{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T04:10:05Z","timestamp":1793679005888,"version":"4.5.1"},"reference-count":83,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T00:00:00Z","timestamp":1715558400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>\n                    This study investigated the antioxidant effect of quercetin-treated semen on frozen\u2013thawed spermatozoa quality and\n                    <jats:italic>in-vivo<\/jats:italic>\n                    fertility in crossbred Kamori goats. In total, 32 ejaculates from four fertile bucks were diluted in Tris-based egg yolk extender with varying levels of quercetin (0, 1, 5, 10, and 15\u2009\u03bcM). Qualified semen samples were pooled and frozen in French straws. The results revealed that the addition of quercetin in the semen extender increased (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&amp;lt;\u20090.05) frozen\u2013thawed sperm total motility (TM), progressive motility (PM), rapid velocity (RV), average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), and amplitude of lateral head (ALH) displacement in contrast to the control group. Quercetin supplementation had no effect on beat cross frequency (BCF), straightness (STR), and linearity (LIN) (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&amp;gt;\u20090.05). Quercetin showed significantly higher (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&amp;lt;\u20090.05) plasma membrane and acrosome integrity and viability (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&amp;lt;\u20090.05) of spermatozoa in contrast to the control group. Quercetin in the semen extender significantly increased (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&amp;lt;\u20090.05) superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), and total antioxidant capacity (TAC) levels while reduced (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&amp;lt;\u20090.05) the contents of total oxidant status (TOS) and malondialdehyde (MDA), which were in contrast to the control group. Ultrasound results revealed that 24 out of 30 (80%) goats were found pregnant when semen was treated with 5\u2009\u03bcM quercetin while the control group showed 18 out of 30 (60%) animals were pregnant. Thus, the study concluded that 5\u2009\u03bcM quercetin-treated semen was found to be efficient, showed increased antioxidant status, and reduced oxidant production, leading to improved spermatozoa quality and\n                    <jats:italic>in-vivo<\/jats:italic>\n                    fertility in goats.\n                  <\/jats:p>","DOI":"10.3389\/fvets.2024.1385642","type":"journal-article","created":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T00:58:11Z","timestamp":1715561891000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["Quercetin in semen extender improves frozen-thawed spermatozoa quality and in-vivo fertility in crossbred Kamori goats"],"prefix":"10.3389","volume":"11","author":[{"given":"Iqra","family":"Batool","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Hammad","family":"Fayyaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amjad","family":"Hameed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed Murtaza Hassan","family":"Andrabi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rehana","family":"Kausar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Shahzad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasin","family":"Mubashir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali Dogan","family":"Omur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghulam","family":"Murtaza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allah","family":"Ditta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarique","family":"Hussain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,5,13]]},"reference":[{"key":"ref1","first-page":"177","article-title":"Artificial insemination in beetal and jattal goats: preliminary results","volume-title":"Sustainable goat farming for livelihood improvement in South Asia","author":"Andrabi","year":"2018"},{"key":"ref2","first-page":"307","article-title":"Artificial insemination: a tool to improve livestock productivity","volume":"6","author":"Kumar Patel","year":"2017","journal-title":"J Pharmacogn Phytochem"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1628","DOI":"10.1093\/humrep\/der132","article-title":"The role of sperm oxidative stress in male infertility and the significance of oral antioxidant therapy","volume":"26","author":"Gharagozloo","year":"2011","journal-title":"Hum Reprod"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1186\/1477-7827-12-45","article-title":"Relationship amongst teratozoospermia, seminal oxidative stress and male infertility","volume":"12","author":"Agarwal","year":"2014","journal-title":"Reprod Biol Endocrinol"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1007\/s10815-014-0425-7","article-title":"Contemporary evidence on the physiological role of reactive oxygen species in human sperm function","volume":"32","author":"Du Plessis","year":"2015","journal-title":"J Assist Reprod Genet"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1002\/mrd.1080350313","article-title":"Analysis of lipid peroxidation mechanisms in human spermatozoa","volume":"35","author":"Aitken","year":"1993","journal-title":"Mol Reprod Dev"},{"key":"ref7","first-page":"46","article-title":"The protective effect of antioxidants on liquid and frozen stored ram semen \u2013 review","volume":"47","author":"Budai","year":"2014","journal-title":"Anim Sci Biotechnol"},{"key":"ref8","first-page":"157","article-title":"Effect of vitamins C or E supplementation to Tris diluent on the semen quality of Awassi rams preserved at 5 \u00b0C","volume":"4","author":"Azawi","year":"2013","journal-title":"Vet Res Forum"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"1112","DOI":"10.1016\/j.theriogenology.2008.12.004","article-title":"McCorkell banking north American buffalo semen","volume":"71","author":"Lessard","year":"2009","journal-title":"Theriogenology"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/j.anireprosci.2018.03.005","article-title":"Effect of reduced glutathione supplementation on cryopreservation induced sperm cryoinjuries in Murrah bull semen","volume":"192","author":"Gangwar","year":"2018","journal-title":"Anim Reprod Sci"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1016\/S0093-691X(03)00050-5","article-title":"Subtle membrane changes in cryopreserved bull semen in relation with sperm viability, chromatin structure, and field fertility","volume":"60","author":"Januskauskas","year":"2003","journal-title":"Theriogenology"},{"key":"ref12","first-page":"1698","article-title":"Effects of frozen diluents on storage of ram sperm","volume":"1","author":"Soltanpour","year":"2013","journal-title":"Int J Adv Biol Bio Res"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12917-019-2135-y","article-title":"Effect of temperature and time after collection on buck sperm quality","volume":"15","author":"Hahn","year":"2019","journal-title":"BMC Vet Res"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"459","DOI":"10.5194\/aab-61-459-2018","article-title":"The effects of natural mating and artificial insemination using cryopreserved buck semen on reproductive performance in Alpine goats","volume":"61","author":"Agossou","year":"2018","journal-title":"Arch Anim Breed"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/j.theriogenology.2022.05.027","article-title":"Study on cryopreservation of Guanzhong dairy goat semen with bovine semen seminal plasma","volume":"189","author":"Zou","year":"2022","journal-title":"Theriogenology"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/j.smallrumres.2005.02.015","article-title":"A review on goat sperm cryopreservation","volume":"63","author":"Purdy","year":"2006","journal-title":"Small Rumin Res"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/S0378-4320(00)00156-1","article-title":"Production and storage of goat semen for artificial insemination","volume":"62","author":"Leboeuf","year":"2003","journal-title":"Anim Reprod Sci"},{"key":"ref18","first-page":"369","article-title":"Effect of seminal plasma, egg yolk, and season on the freezability of Saanen buck semen","volume":"53","author":"Ustuner","year":"2009","journal-title":"Bull Vet Inst Pulawy"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1016\/j.ejphar.2008.03.008","article-title":"Health effects of quercetin: from antioxidant to nutraceutical","volume":"585","author":"Boots","year":"2008","journal-title":"Eur J Pharmacol"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1111\/j.1439-0272.2010.01063.x","article-title":"Antioxidative potential of quercetin against hydrogen peroxide induced oxidative stress in spermatozoa in vitro","volume":"43","author":"Ben Abdallah","year":"2011","journal-title":"Andrologia"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"1001","DOI":"10.1016\/j.theriogenology.2012.06.032","article-title":"Quercetin improves the postthaw characteristics of cryopreserved sex-sorted and nonsorted stallion sperm","volume":"79","author":"Gibb","year":"2013","journal-title":"Theriogenology"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.1002\/ptr.3100","article-title":"Beneficial effects of quercetin on sperm parameters in streptozotocin-induced diabetic male rats","volume":"24","author":"Khaki","year":"2010","journal-title":"Phytother Res"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1089\/cpt.2006.4.169","article-title":"Effect of quercetin on capacitation status and lipid peroxidation of stallion spermatozoa","volume":"4","author":"McNiven","year":"2006","journal-title":"Cell Preserv Technol"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.tifs.2016.07.004","article-title":"The biological activities, chemical stability, metabolism and delivery systems of quercetin: a review","volume":"56","author":"Wang","year":"2016","journal-title":"Trends Food Sci Technol"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.4061\/2011\/686137","article-title":"Impacts of oxidative stress and antioxidants on semen functions","volume":"2011","author":"Bansal","year":"2011","journal-title":"Vet Med Int"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"437","DOI":"10.2164\/jandrol.109.007849","article-title":"Protective effects of ascorbate and catalase on human spermatozoa during cryopreservation","volume":"31","author":"Li","year":"2010","journal-title":"J Androl"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1002\/j.1939-4640.2005.tb02867.x","article-title":"Survival and in vitro fertility of boar spermatozoa frozen in the presence of superoxide dismutase and\/or catalase","volume":"26","author":"Roca","year":"2005","journal-title":"J Androl"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.cryobiol.2010.12.001","article-title":"Reduced glutathione content in human sperm is decreased after cryopreservation: Effect of the addition of reduced glutathione to the freezing and thawing extenders","volume":"62","author":"Gadea","year":"2011","journal-title":"Cryobiology"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"1149","DOI":"10.1016\/j.fertnstert.2010.10.005","article-title":"Vitamin E supplementation in semen-freezing medium improves the motility and protects sperm from freeze-thaw-induced DNA damage","volume":"95","author":"Kalthur","year":"2011","journal-title":"Fertil Steril"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"1722","DOI":"10.1016\/j.theriogenology.2011.11.023","article-title":"Effect of antioxidants resveratrol and quercetin on in vitro evaluation of frozen ram sperm","volume":"77","author":"Silva","year":"2012","journal-title":"Theriogenology"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"1237","DOI":"10.1590\/1678-4162-8670","article-title":"High resveratrol or quercetin concentrations reduce the oscillation index of frozen goat semen","volume":"68","author":"Silva","year":"2016","journal-title":"Arq Bras Med Vet Zootec"},{"key":"ref32","first-page":"298","article-title":"Efficacy of short-term estrus synchronization protocol and timed artificial insemination in subtropical goats","volume":"25","author":"Andrabi","year":"2015","journal-title":"J Anim Plant Sci"},{"key":"ref33","first-page":"157","article-title":"Estrus synchronization and artificial insemination in goats during low breeding season-A preliminary study","volume":"31","author":"Mehmood","year":"2010","journal-title":"Pak Vet J"},{"key":"ref34","first-page":"227","article-title":"Genetic resources and diversity in Pakistani goats","volume":"10","author":"Khan","year":"2008","journal-title":"Int J Agric Biol"},{"key":"ref35","doi-asserted-by":"publisher","first-page":"723","DOI":"10.2527\/1996.744723x","article-title":"Breeding season and aspects of reproduction of female goats","volume":"74","author":"Amoah","year":"1996","journal-title":"J Anim Sci"},{"key":"ref36","first-page":"433","article-title":"Estrus synchronization and conception rates using locally prepared methylacetoxyprogesterone sponges in cyclic and acyclic Nili-Ravi buffaloes (Bubalus bubalis)","author":"Kausar","year":"2013"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"700957","DOI":"10.3389\/fnut.2021.700957","article-title":"Moringa oleifera supplementation improves antioxidant status and biochemical indices by attenuating early pregnancy stress in Beetal goats","volume":"8","author":"Afzal","year":"2021","journal-title":"Front Nutr"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"708","DOI":"10.1111\/rda.14340","article-title":"Association of faecal volatiles and steroids with behavioural expression during oestrous cycle of sheep (Ovis aries)","volume":"58","author":"Yaseen","year":"2023","journal-title":"Reprod Domest Anim"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.smallrumres.2016.09.002","article-title":"In vitro viability and longevity of cooled Beetal buck spermatozoa extended in skimmed milk and Tris-citric acid based extenders","volume":"143","author":"Andrabi","year":"2016","journal-title":"Small Rumin Res"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/j.cryobiol.2012.12.010","article-title":"Effects of different concentrations of BHT on microscopic and oxidative parameters of Mahabadi goat semen following the freeze\u2013thaw process","volume":"66","author":"Naijian","year":"2013","journal-title":"Cryobiology"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1002\/j.1939-4640.2000.tb02116.x","article-title":"CASA\u2014practical aspects","volume":"21","author":"Mortimer","year":"2000","journal-title":"J Androl"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"e12733","DOI":"10.1111\/and.12733","article-title":"Programmable fast-freezing method improves the post-thaw motion dynamics, integrities of plasmalemma, mitochondrial transmembrane, DNA and, acrosome, and in vivo fertility of water buffalo (Bubalus bubalis) spermatozoa","volume":"49","author":"Khalil Ur Rehman","year":"2017","journal-title":"Andrologia"},{"key":"ref43","doi-asserted-by":"publisher","first-page":"2335","DOI":"10.1007\/s10616-016-0027-6","article-title":"Cryoprotection synergism between glycerol and dimethyl sulfoxide improves the mitochondrial transmembrane potential, plasmalemma, acrosomal and DNA integrities, and in vivo fertility of water buffalo (Bubalus bubalis) spermatozoa","volume":"68","author":"Shah","year":"2016","journal-title":"Cytotechnology"},{"key":"ref44","doi-asserted-by":"publisher","first-page":"972","DOI":"10.1111\/andr.12214","article-title":"Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa","volume":"4","author":"Shah","year":"2016","journal-title":"Andrology"},{"key":"ref45","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1016\/0003-2697(87)90489-1","article-title":"Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions","volume":"161","author":"Beyer","year":"1987","journal-title":"Anal Biochem"},{"key":"ref46","first-page":"1033","article-title":"Gamma irradiation effects on seed germination and growth, protein content, peroxidase and protease activity, lipid peroxidation in desi and kabuli chickpea","volume":"40","author":"Hameed","year":"2008","journal-title":"Pak J Bot"},{"key":"ref47","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/S0021-9258(19)50881-X","article-title":"A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase","volume":"195","author":"Beers","year":"1952","journal-title":"J Biol Chem"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1093\/jxb\/32.1.93","article-title":"Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase","volume":"32","author":"Dhindsa","year":"1981","journal-title":"J Exp Bot"},{"key":"ref49","doi-asserted-by":"publisher","first-page":"58","DOI":"10.15835\/nbha3925881","article-title":"Antioxidant and oxidant levels of pepper (Capsicum annuum cv. \u00e2\u20ac~ Charlee\u00e2\u20ac\u2122) infected with pepper mild mottle virus","volume":"39","author":"Dikilitas","year":"2011","journal-title":"Not Bot Horti Agrobo"},{"key":"ref50","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1016\/j.theriogenology.2007.10.004","article-title":"Ovsynch synchronization and fixed-time insemination in goats","volume":"69","author":"Holtz","year":"2008","journal-title":"Theriogenology"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1038\/206039a0","article-title":"Use of progestagen-impregnated sponges inserted intravaginally or subcutaneously for the control of the oestrous cycle in the sheep","volume":"206","author":"Robinson","year":"1965","journal-title":"Nature"},{"key":"ref52","doi-asserted-by":"publisher","first-page":"700957","DOI":"10.3389\/fnut.2021.700957","article-title":"Moringa oleifera supplementation improves antioxidant status and biochemical indices by attenuating early pregnancy stress in Beetal goats","volume":"8","author":"Afzal","year":"2021","journal-title":"Front nutr"},{"key":"ref53","doi-asserted-by":"publisher","first-page":"1516","DOI":"10.1016\/j.theriogenology.2016.05.010","article-title":"Semen quality parameters as fertility predictors of water buffalo bull spermatozoa during low-breeding season","volume":"86","author":"Ahmed","year":"2016","journal-title":"Theriogenology"},{"key":"ref54","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2022\/8282387","article-title":"Glycine improved cryopreserved spermatozoa quality in Achai bull","volume":"2022","author":"Nazif","year":"2022","journal-title":"Biomed Res Int"},{"key":"ref55","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.cryobiol.2017.03.002","article-title":"Antioxidant effect of quercetin in an extender containing DMA or glycerol on freezing capacity of goat semen","volume":"75","author":"Seifi-Jamadi","year":"2017","journal-title":"Cryobiology"},{"key":"ref56","doi-asserted-by":"publisher","first-page":"36","DOI":"10.21929\/abavet2021.36","article-title":"Evaluation of the quercetin and vitamin E addition to the cryopreservation medium of sheep semen on in vivo fertility","volume":"11","author":"Jim\u00e9nez-Aguilar","year":"2021","journal-title":"Abanico Vet"},{"key":"ref57","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.theriogenology.2019.05.012","article-title":"Stimulating effects of quercetin (QUE) in tris citric acid extender on post thaw quality and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa","volume":"134","author":"Ahmed","year":"2019","journal-title":"Theriogenology"},{"key":"ref58","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1016\/j.ijantimicag.2005.09.002","article-title":"Antimicrobial activity of flavonoids","volume":"26","author":"Cushnie","year":"2005","journal-title":"Int J Antimicrob Agents"},{"key":"ref59","first-page":"75","article-title":"Involvement of intracellular calcium and src tyrosine-kinase in capacitation of cryopreserved bovine spermatozoa","volume":"12","author":"Satorre","year":"2010","journal-title":"InVet"},{"key":"ref60","doi-asserted-by":"publisher","first-page":"326","DOI":"10.1016\/j.cryobiol.2012.09.003","article-title":"Effect of freezing\u2013thawing process and quercetin on human sperm survival and DNA integrity","volume":"65","author":"Zribi","year":"2012","journal-title":"Cryobiology"},{"key":"ref61","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1007\/978-3-319-30567-7_5","article-title":"Sperm capacitation and acrosome reaction in mammalian sperm","volume":"220","author":"Stival","year":"2016","journal-title":"Adv Anat Embryol Cell Biol"},{"key":"ref62","doi-asserted-by":"publisher","first-page":"604460","DOI":"10.3389\/fvets.2020.604460","article-title":"Effect of quercetin supplementation in extender on sperm kinematics, extracellular enzymes release, and oxidative stress of Egyptian buffalo bulls frozen\u2013thawed semen","volume":"7","author":"El-Khawagah","year":"2020","journal-title":"Front Vet Sci"},{"key":"ref63","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1089\/bio.2020.0007","article-title":"Effect of quercetin on oxidative stress, mitochondrial activity, and quality of Indian red jungle fowl (Gallus gallus murghi) sperm","volume":"18","author":"Rakha","year":"2020","journal-title":"Biopreserv Biobank"},{"key":"ref64","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1002\/j.1939-4640.1988.tb01020.x","article-title":"The sperm chromatin structure assay relationship with alternate tests of semen quality and heterospermic performance of bulls","volume":"9","author":"Ballachey","year":"1988","journal-title":"J Androl"},{"key":"ref65","doi-asserted-by":"publisher","first-page":"955","DOI":"10.1016\/S0093-691X(98)00199-X","article-title":"The relationship of increased susceptibility of sperm DNA to denaturation and fertility in the stallion","volume":"50","author":"Love","year":"1998","journal-title":"Theriogenology"},{"key":"ref66","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1016\/j.theriogenology.2023.10.022","article-title":"Cerium oxide nanoparticles improve the post-thaw quality and in-vivo fertility of Beetal buck spermatozoa","volume":"214","author":"Khalique","year":"2024","journal-title":"Theriogenology"},{"key":"ref67","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1093\/toxsci\/kfl155","article-title":"Cytogenetic damage induced by acrylamide and glycidamide in mammalian cells: correlation with specific glycidamide-DNA adducts","volume":"95","author":"Martins","year":"2007","journal-title":"Toxicol Sci"},{"key":"ref68","doi-asserted-by":"publisher","first-page":"921","DOI":"10.1016\/S0093-691X(00)00402-7","article-title":"Effect of the knobbed acrosome defect in bovine sperm on IVF and embryo production","volume":"54","author":"Thundathil","year":"2000","journal-title":"Theriogenology"},{"key":"ref69","doi-asserted-by":"publisher","first-page":"162S","DOI":"10.1097\/01.AOG.0000533134.61739.52","article-title":"Cryopreservation promotes sperm DNA damage through oxidative stress [38N]","volume":"131","author":"Tran","year":"2018","journal-title":"Obstet Gynecol"},{"key":"ref70","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.cryobiol.2020.04.010","article-title":"Quercetin supplemented casein-based extender improves the post-thaw quality of rooster semen","volume":"94","author":"Appiah","year":"2020","journal-title":"Cryobiology"},{"key":"ref71","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1007\/978-1-62703-038-0_2","article-title":"Methods of sperm vitality assessment","volume":"927","author":"Moskovtsev","year":"2013","journal-title":"Methods Mol Biol"},{"key":"ref72","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1111\/j.1439-0531.2006.00746.x","article-title":"Effect of sperm cryopreservation on the European eel sperm viability and spermatozoa morphology","volume":"42","author":"Asturiano","year":"2007","journal-title":"Reprod Domest Anim"},{"key":"ref73","doi-asserted-by":"publisher","first-page":"81","DOI":"10.21608\/jsaes.2023.246737.1061","article-title":"Visual examination and computer assessed sperm analysis (Casa) of goat semen diluted by Tris-extender supplemented with aqueous extract of Moringa Oleifera leaves","volume":"2","author":"Mahmoud","year":"2023","journal-title":"J Sust Agric Enviro Sci"},{"key":"ref74","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.cryobiol.2018.02.001","article-title":"Cryoprotective role of organic Zn and cu supplementation in goats (Capra hircus) diet","volume":"81","author":"Arangasamy","year":"2018","journal-title":"Cryobiology"},{"key":"ref75","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1016\/j.cryobiol.2020.09.002","article-title":"Effects of mint, thyme, and curcumin extract nanoformulations on the sperm quality, apoptosis, chromatin decondensation, enzyme activity, and oxidative status of cryopreserved goat semen","volume":"97","author":"Ismail","year":"2020","journal-title":"Cryobiology"},{"key":"ref76","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1111\/rda.12714","article-title":"Protective effects of quercetin on selected oxidative biomarkers in bovine spermatozoa subjected to ferrous ascorbate","volume":"51","author":"Tvrd\u00e1","year":"2016","journal-title":"Reprod Domest Anim"},{"key":"ref77","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1111\/jpn.13056","article-title":"Quercetin attenuates H2O2-induced toxicity of rooster semen during liquid storage at 4 C","volume":"103","author":"Ghaniei","year":"2019","journal-title":"J Anim Physiol Anim Nutr"},{"key":"ref78","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1093\/toxsci\/kfi333","article-title":"Protective effect of quercetin on Aroclor 1254\u2013induced oxidative damage in cultured chicken spermatogonial cells","volume":"88","author":"Zhang","year":"2005","journal-title":"Toxicol Sci"},{"key":"ref79","doi-asserted-by":"publisher","first-page":"e14167","DOI":"10.1111\/and.14167","article-title":"The effect of quercetin on the quality of sperm parameters in frozen-thawed semen of patients with Asthenospermia","volume":"53","author":"Cheraghi","year":"2021","journal-title":"Andrologia"},{"key":"ref80","doi-asserted-by":"publisher","first-page":"2840","DOI":"10.3390\/ani11102840","article-title":"Antioxidant and antiapoptotic effects of a turraea fischeri leaf extract on cryopreserved goat sperm","volume":"11","author":"Hassan","year":"2021","journal-title":"Animals"},{"key":"ref81","doi-asserted-by":"publisher","first-page":"18538","DOI":"10.1038\/srep18538","article-title":"High total antioxidant capacity of the porcine seminal plasma (SP-TAC) relates to sperm survival and fertility","volume":"5","author":"Barranco","year":"2015","journal-title":"Sci Rep"},{"key":"ref82","first-page":"95","article-title":"Fertilizing capacity of buck (Capra hircus) semen frozen with different concentrations of egg yolk","volume":"5","author":"Al-Faruque","year":"2007","journal-title":"J Bangladesh Agric Univ"},{"key":"ref83","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1089\/bio.2022.0024","article-title":"The proteomic modification of Buck ejaculated sperm induced by the cryopreservation process","volume":"21","author":"Lv","year":"2023","journal-title":"Biopreserv Biobank"}],"container-title":["Frontiers in Veterinary Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2024.1385642\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T00:58:19Z","timestamp":1715561899000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2024.1385642\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,13]]},"references-count":83,"alternative-id":["10.3389\/fvets.2024.1385642"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2024.1385642","relation":{},"ISSN":["2297-1769"],"issn-type":[{"value":"2297-1769","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,13]]},"article-number":"1385642"}}