasome activator subunit 3 (<jats:italic>PSME3<\/jats:italic>) was a downstream target gene of miR-30d <jats:italic>via<\/jats:italic> a dual luciferase reporter assay, qPCR, and western blot. We also found that overexpression of <jats:italic>PSME3<\/jats:italic> suppressed CPB2 toxin-induced inflammatory damage and promoted cell proliferation and cycle progression. Our results demonstrate that miR-30d aggravates CPB2 toxin revulsive IPEC-J2 cells inflammatory injury <jats:italic>via<\/jats:italic> targeting <jats:italic>PSME3<\/jats:italic>, thereby providing a novel perspective for the prevention and treatment of piglet diarrhea at the molecular level.<\/jats:p>","DOI":"10.3389\/fvets.2022.909500","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T06:57:00Z","timestamp":1655794620000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["miR-30d Inhibition Protects IPEC-J2 Cells Against Clostridium perfringens Beta2 Toxin-Induced Inflammatory 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