{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:36:20Z","timestamp":1753893380367,"version":"3.41.2"},"reference-count":19,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T00:00:00Z","timestamp":1696809600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>Worldwide, various counting chambers and computer-assisted sperm analysis (CASA) devices are in use. The semen\u2019s concentration can vary depending on the depth of the counting chamber and how it is loaded. The study\u2019s objectives were to analyze the effects of various counting chambers on semen concentration results using a GoldCyto\u00ae slide and a glass slide in the CASA system and to ascertain the precision of concentration measurements made using glass slides on CASA. The study\u2019s control group was composed of samples with known concentrations (72\u201380 million sperm\/mL) as determined by a spectrophotometer. A total of 21 frozen straws from the same bull of the same date were thawed at 37\u00b0C for 30\u2009s and loaded into two different sperm-counting chambers (GoldCyto\u00ae slide and glass slide). The sample semen placed in the sperm counting chambers was 5\u2009\u03bcL and the same value was entered in the CASA software as 5\u2009\u03bcL. Measurements were done and evaluated in 5 different areas. According to the data we obtained, using the glass slide were statistically lower than the spectrophotometer (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.001). GoldCyto\u00ae slide results were consistent with spectrophotometer results. Consequently, measurements with GoldCyto\u00ae slides in the CASA had consistent results, while measurements with glass slides were inconsistent. It was concluded that GoldCyto\u00ae slides are more suitable than glass slides in the concentration examinations of semen. Therefore, more study is needed to optimize the use of glass slides.<\/jats:p>","DOI":"10.3389\/fvets.2023.1283128","type":"journal-article","created":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T10:41:27Z","timestamp":1696848087000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of sperm counting accuracy on computer-assisted sperm analysis with GoldCyto\u00ae slides and glass slides"],"prefix":"10.3389","volume":"10","author":[{"given":"Eser","family":"Akal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2023,10,9]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1038\/aja.2009.14","article-title":"Clinical significance of the low normal sperm morphology value as proposed in the fifth edition of the WHO laboratory manual for the examination and processing of human semen","volume":"12","author":"Menkveld","year":"2010","journal-title":"Asian J Androl"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"e13614","DOI":"10.1111\/and.13614","article-title":"Diagnostic value of routine semen analysis in clinical andrology","volume":"53","author":"Baskaran","year":"2021","journal-title":"J Androl"},{"key":"ref3","doi-asserted-by":"crossref","DOI":"10.1007\/978-981-10-5200-2","volume-title":"Protocols in semen biology (comparing assays)","author":"Srivastava","year":"2017"},{"volume-title":"WHO laboratory manual for the examination and processing of human semen","year":"2010","key":"ref4"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"614","DOI":"10.1016\/j.theriogenology.2005.05.047","article-title":"Sperm concentration determination between hemacytometric and CASA systems: Why they can be different","volume":"64","author":"Kuster","year":"2005","journal-title":"Theriogenology"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1016\/s0015-0282(97)81526-9","article-title":"The performance of 10 different methods for the estimation of sperm concentration","volume":"68","author":"Mahmoud","year":"1997","journal-title":"Fertil Steril"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"257","DOI":"10.2164\/jandrol.05112","article-title":"The precision and accuracy of six different methods to determine sperm concentration","volume":"27","author":"Prathalingam","year":"2006","journal-title":"J Androl"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"661","DOI":"10.2164\/jandrol.108.007500","article-title":"Comparison of different methods for assessment of sperm concentration and membrane integrity with bull semen","volume":"30","author":"Anzar","year":"2009","journal-title":"J Androl"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"1507","DOI":"10.1016\/j.theriogenology.2016.01.002","article-title":"Andrology laboratory review: Evaluation of sperm concentration","volume":"85","author":"Brito","year":"2016","journal-title":"Theriogenology"},{"key":"ref10","first-page":"37","article-title":"NAAB-CSS semen quality control program minimum guidelines","volume-title":"Proceedings of the 24th Technical Conference on Artificial Insemination and Reproduction","author":"Brito","year":"2012"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.5713\/ab.21.0072","article-title":"Semen evaluation: Methodological advancements in sperm quality-specific fertility assessment-A review","volume":"34","author":"Tanga","year":"2021","journal-title":"Anim Biosci"},{"key":"ref12","first-page":"3","article-title":"Methods for sperm concentration determination","volume-title":"Spermatogenesis. methods in molecular biology","author":"Bj\u00f6rndahl","year":"2013"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1080\/01485010290099147","article-title":"Quality control, quality assurance, and proficiency testing in the andrology laboratory","volume":"48","author":"Keel","year":"2002","journal-title":"Arch Androl"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1007\/s10815-005-7461-2","article-title":"External quality control program for semen analysis: Spanish experience","volume":"22","author":"Alvarez","year":"2005","journal-title":"J Assist Reprod Genet"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1071\/rd17551","article-title":"CASA-Mot technology: how results are affected by the frame rate and counting chamber","volume":"30","author":"Bompart","year":"2018","journal-title":"Reprod Fertil Dev"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1017\/s002211206200110x","article-title":"Behaviour of macroscopic rigid spheres in Poiseuille flow Part 1. Determination of local concentration by statistical analysis of particle passages through crossed light beams","volume":"14","author":"Segre","year":"1962","journal-title":"J Fluid Mech"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1111\/j.2047-2927.2012.00042.x","article-title":"High variability in results of semen analysis in andrology laboratories in Tuscany (Italy): The experience of an external quality control (EQC) programme","volume":"1","author":"Filimberti","year":"2013","journal-title":"Andrology"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1080\/19396368.2020.1850907","article-title":"Comparison of commercially available chamber slides for computer-aided analysis of human sperm","volume":"67","author":"Dardmeh","year":"2020","journal-title":"Syst Biol Reprod Med"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"383","DOI":"10.2527\/jas.2010-3056","article-title":"A comparison of bovine seminal quality assessments using different viewing chambers with a computer-assisted semen analyzer","volume":"89","author":"Lenz","year":"2011","journal-title":"J Anim Sci"}],"container-title":["Frontiers in Veterinary Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2023.1283128\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T10:41:30Z","timestamp":1696848090000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2023.1283128\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,9]]},"references-count":19,"alternative-id":["10.3389\/fvets.2023.1283128"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2023.1283128","relation":{},"ISSN":["2297-1769"],"issn-type":[{"type":"electronic","value":"2297-1769"}],"subject":[],"published":{"date-parts":[[2023,10,9]]},"article-number":"1283128"}}