{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:36:29Z","timestamp":1753893389015,"version":"3.41.2"},"reference-count":66,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T00:00:00Z","timestamp":1687305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>Over the past 10 years, immunization of cattle in Russia has been performed using vaccines from <jats:italic>Brucella abortus<\/jats:italic> strains 82, 19 and 75\/79. To prevent brucellosis in small ruminants, two vaccines have been used, from the <jats:italic>Brucella melitensis<\/jats:italic> strain REV-1 and the <jats:italic>B. abortus<\/jats:italic> strain 19; note that twice as many animals have been immunized with the former vaccine than with the latter vaccine. The disadvantage of using these preparations is the formation of prolonged post-vaccination seropositivity, which is especially pronounced in animals after immunization with vaccines from <jats:italic>B. abortus<\/jats:italic> strain 19 and <jats:italic>B. melitensis<\/jats:italic> strain REV-1. This study aims to perform the whole genome sequencing of <jats:italic>Brucella<\/jats:italic> vaccine strains from the Russian collection. A bioinformatics analysis of the genomic data proved that the vaccine strains 75\/79AB, 82, R-1096, and the KV 17\/100 belong to ST-2, 104 M to ST-1, KV 13\/100 to ST-5. This analysis allowed us to characterize vaccine strains\u2019s phylogenetic relationships and to prove the close relation of vaccine strains 75\/79AB, 82, R-1096. Also, we defined candidate mutations in genes <jats:italic>pmm, wbdA, wbkA, wboA,<\/jats:italic> and <jats:italic>eryB<\/jats:italic>, which could be responsible for the attenuated virulence of vaccine strains. The complete genomic sequences of <jats:italic>B. abortus<\/jats:italic> strains make further studies of bacterial pathogenicity determinants and virulence phenotype feasible, as well as their use in quality control of animal medicines.<\/jats:p>","DOI":"10.3389\/fvets.2023.1154520","type":"journal-article","created":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T12:45:23Z","timestamp":1687351523000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Russian collection of Brucella abortus vaccine strains: annotation, implementation and genomic analysis"],"prefix":"10.3389","volume":"10","author":[{"given":"Olga","family":"Prasolova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekaterina","family":"Krylova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Bogomazova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina","family":"Soltynskaya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oleg","family":"Sklyarov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronika","family":"Gordeeva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina","family":"Timofeeva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anton","family":"Motorygin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Panin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2023,6,21]]},"reference":[{"key":"ref1","first-page":"103","volume-title":"The 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Histopathologic appraisal of immunity induced in mice by a streptomycin-dependent mutant of Brucella melitensis","volume":"31","author":"Elberg","year":"1955","journal-title":"Am J Pathol"},{"key":"ref49","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/0378-1135(91)90091-S","article-title":"Biological properties of RB51; a stable rough strain of Brucella abortus","volume":"28","author":"Schurig","year":"1991","journal-title":"Vet Microbiol"},{"key":"ref50","first-page":"42","article-title":"Biological properties of the vaccine strain B. abortus 104 M","volume":"57","author":"Shumilov","year":"1983","journal-title":"Proc VIEV"},{"key":"ref51","first-page":"43","article-title":"Live vaccine against brucellosis from strain 82","author":"Salmakov","year":"1975","journal-title":"Vet Med"},{"year":"1997","author":"Nikiforov","key":"ref52"},{"year":"1999","author":"Kalmykov","key":"ref53"},{"year":"2000","author":"Kalmykov","key":"ref54"},{"year":"1979","author":"Salmakov","key":"ref55"},{"key":"ref56","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1080\/01652176.2020.1868616","article-title":"Bovine brucellosis\u2014a comprehensive review","volume":"41","author":"Khurana","year":"2021","journal-title":"Vet Q"},{"year":"","key":"ref57"},{"key":"ref58","doi-asserted-by":"publisher","first-page":"F31","DOI":"10.1016\/j.vaccine.2010.03.049","article-title":"Designing vaccines against cattle brucellosis","volume":"28","author":"Shumilov","year":"2010","journal-title":"Vaccine"},{"key":"ref59","doi-asserted-by":"publisher","first-page":"392","DOI":"10.1016\/j.vetmic.2009.06.021","article-title":"Brucellosis: a re-emerging zoonosis","volume":"140","author":"Seleem","year":"2009","journal-title":"Vet Microbiol"},{"key":"ref60","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1016\/j.molimm.2015.04.004","article-title":"A Brucella melitensis M5-90 wboA deletion strain is attenuated and enhances vaccine efficacy","volume":"66","author":"Li","year":"2015","journal-title":"Mol Immunol"},{"key":"ref61","doi-asserted-by":"publisher","first-page":"3812","DOI":"10.1093\/nar\/gkg509","article-title":"SIFT: predicting amino acid changes that affect protein function","volume":"31","author":"Ng","year":"2003","journal-title":"Nucleic Acids Res"},{"key":"ref62","doi-asserted-by":"publisher","first-page":"D204","DOI":"10.1093\/nar\/gku989","article-title":"UniProt: a hub for protein information","volume":"43","year":"2015","journal-title":"Nucleic Acids Res"},{"key":"ref63","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0378-1097(03)00963-7","article-title":"Brucella pathogenesis, genes identified from random large-scale screens","volume":"231","author":"Delrue","year":"2004","journal-title":"FEMS Microbiol Lett"},{"key":"ref64","doi-asserted-by":"publisher","first-page":"760","DOI":"10.1128\/CDLI.6.5.760-764.1999","article-title":"Identification of an IS 711 element interrupting the wboA gene of Brucella abortus vaccine strain RB51 and a PCR assay to distinguish strain RB51 from other Brucella species and strains","volume":"6","author":"Vemulapalli","year":"1999","journal-title":"Clin Diagn Lab Immunol"},{"key":"ref65","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1099\/00221287-146-2-487","article-title":"The genes for erythritol catabolism are organized as an inducible operon in Brucella abortus","volume":"146","author":"Sangari","year":"2000","journal-title":"Microbiology"},{"key":"ref66","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1111\/j.1574-6968.1994.tb07123.x","article-title":"The Brucella abortus vaccine strain B19 carries a deletion in the erythritol catabolic genes","volume":"121","author":"Sangari","year":"1994","journal-title":"FEMS Microbiol Lett"}],"container-title":["Frontiers in Veterinary Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2023.1154520\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T12:45:30Z","timestamp":1687351530000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2023.1154520\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,21]]},"references-count":66,"alternative-id":["10.3389\/fvets.2023.1154520"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2023.1154520","relation":{},"ISSN":["2297-1769"],"issn-type":[{"type":"electronic","value":"2297-1769"}],"subject":[],"published":{"date-parts":[[2023,6,21]]},"article-number":"1154520"}}