{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T07:49:54Z","timestamp":1780559394620,"version":"3.54.1"},"reference-count":32,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,7,20]],"date-time":"2022-07-20T00:00:00Z","timestamp":1658275200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p><jats:italic>Listeria monocytogenes<\/jats:italic> (LM) is one of the four major foodborne bacteria that cause bacteremia and meningitis. To explore the control of listeriosis with natural phages, we used the double-layer agar plate method to isolate LM from slaughterhouse sewage and designated LP8. The result of electron microscopy indicated that the phage belonged to the family of <jats:italic>Myoviridae<\/jats:italic>. Whole-genome sequencing indicated that the genome size of LP8 is 87,038 bp and contains 120 genes. Mice were infected with LM and treated with penicillin G sodium, LP8, and the combination of these two. From the levels of lymphocyte subsets (CD<jats:sup>4+<\/jats:sup>, CD<jats:sup>8+<\/jats:sup>), the expression of cytokines (TNF-\u03b1, IL1\u03b2, IL-10, and IFN-\u03b3), observation of pathological changes in organs (heart, liver, spleen, kidney, and brain), and the bacterial load of the spleen, we concluded the therapeutic effect of LP8 against listeriosis and demonstrate the feasibility of a combined therapy to reduce the use of antibiotics. This provides a new avenue for the treatment of listeriosis.<\/jats:p>","DOI":"10.3389\/fvets.2022.946814","type":"journal-article","created":{"date-parts":[[2022,8,4]],"date-time":"2022-08-04T14:09:32Z","timestamp":1659622172000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Characterization and Preliminary Application of Phage Isolated From Listeria monocytogenes"],"prefix":"10.3389","volume":"9","author":[{"given":"Tianhao","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuehui","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changqing","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumei","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huilin","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiwen","family":"Xue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huitian","family":"Gou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,7,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"944","DOI":"10.4315\/0362-028X-56.11.944","article-title":"Nisin resistance of foodborne bacteria and the specific resistance responses of Listeria monocytogenes Scott A","volume":"56","author":"Ming","year":"1993","journal-title":"J Food Prot."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1866","DOI":"10.4315\/0362-028X-70.8.1866","article-title":"An 8-year surveillance of the diversity and persistence of Listeria monocytogenes in a chilled food processing plant analyzed by amplified fragment length polymorphism","volume":"70","author":"Keto-Timonen","year":"2007","journal-title":"J Food Prot."},{"key":"B3","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1089\/fpd.2016.2274","article-title":"Listeria monocytogenes in food-processing facilities, food contamination, and human Listeriosis: the Brazilian scenario","volume":"14","author":"Camargo","year":"2017","journal-title":"Foodborne Pathog Dis."},{"key":"B4","doi-asserted-by":"publisher","first-page":"761335","DOI":"10.3389\/fcimb.2021.761335","article-title":"The DegU orphan response regulator contributes to heat stress resistance in Listeria monocytogenes","volume":"11","author":"Cheng","year":"2021","journal-title":"Front Cell Infect Microbiol"},{"key":"B5","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1111\/j.1469-0691.2009.03109.x","article-title":"Listeriosis: a resurgent foodborne infection","volume":"16","author":"Allerberger","year":"2010","journal-title":"Clin Microbiol Infect."},{"key":"B6","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/0924-2244(94)90103-1","article-title":"When are Listeria in foods a health risk?","volume":"5","author":"Hof","year":"1994","journal-title":"Trends Food Sci Technol."},{"key":"B7","first-page":"1","article-title":"Surveillance for foodborne disease outbreaks\u2014United States, 1998\u20132008","volume":"62","author":"Gould","year":"2013","journal-title":"MMWR Surveill Summ Morb Mortal Wkly Rep."},{"key":"B8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.15585\/mmwr.ss6710a1","article-title":"Surveillance for foodborne disease outbreaks \u2014 United States, 2009\u20132015","volume":"67","author":"Dewey-Mattia","year":"2018","journal-title":"MMWR Surveill Summ Morb Mortal Wkly Rep."},{"key":"B9","doi-asserted-by":"publisher","first-page":"2698","DOI":"10.3389\/fmicb.2019.02698","article-title":"Biofilm formation of Listeria monocytogenes strains under food processing environments and pan-genome-wide association study","volume":"10","author":"Lee","year":"2019","journal-title":"Front Microbiol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"1600","DOI":"10.1080\/22221751.2020.1784044","article-title":"Backtracking and forward checking of human listeriosis clusters identified a multiclonal outbreak linked to Listeria monocytogenes in meat products of a single producer","volume":"9","author":"L\u00fcth","year":"2020","journal-title":"Emerg Microbes Infect."},{"key":"B11","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1007\/s12602-010-9040-9","article-title":"Antilisterial activity on poultry meat of amylolysin, a bacteriocin from Bacillus amyloliquefaciens GA1","volume":"2","author":"Halimi","year":"2010","journal-title":"Probiotics Antimicrob Proteins."},{"key":"B12","doi-asserted-by":"publisher","first-page":"656","DOI":"10.1345\/aph.19315","article-title":"Treatment of listeriosis","volume":"34","author":"Temple","year":"2000","journal-title":"Ann Pharmacother."},{"key":"B13","doi-asserted-by":"publisher","first-page":"353","DOI":"10.33073\/pjm-2019-038","article-title":"Characteristics of Listeria monocytogenes strains isolated from milk and humans and the possibility of milk-borne strains transmission","volume":"68","author":"Skowron","year":"2019","journal-title":"Pol J Microbiol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"869339","DOI":"10.3389\/fcimb.2022.869339","article-title":"Ampicillin treatment of intracellular Listeria monocytogenes triggers formation of persistent, drug-resistant L-form cells","volume":"12","author":"Grosboillot","year":"2022","journal-title":"Front Cell Infect Microbiol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1038\/nrmicro.2017.126","article-title":"Listeria Monocytogenes: toward a complete picture of its physiology and pathogenesis","volume":"11","author":"Radoshevich","year":"2018","journal-title":"Nat Rev Microbiol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"31","DOI":"10.4161\/bact.1.1.14942","article-title":"Phages in nature","volume":"1","author":"Clokie","year":"2011","journal-title":"Bacteriophage."},{"key":"B17","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1016\/j.tim.2018.10.008","article-title":"Phage Therapy: Going Temperate?","volume":"27","author":"Monteiro","year":"2019","journal-title":"Trends Microbiol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1007\/978-1-4939-9877-7_4","article-title":"Bacteriophage isolation and characterization: phages of Escherichia coli","volume":"2075","author":"Jofre","year":"2020","journal-title":"Methods Mol Biol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"110492","DOI":"10.1016\/j.foodres.2021.110492","article-title":"Application of a novel phage vB_SalS-LPSTLL for the biological control of Salmonella in foods","volume":"147","author":"Guo","year":"2021","journal-title":"Food Res Int."},{"key":"B20","first-page":"60","article-title":"Long-term preservation of unstable bacteriophages of enterobacteria","volume":"74","author":"Tovkach","year":"2012","journal-title":"Mikrobiol Z."},{"key":"B21","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1038\/s41579-019-0311-5","article-title":"Phage diversity, genomics and phylogeny","volume":"18","author":"Dion","year":"2020","journal-title":"Nat Rev Microbiol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"3987","DOI":"10.2174\/0929867324666170413100136","article-title":"Phage life cycles behind bacterial biodiversity","volume":"24","author":"Olszak","year":"2017","journal-title":"Curr Med Chem."},{"key":"B23","doi-asserted-by":"publisher","first-page":"324","DOI":"10.3934\/microbiol.2019.4.324","article-title":"Bacteriophages\u2014a new hope or a huge problem in the food industry","volume":"5","author":"Poaska","year":"2019","journal-title":"AIMS Microbiol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"103239","DOI":"10.1016\/j.fm.2019.06.003","article-title":"Cross-resistance to phage infection in Listeria monocytogenes serotype 1\/2a mutants","volume":"84","author":"Trudelle","year":"2019","journal-title":"Food Microbiol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"671","DOI":"10.3390\/v13040671","article-title":"Characterization of a novel group of Listeria phages that target serotype 4b Listeria monocytogenes","volume":"13","author":"Song","year":"2021","journal-title":"Viruses"},{"key":"B26","doi-asserted-by":"publisher","first-page":"320","DOI":"10.5851\/kosfa.2017.37.2.320","article-title":"Isolation and characterization of listeria phages for control of growth of Listeria monocytogenes in milk. Hangug Chugsan Sigpum Haghoeji = Korean","volume":"37","author":"Lee","year":"2017","journal-title":"J Food Sci Anim Resour."},{"key":"B27","doi-asserted-by":"publisher","first-page":"3819","DOI":"10.1128\/JCM.42.8.3819-3822.2004","article-title":"Differentiation of the major Listeria monocytogenes serovars by multiplex PCR","volume":"42","author":"Doumith","year":"2004","journal-title":"J Clin Microbiol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"a1869","DOI":"10.4102\/ojvr.v87i1.1869","article-title":"Review of Listeria monocytogenes from meat and meat products: epidemiology, virulence factors, antimicrobial resistance and diagnosis","volume":"87","author":"Matle","year":"2020","journal-title":"Onderstepoort J Vet Res."},{"key":"B29","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.ijmm.2010.05.002","article-title":"Listeria monocytogenes lineages: Genomics, evolution, ecology, and phenotypic characteristics","volume":"301","author":"Orsi","year":"2011","journal-title":"Int J Med Microbiol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"e00066","DOI":"10.1128\/CMR.00066-18","article-title":"Phage therapy in the postantibiotic era","volume":"16","author":"Gordillo Altamirano","year":"2019","journal-title":"Clin Microbiol Rev."},{"key":"B31","doi-asserted-by":"publisher","first-page":"182","DOI":"10.3390\/antibiotics8040182","article-title":"Phage-Antibiotic Combination Treatments: Antagonistic Impacts of Antibiotics on the Pharmacodynamics of Phage Therapy?","volume":"11","author":"Abedon","year":"2019","journal-title":"Antibiotics."},{"key":"B32","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1039\/C5IB00267B","article-title":"MacDonald PR, Jaramillo A. Genetically modified bacteriophages","volume":"18","author":"Sagona","year":"2016","journal-title":"Integr Biol."}],"container-title":["Frontiers in Veterinary Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2022.946814\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T16:33:39Z","timestamp":1663864419000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2022.946814\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,20]]},"references-count":32,"alternative-id":["10.3389\/fvets.2022.946814"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2022.946814","relation":{},"ISSN":["2297-1769"],"issn-type":[{"value":"2297-1769","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,20]]},"article-number":"946814"}}