{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:32:39Z","timestamp":1792801959166,"version":"4.5.0"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,4,5]],"date-time":"2024-04-05T00:00:00Z","timestamp":1712275200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>Baicalein (B) has potential antioxidant properties, but it has not been tested as a ram semen extender. This study aimed to assess the impact of B on various sperm parameters and determine its potential influence on semen quality after the freeze-thawing process. During the breeding season, ejaculates were obtained from four rams with the aid of an artificial vagina. The collected mixed semen samples were divided into four groups: control (C; 0), B0.5 (0.5\u2009mM), B1 (1\u2009mM), and B2 (2\u2009mM). After semen extension, the samples were loaded into 0.25\u2009mL straws and stored for 2\u2009h at 4\u00b0C prior to freezing in liquid nitrogen vapor and thawed in a water bath at 37\u00b0C. Among the groups, B0.5 demonstrated the highest progressive motility results, while B1 and B2 exhibited reduced motility (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05). In terms of high mitochondrial membrane potential, plasma membrane and acrosome integrity, and viability, B0.5 showed significantly superior outcomes to the other B groups (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05), although it was not significantly better than C. B1 displayed the highest plasma membrane integrity levels (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05). Notably, B2 displayed the lowest total antioxidant status levels among the groups (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05). The findings of this study suggested that the <jats:italic>in vitro<\/jats:italic> spermatological characteristics of ram spermatozoa such as progressive motility and chromatin integrity can be protected from the freeze-thawing process by using the 0.5\u2009mM dose of baicalein as a semen extender. The treatment of sperm freezing might benefit from further in-depth research on the role of B in the improvement of cryoinjury and its underlying processes.<\/jats:p>","DOI":"10.3389\/fvets.2024.1394273","type":"journal-article","created":{"date-parts":[[2024,4,5]],"date-time":"2024-04-05T13:03:10Z","timestamp":1712322190000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Cryopreservation of ram semen: baicalein efficiency on oxidative stress, chromatin integrity, viability and motility post thaw"],"prefix":"10.3389","volume":"11","author":[{"given":"Fatih","family":"Avdatek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u015e\u00fckr\u00fc","family":"G\u00fcng\u00f6r","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet 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