{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T20:36:37Z","timestamp":1780605397217,"version":"3.54.1"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T00:00:00Z","timestamp":1718755200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Mineral elements play a crucial role in supporting the life activities and physiological functions of animals. However, numerous studies have revealed that in some geographical areas and certain grazing situations, grazing livestock frequently suffers from mineral element deficiencies due to the loss of mineral elements from grassland forages, such as selenium (Se). To shed fresh light on this issue, this study aims to investigate the impact of dietary Se deficiency and supplementation on the liver of grazing sheep in these challenging conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>This study involved 28 grazing Mongolian Wu Ranke sheep with an average body weight of about 32.20\u2009\u00b1\u20090.37\u2009kg, which were divided into the Se treatment group and the control group. The Se treatment group was fed with the low-Se diet for 60\u2009days and then continued to be fed with the high-Se diet for 41\u2009days. The liver concentration of minerals, transcriptomic analysis, and untargeted metabolomic analysis were conducted to assess the impact of Se deficiency and supplementation on the liver of grazing sheep.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Dietary Se deficiency and supplementation significantly reduced and elevated liver concentration of Se, respectively (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05). Gene functional enrichment analysis suggested that dietary Se deficiency might impair protein synthesis efficiency, while Se supplementation was found to enhance liver protein synthesis in grazing sheep. <jats:italic>AGAP1<\/jats:italic>, <jats:italic>ERN1<\/jats:italic>, <jats:italic>MAL2<\/jats:italic>, <jats:italic>NFIC<\/jats:italic>, and <jats:italic>RERG<\/jats:italic> were identified as critical genes through the weighted gene correlation network analysis, the quantitative real-time polymerase chain reaction, and the receiver operating characteristic curve validation that could potentially serve as biomarkers. Metabolomics analysis revealed that dietary Se deficiency significantly reduced the abundance of metabolites such as 5-hydroxytryptamine, while dietary Se supplementation significantly elevated the abundance of metabolites such as 5-hydroxytryptophan (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Integrative analysis of the transcriptome and metabolome revealed that dietary Se deficiency led to reduced hepatic antioxidant and anti-inflammatory capacity, whereas Se supplementation increased the hepatic antioxidant and anti-inflammatory capacity in grazing Wu Ranke sheep. These findings provide new insights into the effects of dietary Se deficiency and supplementation on the liver of grazing sheep, potentially leading to improved overall health and well-being of grazing livestock.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fvets.2024.1358975","type":"journal-article","created":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T13:28:34Z","timestamp":1718890114000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Effects of dietary selenium deficiency and supplementation on liver in grazing sheep: insights from transcriptomic and metabolomic analysis"],"prefix":"10.3389","volume":"11","author":[{"given":"Xiwei","family":"Jin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingbo","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Mi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,6,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.jes.2020.06.024","article-title":"Gangba sheep in the Tibetan plateau: validating their unique meat quality and grazing factor analysis","volume":"101","author":"Zhang","year":"2021","journal-title":"J Environ Sci (China)"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"218","DOI":"10.5713\/ab.22.0252","article-title":"Iron sulfate and molasses treated anthocyanin-rich black cane silage improves growth performance, rumen fermentation, antioxidant status, and meat tenderness in goats","volume":"36","author":"Purba","year":"2023","journal-title":"Anim Biosci"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"44","DOI":"10.5713\/ajas.18.0955","article-title":"Relationship of mineral elements in sheep grazing in the highland agro-ecosystem","volume":"33","author":"Fan","year":"2020","journal-title":"Asian Australas J Anim Sci"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"1766","DOI":"10.2527\/jas.2016.1085","article-title":"Reducing mineral usage in feedlot diets for Nellore cattle: II. 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