{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T22:38:25Z","timestamp":1783723105152,"version":"3.55.0"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T00:00:00Z","timestamp":1658188800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2022-ZJ-956Q"],"award-info":[{"award-number":["2022-ZJ-956Q"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>Neosporosis is a worldwide infectious disease caused by intracellular parasite <jats:italic>Neospora caninum<\/jats:italic> that is a major pathogen of abortion in cattle and neurological disorders in other hosts. However, limited data are available on animals exposed to <jats:italic>N. caninum<\/jats:italic> in the Qinghai-Tibetan Plateau Area (QTPA), and little is known about whether animals in the plateau area play an important role in the epidemiology of <jats:italic>N. caninum<\/jats:italic>. Therefore, indirect ELISAs based on a combination of <jats:italic>Nc<\/jats:italic>SAG1 and <jats:italic>Nc<\/jats:italic>GRA7 antigens were developed to examine both <jats:italic>N. caninum<\/jats:italic>-specific IgG and IgM antibodies in Tibetan sheep, yak, cow, pig, cattle, horse, chicken, camel, and donkey from the QTPA in this study. The results showed that all current species present- IgG and IgM-positive animals, and that the overall seroprevalence of <jats:italic>N. caninum<\/jats:italic> were 18.6 (703\/3,782) and 48.1% (1,820\/3,782) for the IgG and IgM antibodies, respectively. Further analysis found significant differences from different altitudes in IgG in Tibetan sheep and IgM in the yak. Hence, the present serological results indicate that the tested animal populations in the QTPA are suffering from <jats:italic>N. caninum<\/jats:italic> infections or have become carriers of <jats:italic>N. caninum<\/jats:italic> antibodies. To the best of our knowledge, this is the first report on current <jats:italic>N. caninum<\/jats:italic>-infected animals in the QTPA, the first epidemiology of neosporosis in cow and camel in China, and the first record of <jats:italic>N. caninum<\/jats:italic> IgM antibodies in all the surveyed animals in China. This study provides the latest valuable data on the epidemiology of neosporosis in China and in plateau areas of the world.<\/jats:p>","DOI":"10.3389\/fvets.2022.953380","type":"journal-article","created":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T06:11:54Z","timestamp":1658211114000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["Seroepidemiology of Neosporosis in Various Animals in the Qinghai-Tibetan 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