{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T09:07:43Z","timestamp":1765357663572,"version":"3.41.2"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,8,21]],"date-time":"2024-08-21T00:00:00Z","timestamp":1724198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:italic>Nocardia farcinica<\/jats:italic> is an opportunistic bacterium that causes bovine mastitis and pulmonary, cutaneous, and central nervous system infections in humans. Bovine abortion caused by <jats:italic>N. farcinica<\/jats:italic> has been sporadically reported. The purpose of this study was to analyze the pathological findings of bovine abortions caused by <jats:italic>N. farcinica<\/jats:italic> in the Republic of Korea and determine the antimicrobial resistance and genotypical characteristics of <jats:italic>N. farcinica<\/jats:italic> isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Case presentation<\/jats:title><jats:p>Three cases of bovine abortions were submitted to the Animal and Plant Quarantine Agency for differential diagnosis. Grossly, one fetus showed severe lung consolidation following palpation of the entire lobes. Histologically, necrotizing granulomatous interstitial pneumonia was observed in all fetuses; a fetus with a gross lesion demonstrated necrotizing lymphadenitis in the mesenteric lymph nodes and necrotizing dermatitis in the ear. <jats:italic>N. farcinica<\/jats:italic> isolates were isolated from the abomasal contents and lungs of all fetuses. Finally, two cases were diagnosed as abortions due to <jats:italic>N. farcinica<\/jats:italic>, and one was diagnosed as an <jats:italic>N. farcinica<\/jats:italic> abortion coinfected with bovine viral diarrhea virus. According to the multilocus sequence analysis, all isolates were identified as <jats:italic>N. farcinica<\/jats:italic> and were determined to be genetically related to isolates from humans. Two <jats:italic>N. farcinica<\/jats:italic> isolates were resistant to trimethoprim-sulfamethoxazole, which is recommended as the first treatment for human nocardial infections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This is the first pathological report of bovine abortion caused by <jats:italic>N. farcinica<\/jats:italic> in the Republic of Korea. Further studies are needed to phenotypically and genotypically characterize <jats:italic>N. farcinica<\/jats:italic> isolates with various sources and continuously monitor antimicrobial resistance patterns.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fvets.2024.1407634","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T22:04:36Z","timestamp":1724364276000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Case report: Pathology, antimicrobial resistance, and molecular characterization of bovine abortion cases caused by Nocardia farcinica in Korean native cattle"],"prefix":"10.3389","volume":"11","author":[{"given":"Eun-Mi","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi 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Rep"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1016\/j.cmi.2018.06.013","article-title":"Antibiotic susceptibility testing and species identification of Nocardia isolates: a retrospective analysis of data from a French expert laboratory, 2010\u20132015","volume":"25","author":"Lebeaux","year":"2019","journal-title":"Clin Microbiol Infect"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1128\/CMR.7.2.213","article-title":"Nocardia species: host-parasite relationships","volume":"7","author":"Beaman","year":"1994","journal-title":"Clin Microbiol Rev"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/j.vetmic.2007.04.044","article-title":"Phenotypic and molecular epidemiologic evaluation of a Nocardia farcinica mastitis epizootic","volume":"125","author":"Brown","year":"2007","journal-title":"Vet Microbiol"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1099\/jmm.0.46774-0","article-title":"Clinical and microbiological features of nocardiosis 1997\u20132003","volume":"56","author":"Mu\u00f1oz","year":"2007","journal-title":"J Med Microbiol"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1590\/s0036-46652008005000004","article-title":"Nocardiosis: an overview and additional report of 28 cases in cattle and dogs","volume":"50","author":"Ribeiro","year":"2008","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"1665","DOI":"10.3346\/jkms.2010.25.11.1665","article-title":"The first case of catheter-related bloodstream infection caused by Nocardia farcinica","volume":"25","author":"Heo","year":"2010","journal-title":"J Korean Med Sci"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"101","DOI":"10.4046\/trd.2016.79.2.101","article-title":"A case of Nocardia 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Microbiol"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1590\/S0036-46652008005000004","article-title":"Nocardiosis: an overview and additional report of 28 cases in cattle and dogs","volume":"50","author":"Ribeiro","year":"2008","journal-title":"Rev Inst Med Trop S Paulo"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/s0749-0720(15)31281-0","article-title":"Examination of bovine and ovine fetuses","volume":"2","author":"Kirkbride","year":"1986","journal-title":"Vet Clin North Am Food Anim Pract"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"428","DOI":"10.1177\/10406387241239919","article-title":"Laboratory investigation of causes of bovine abortion and stillbirth in the Republic of Korea, 2014\u20132020","volume":"36","author":"Kim","year":"2024","journal-title":"J Vet Diagn Invest"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"4059","DOI":"10.1128\/JCM.37.12.4059-4064.1999","article-title":"Detection by PCR of Neospora caninum in fetal tissues from spontaneous bovine abortions","volume":"37","author":"Baszler","year":"1999","journal-title":"J Clin Microbiol"},{"volume-title":"Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes","year":"2011","author":"Woods","key":"ref25"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"1665","DOI":"10.1292\/jvms.001665","article-title":"Bovine stillbirth due to Nocardia farcinica","volume":"71","author":"Saito","year":"2009","journal-title":"J Vet Med Sci"},{"key":"ref27","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1177\/104063871002200122","article-title":"Bovine abortion associated with Nocardia farcinica","volume":"22","author":"Bawa","year":"2010","journal-title":"J Vet Diagn Invest"},{"key":"ref28","first-page":"195","article-title":"Bovine abortion due to Nocardia asteroides\u2014a first case report in Tabora","volume":"28","author":"Mugarula","year":"1980","journal-title":"Bull Anim Health Prod Afr"},{"key":"ref29","first-page":"273","article-title":"Bovine abortion associated with Nocardia asteroides","volume":"161","author":"Wohlgemuth","year":"1972","journal-title":"J Am Vet Med Assoc"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"e0156021","DOI":"10.1128\/spectrum.01560-21","article-title":"Epidemiology and antimicrobial resistance profiles of the Nocardia species in China, 2009 to 2021","volume":"10","author":"Wang","year":"2022","journal-title":"Microbiol Spectr"},{"key":"ref31","first-page":"370","article-title":"Isolation of Nocardia brasiliensis from a cat with a review of its prevalence and geographic distribution","volume":"138","author":"Ajello","year":"1961","journal-title":"J Am Vet Med 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