{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T05:44:25Z","timestamp":1784958265679,"version":"3.55.0"},"reference-count":68,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T00:00:00Z","timestamp":1708473600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>With the goal to maximize intake of high-fermentable diet needed to meet energy needs during weaning period, calves are at risk for ruminal acidosis. Using the calves from previously established model of feed-induced, ruminal acidosis in young calves, we aimed to investigate the changes in rumen epimural transcriptome and its microbial metatranscriptome at weaning (8-week) and post-weaning (17-week) in canulated (first occurred at 3 weeks of age) Holstein bull calves with feed-induced subacute ruminal acidosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eight bull calves were randomly assigned to acidosis-inducing diet (Treated, <jats:italic>n<\/jats:italic>\u2009=\u20094; pelleted, 42.7% starch, 15.1% neutral detergent fiber [NDF], and 57.8% nonfiber carbohydrates), while texturized starter was fed as a control (Control, <jats:italic>n<\/jats:italic>\u2009=\u20094; 35.3% starch, 25.3% NDF, and 48.1% nonfiber carbohydrates) starting at 1 week through 17 weeks. Calves fed acidosis-inducing diet showed significantly less (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.01) body weight over the course of the experiment, in addition to lower ruminal pH (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.01) compared to the control group. Rumen epithelial (RE) tissues were collected at both 8 weeks (via biopsy) and 17 weeks (via euthanasia) and followed for whole transcriptome RNA sequencing analysis. Differentially expressed genes (DEGs) analysis was done using cufflinks2 (fold-change \u22652 and <jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05) between treated and control groups at 8-week of age, and between 8- and 17-week for the treated group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At 8-week of age, DEGs between treatment groups showed an enrichment of genes related to the response to lipopolysaccharide (LPS) (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.005). The impact of prolonged, feed-induced acidosis was reflected by the decreased expression (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.005) in genes involved in cell proliferation related pathways in the RE at 17-week of age in the treated group. Unique sets of discriminant microbial taxa were identified between 8-and 17-week calves in the treated group and the treatment groups at 8-week, indicating that active microbial community changes in the RE are an integral part of the ruminal acidosis development and progression.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fvets.2024.1328539","type":"journal-article","created":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T04:28:46Z","timestamp":1708489726000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Investigating the impact of feed-induced, subacute ruminal acidosis on rumen epimural transcriptome and metatranscriptome in young calves at 8- and 17-week of age"],"prefix":"10.3389","volume":"11","author":[{"given":"Wenli","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Larsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscila","family":"Fregulia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,2,21]]},"reference":[{"key":"ref1","first-page":"47","article-title":"Effect of sodium butyrate supplementation in milk replacer and starter diet on rumen development in calves","volume":"60","author":"Gorka","year":"2009","journal-title":"J Physiol Pharmacol"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"7603","DOI":"10.3168\/jds.2013-6700","article-title":"Increased papillae growth and enhanced short-chain fatty acid absorption in the rumen of goats are associated with transient increases in 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