{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T16:50:37Z","timestamp":1778172637899,"version":"3.51.4"},"reference-count":22,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T00:00:00Z","timestamp":1712534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>This study aimed to determine the correlation of the parameters that indicate the status of the ocular surface with the prognosis of corneal opacification. Fifty dogs (96 eyes) were examined using a grid-line illuminator (non-invasive tear film break-up time (NIBUT)). Thirty dogs (54 eyes) were included in the final analysis based on the criteria. The NIBUT and tear film break-up time (TFBUT) results of the eyes included in the study were divided into three groups: Group 1 (&amp;lt; 5\u2009s), Group 2 (5 to &amp;lt;10\u2009s), and Group 3 (\u2265 10\u2009s). The Schirmer\u2019s tear Test 1 (STT-1) results of the included patients were also divided into three groups: Group 1 (&amp;lt; 5\u2009mm\/min), Group 2 (5 to &amp;lt;10\u2009mm\/min), and Group 3 (\u2265 10\u2009mm\/min). The corneal opacity grades are divided into four scores, ranging from 0 to 3. The corneal opacity grade score (COS) of 0 indicates a completely clear cornea or only a trace of opacity. COS of 1, 2, 3 indicate the presence of a prominent corneal opacity that does not interfere with the visualization of the fine iris details, the opacity obscures the visibility of the iris and lens details and severe obstruction of the intraocular structure visibility, respectively. The mean difference in COS during the follow-ups for each group of NIBUT were 0.61\u2009\u00b1\u20090.92 (<jats:italic>n<\/jats:italic>\u2009=\u200928), 0.10\u2009\u00b1\u20090.32 (<jats:italic>n<\/jats:italic>\u2009=\u200910), 0.19\u2009\u00b1\u20090.40 (<jats:italic>n<\/jats:italic>\u2009=\u200916). The NIBUT groups were significantly correlated with COS (<jats:italic>p<\/jats:italic>-value\u2009=\u20090.073) at a 10% level of significance. Post-hoc test at a 10% level of significance revealed significant correlations between Groups 1 and 2 (<jats:italic>p<\/jats:italic>-value\u2009=\u20090.041) and between Groups 1 and 3 (<jats:italic>p<\/jats:italic>-value 0.104). Although the TFBUT and STT-1 groups did not show any significant correlation with COS. Eyes with NIBUT of &amp;lt;5\u2009s were found to have a significantly higher chance of increased COS compared with eyes with NIBUT of &amp;gt;5\u2009s in the grid-line illumination plate NIBUT test. Among NIBUT, STT-1, and TFBUT, NIBUT was the only test that showed significant associations with the changes in COS.<\/jats:p>","DOI":"10.3389\/fvets.2024.1298467","type":"journal-article","created":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T05:00:36Z","timestamp":1712552436000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["A novel method of evaluating the non-invasive tear film break-up time and progression of corneal opacification in dogs using imaging video"],"prefix":"10.3389","volume":"11","author":[{"given":"Suk Jun","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myeong Gyun","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su-Jung","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun-Soo","family":"Choi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joon Young","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2024,4,8]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1016\/j.jtos.2017.05.008","article-title":"TFOS DEWS II definition and classification report","volume":"15","author":"Craig","year":"2017","journal-title":"Ocul Surf"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"589","DOI":"10.3390\/diagnostics10080589","article-title":"Novel approaches for imaging-based diagnosis of ocular surface 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