{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T03:52:43Z","timestamp":1792727563673,"version":"4.5.0"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T00:00:00Z","timestamp":1685923200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>\n                    <jats:italic>Vibrio splendidus<\/jats:italic>\n                    is an opportunistic pathogen that causes skin ulcer syndrome and results in huge losses to the\n                    <jats:italic>Apostichopus japonicus<\/jats:italic>\n                    breeding industry. Ferric uptake regulator (Fur) is a global transcription factor that affects varieties of virulence-related functions in pathogenic bacteria. However, the role of the\n                    <jats:italic>V. splendidus fur<\/jats:italic>\n                    (\n                    <jats:italic>Vsfur<\/jats:italic>\n                    ) gene in the pathogenesis of\n                    <jats:italic>V. splendidus<\/jats:italic>\n                    remains unclear. Hence, we constructed a\n                    <jats:italic>Vsfur<\/jats:italic>\n                    knock-down mutant of the\n                    <jats:italic>V. splendidus<\/jats:italic>\n                    strain (MTVs) to investigate the role of the gene in the effect of biofilm, swarming motility, and virulence on\n                    <jats:italic>A. japonicus<\/jats:italic>\n                    . The result showed that the growth curves of the wild-type\n                    <jats:italic>V. splendidus<\/jats:italic>\n                    strain (WTVs) and MTVs were almost consistent. Compared with WTVs, the significant increases in the transcription of the virulence-related gene\n                    <jats:italic>Vshppd<\/jats:italic>\n                    mRNA were 3.54- and 7.33-fold in MTVs at the OD\n                    <jats:sub>600<\/jats:sub>\n                    of 1.0 and 1.5, respectively. Similarly, compared with WTVs, the significant increases in the transcription of\n                    <jats:italic>Vsm<\/jats:italic>\n                    mRNA were 2.10- and 15.92-fold in MTVs at the OD\n                    <jats:sub>600<\/jats:sub>\n                    of 1.0 and 1.5, respectively. On the contrary, the mRNA level of the flagellum assembly gene\n                    <jats:italic>Vsflic<\/jats:italic>\n                    was downregulated 0.56-fold in MTVs at the OD\n                    <jats:sub>600<\/jats:sub>\n                    of 1.0 compared with the WTVs. MTVs caused delayed disease onset time and reduced\n                    <jats:italic>A. japonicus<\/jats:italic>\n                    mortality. The median lethal doses of WTVs and MTVs were 9.116\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    and 1.658\u2009\u00d7\u200910\n                    <jats:sup>11<\/jats:sup>\n                    \u2009CFU\u00b7ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    , respectively. Compared with WTVs, the colonization abilities of MTVs to the muscle, intestine, tentacle, and coelomic fluid of\n                    <jats:italic>A. japonicus<\/jats:italic>\n                    were significantly reduced. Correspondingly, the swarming motility and biofilm formation in normal and iron-replete conditions were remarkably decreased compared with those of WTVs. Overall, these results demonstrate that Vsfur contributes to the pathogenesis of\n                    <jats:italic>V. splendidus<\/jats:italic>\n                    by regulating virulence-related gene expression and affecting its swarming and biofilm formation abilities.\n                  <\/jats:p>","DOI":"10.3389\/fvets.2023.1207831","type":"journal-article","created":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T00:36:42Z","timestamp":1685925402000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Vibrio splendidus Fur regulates virulence gene expression, swarming motility, and biofilm formation, affecting its pathogenicity in Apostichopus japonicus"],"prefix":"10.3389","volume":"10","author":[{"given":"Yue","family":"Shi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changyu","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fa","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenghua","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weikang","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,6,5]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"139","DOI":"10.3354\/dao046139","article-title":"A Vibrio 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