{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:28:27Z","timestamp":1792092507695,"version":"4.4.0"},"reference-count":25,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T00:00:00Z","timestamp":1667520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>Canine brucellosis is primarily caused by <jats:italic>Brucella canis<\/jats:italic>, but other <jats:italic>Brucella<\/jats:italic> species can also cause the disease. Identifying sequences specific to <jats:italic>B. canis<\/jats:italic> and establishing PCR assays that can distinguish between <jats:italic>B. canis<\/jats:italic> and other <jats:italic>Brucella<\/jats:italic> species is essential to determine the etiology of canine brucellosis and the source of infection and to achieve effective control. We analyzed the gaps and SNPs of genomes I and II from <jats:italic>B. canis<\/jats:italic> strain RM6\/66 and <jats:italic>B. melitensis<\/jats:italic> strain 16M using the Mauve genome alignment software, and the specificity of each of these differential regions was analyzed by BLAST. A 132 bp specific sequence was found between the DK60_915 (glycosyl hydrolase 108 family protein) and DK60_917 (aldose 1-epimerase) loci in <jats:italic>B. canis<\/jats:italic> chromosome 1. Further comparative analysis revealed that this is a reverse complement sequence between <jats:italic>B. canis<\/jats:italic> and other <jats:italic>Brucella<\/jats:italic> species. Then, three primers were designed based on the sequence that could detect <jats:italic>B. canis<\/jats:italic> with a 310 bp amplification product or other <jats:italic>Brucella<\/jats:italic> species with a 413 bp product. The PCR based on these primers had reasonable specificity and a sensitivity of 100 copies of <jats:italic>Brucella<\/jats:italic> DNA. The detection results for the blood samples of the aborted dogs showed a favorable accordance with the Bruce-ladder multiplex PCR assay. In conclusion, we found a specific reverse complement sequence between <jats:italic>B. canis<\/jats:italic> and other <jats:italic>Brucella<\/jats:italic> and developed a PCR method that allows a more comprehensive identification of the pathogen involved in canine brucellosis. These findings provide an effective means for preventing and controlling brucellosis.<\/jats:p>","DOI":"10.3389\/fvets.2022.983482","type":"journal-article","created":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T06:18:16Z","timestamp":1667542696000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["A specific reverse complement sequence for distinguishing Brucella canis from other Brucella 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