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This mechanism naturally picks high-quality sperm cells for conception, but when artificial reproductive technologies such as <jats:italic>in vitro<\/jats:italic> fertilization, intracytoplasmic sperm injection, or intrauterine insemination are utilized, alternative techniques of sperm selection are necessary. Currently, technology allows for sperm sorting based on motility, maturity, the lack of apoptotic components, proper morphology, and even sex. This study provides current knowledge on all known techniques of sperm cell sorting in buffaloes, evaluates their efficiency, and discusses the benefits and drawbacks of each approach.<\/jats:p>","DOI":"10.3389\/fvets.2023.1347482","type":"journal-article","created":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T04:40:47Z","timestamp":1704861647000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["New insights into semen separation techniques in buffaloes"],"prefix":"10.3389","volume":"10","author":[{"given":"Crina Raluca","family":"Andrei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florin Petri\u0219or","family":"Posastiuc","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolae 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