{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,8]],"date-time":"2026-11-08T02:24:16Z","timestamp":1794104656113,"version":"4.8.0"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T00:00:00Z","timestamp":1712707200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p><jats:italic>Nocardia seriolae<\/jats:italic> is the primary pathogen causing nocardiosis in various fish species, leads to significant economic losses in the aquaculture industry. In this study, 10 bacterial strains isolated from <jats:italic>Micropterus salmoides<\/jats:italic> and <jats:italic>Channa argus<\/jats:italic> infected with nocardiosis, were identified as <jats:italic>N. seriolae<\/jats:italic> by physiological and biochemical identification, as well as 16S rDNA sequencing. Moreover, the key virulence-related genes such as ESX-1, T7SS-2, T7SS-3, EspG1, sodC, sod2 and ESAT6 were all positive, and showing high homology among different strains. Pathogenicity testing revealed mortality rates ranging from 70 to 100%, accompanied by the presence of white nodules in the viscera of deceased fish. The drug sensitivity test demonstrated that LY21811, the most lethal strain, exhibited high sensitivity to nine types of antibiotics, including azithromycin, doxycycline, florfenicol and compound sulfamethoxazole, yet showed complete resistance to \u03b2-lactam antibiotics. Additionally, the tannic acid also demonstrated potent inhibitory effects against LY21811, with a minimum inhibitory concentration of 0.0625\u2009mg\/mL. These results showed that <jats:italic>N. seriolae<\/jats:italic> originated from <jats:italic>M. salmoides<\/jats:italic> and <jats:italic>C. argus<\/jats:italic> in Zhejiang Province were highly conserved, demonstrating a high homogeneity in genetic characteristics, pathogenicity and antimicrobial susceptibilities. These results provide a foundation for further research on the pathogenic characteristics and disease prevention of <jats:italic>N. seriolae<\/jats:italic> infections.<\/jats:p>","DOI":"10.3389\/fvets.2024.1367066","type":"journal-article","created":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T11:25:18Z","timestamp":1712748318000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["Biological characteristics and pathogenicity comparison of Nocardia seriolae isolated from Micropterus salmoides and Channa argus"],"prefix":"10.3389","volume":"11","author":[{"given":"Wei","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kexin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyun","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayun","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxin","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyu","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyi","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,4,10]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1111\/jfd.12782","article-title":"Current knowledge of Nocardiosis in teleost fish","volume":"41","author":"Maekawa","year":"2018","journal-title":"J Fish Dis"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"778915","DOI":"10.3389\/fimmu.2022.778915","article-title":"Molecular identification of Nocardia seriolae and comparative analysis of spleen transcriptomes of hybrid snakehead (Channa maculata female \u00d7 Channa argus male) with Nocardiosis disease","volume":"13","author":"Zhang","year":"2022","journal-title":"Front Immunol"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1745","DOI":"10.1111\/jfd.12881","article-title":"Nocardia seriolae infection in the cultured eel Anguilla japonica in Korea","volume":"41","author":"Kim","year":"2018","journal-title":"J Fish Dis"},{"key":"ref4","first-page":"47","article-title":"The study of a tuberculosis-like condition in neon tetras (Hyphessobrycon innesi). 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