{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T20:53:06Z","timestamp":1759179186304,"version":"3.41.2"},"reference-count":49,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T00:00:00Z","timestamp":1704326400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p><jats:italic>Staphylococcus coagulans<\/jats:italic> (SC) belongs to a group of coagulase-positive staphylococci occasionally isolated from the skin lesions of dogs with pyoderma. We recently revealed that erythritol, a sugar alcohol, inhibited the growth of SC strain JCM7470. This study investigated the molecular mechanisms involved in this growth inhibition of JCM7470 by erythritol, and determine whether erythritol inhibits the growth of SC isolated from the skin of dogs with pyoderma. Comprehensive analysis of the gene expression of JCM7470 in the presence of erythritol revealed that erythritol upregulated the expression of <jats:italic>glcB<\/jats:italic> and <jats:italic>ptsG<\/jats:italic> genes, both of which encode phosphotransferase system (PTS) glucoside- and glucose-specific permease C, B, and A domains (EIICBA), respectively, associated with sugar uptake. Moreover, erythritol suppressed <jats:italic>in vitro<\/jats:italic> growth of all 27 SC strains isolated from the skin lesions of canine pyoderma, including 13 <jats:italic>mecA<\/jats:italic> gene-positive and 14 <jats:italic>mecA<\/jats:italic> gene-negative strains. Finally, the growth inhibition of the SC clinical isolates by erythritol was restored by the addition of glucose. In summary, we revealed that erythritol promotes PTS gene expression and suppresses the <jats:italic>in vitro<\/jats:italic> growth of SC clinical isolates from dogs with pyoderma. Restoration of the erythritol-induced growth inhibition by glucose suggested that glucose starvation may contribute to the growth inhibition of SC.<\/jats:p>","DOI":"10.3389\/fvets.2023.1272595","type":"journal-article","created":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T05:02:50Z","timestamp":1704344570000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Erythritol alters phosphotransferase gene expression and inhibits the in vitro growth of Staphylococcus coagulans isolated from canines with pyoderma"],"prefix":"10.3389","volume":"10","author":[{"given":"Saki","family":"Onishi-Sakamoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadashi","family":"Fujii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keito","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reina","family":"Makida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keita","family":"Iyori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoichi","family":"Toyoda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takumi","family":"Tochio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Nishifuji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2024,1,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"5926","DOI":"10.1099\/ijsem.0.004498","article-title":"Phylogenomic analyses of the Staphylococcaceae family suggest the reclassification of five species within the genus Staphylococcus as heterotypic synonyms, the promotion of five subspecies to novel species, the taxonomic reassignment of five Staphylococcus species to Mammaliicoccus gen. nov, and the formal assignment of Nosocomiicoccus to the family Staphylococcaceae","volume":"70","author":"Madhaiyan","year":"2020","journal-title":"Int J Syst Evol Microbiol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1099\/00207713-40-4-409","article-title":"Staphylococcus schleiferi subsp. coagulans subsp nov, isolated from the external auditory meatus of dogs with external ear otitis","volume":"40","author":"Igimi","year":"1990","journal-title":"Int J Syst Bacteriol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1136\/vr.150.15.487","article-title":"Isolation of Staphylococcus schleiferi subspecies coagulans from two cases of canine pyoderma","volume":"150","author":"Bes","year":"2002","journal-title":"Vet Rec."},{"key":"B4","doi-asserted-by":"publisher","first-page":"451","DOI":"10.2460\/javma.2003.222.451","article-title":"Isolation of Staphylococcus schleiferi from dogs with pyoderma","volume":"222","author":"Frank","year":"2003","journal-title":"J Am Vet Med Assoc."},{"key":"B5","doi-asserted-by":"publisher","first-page":"928","DOI":"10.2460\/javma.2005.227.928","article-title":"Isolation of Staphylococcus schleiferi from healthy dogs and dogs with otitis, pyoderma, or both","volume":"227","author":"May","year":"2005","journal-title":"J Am Vet Med Assoc."},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.04670-22","article-title":"Molecular characterization and clinical relevance of taxonomic reassignment of Staphylococcus schleiferi Subspecies into two separate species, Staphylococcus schleiferi and Staphylococcus coagulans","author":"Naing","year":"2023","journal-title":"Microbiol Spectr"},{"key":"B7","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/j.tvjl.2018.04.002","article-title":"What has changed in canine pyoderma? 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