{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T16:15:46Z","timestamp":1784304946538,"version":"3.55.0"},"reference-count":21,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T00:00:00Z","timestamp":1729814400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>Since 2020, a novel goose-derived reovirus, characterized by goose hemorrhagic hepatitis, has emerged in the goose breeding industry of Guangdong province, China, leading to significant economic losses in the poultry sector. To study the genetic variation of novel goose reovirus (NGRV) in Guangdong province, this experiment utilized goose embryonic fibroblast cells for virus isolation. RT-PCR was conducted to identify, amplify, clone, and sequence the complete genome of the NGRV isolated from Zhaoqing. The genomic sequences were compared with reference strains to construct a phylogenetic tree. Moreover, animal pathogenicity, excretion patterns, and pathological sections were examined. The results showed that liver and spleen samples from geese suspected of NGRV infection were used for isolation, resulting in the identification of a reovirus presumed to originate from geese, designated as GD218. In terms of genomic structure and sequence homology, GD218 closely resembles the novel duck reovirus, differing significantly from earlier isolated NDRV strains (J18, NP03, SD12, etc.) in genetic composition (nt: 80.6\u201397.9%, aa: 94.3\u201398.9%). However, it is similar to strains isolated after 2018, such as XT18, SY, QR, YL, LY20, etc. (nt: 95.3\u201398.9%, aa: 98.6\u201399.7%). Therefore, based on phylogenetic analysis, GD218 is hypothesized to be a novel type of goose-origin reovirus homologous to the novel duck reovirus.<\/jats:p>","DOI":"10.3389\/fvets.2024.1423122","type":"journal-article","created":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T13:45:34Z","timestamp":1729863934000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Isolation and identification of a novel goose-origin reovirus GD218 and its pathogenicity experiments"],"prefix":"10.3389","volume":"11","author":[{"given":"Yuze","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongkun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenghao","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Mei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujian","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,10,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"12185","DOI":"10.1128\/JVI.01667-09","article-title":"Multifaceted sequence-dependent and-independent roles for reovirus FAST protein cytoplasmic tails in fusion pore formation and syncytiogenesis","volume":"83","author":"Barry","year":"2009","journal-title":"J Virol"},{"key":"ref2","first-page":"18","article-title":"A new virus disease of the Muscovy-duck [Cairina moschata (linn)] present in Natal","volume":"21","author":"Kaschula","year":"1950","journal-title":"J S Afr Vet Assoc"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"3028","DOI":"10.1111\/tbed.14236","article-title":"Isolation and characterization of a new goose orthoreovirus causing liver and spleen focal necrosis in geese, China","volume":"69","author":"Huang","year":"2022","journal-title":"Transbound Emerg Dis"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"109620","DOI":"10.1016\/j.vetmic.2022.109620","article-title":"Recombinant characteristics, pathogenicity, and transmissibility of a variant goose orthoreovirus derived from inter-lineage recombination","volume":"277","author":"Zhang","year":"2023","journal-title":"Vet Microbiol"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"968","DOI":"10.1128\/jvi.19.3.968-976.1976","article-title":"Physical and chemical characterization of an avian reovirus","volume":"19","author":"Spandidos","year":"1976","journal-title":"J Virol"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.virol.2005.08.002","article-title":"Structure of avian orthoreovirus virion by electron cryomicroscopy and image reconstruction","volume":"343","author":"Zhang","year":"2005","journal-title":"Virology"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1016\/j.vetmic.2013.11.005","article-title":"Genomic characteristics of a novel reovirus from Muscovy duckling in China","volume":"168","author":"Yun","year":"2014","journal-title":"Vet Microbiol"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12917-019-1877-x","article-title":"Molecular characterization of two novel reoviruses isolated from Muscovy ducklings in Guangdong, China","volume":"15","author":"Zhang","year":"2019","journal-title":"BMC Vet Res"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1007\/s00705-014-2241-x","article-title":"Molecular characterization of a novel reovirus isolated from Pekin ducklings in China","volume":"160","author":"Zhu","year":"2015","journal-title":"Arch Virol"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"1006","DOI":"10.1128\/jvi.43.3.1006-1014.1982","article-title":"Avian reovirus polypeptides: analysis of intracellular virus-specified products, virions, top component, and cores","volume":"43","author":"Schnitzer","year":"1982","journal-title":"J Virol"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1099\/0022-1317-83-1-131","article-title":"Subunit composition and conformational stability of the oligomeric form of the avian reovirus cell-attachment protein \u03c3C","volume":"83","author":"Grande","year":"2002","journal-title":"J Gen Virol"},{"key":"ref12","first-page":"13","article-title":"Studies on chronic respiratory disease of chickens II. Isolation of a virus","volume":"18","author":"Fahey","year":"1954","journal-title":"Can J Comp Med Vet Sci"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1637\/0005-2086(2002)046[0032:IOARFP]2.0.CO;2","article-title":"Isolation of a reovirus from poult enteritis and mortality syndrome and its pathogenicity in turkey poults","volume":"46","author":"Heggen-Peay","year":"2002","journal-title":"Avian Dis"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"8001","DOI":"10.1016\/j.vaccine.2018.10.102","article-title":"Development of a live attenuated vaccine against Muscovy duck reovirus infection","volume":"36","author":"Chen","year":"2018","journal-title":"Vaccine"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"3113","DOI":"10.1016\/S0264-410X(02)00264-5","article-title":"Baculovirus-expressed Muscovy duck reovirus \u03c3C protein induces serum neutralizing antibodies and protection against challenge","volume":"20","author":"Kuntz-Simon","year":"2002","journal-title":"Vaccine"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"39092","DOI":"10.1038\/srep39092","article-title":"Induction of a robust immunity response against novel duck reovirus in ducklings using a subunit vaccine of sigma C protein","volume":"6","author":"Bi","year":"2016","journal-title":"Sci Rep"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"103764","DOI":"10.1016\/j.micpath.2019.103764","article-title":"Impacts of novel duck reovirus infection on the composition of intestinal microbiota of Muscovy ducklings","volume":"137","author":"Chen","year":"2019","journal-title":"Microb Pathog"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1186\/s12917-019-2090-7","article-title":"Specific detection and differentiation of classic goose parvovirus and novel goose parvovirus by TaqMan real-time PCR assay, coupled with host specificity","volume":"15","author":"Wan","year":"2019","journal-title":"BMC Vet Res"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"102776","DOI":"10.1016\/j.psj.2023.102776","article-title":"Molecular characterization, complete genome sequencing, and pathogenicity of Novel Duck Reovirus from South Coastal Area in China","volume":"102","author":"Kong","year":"2023","journal-title":"Poult Sci"},{"key":"ref20","first-page":"346","article-title":"Isolation and identification of avian viral arthritis virus[J]","author":"Chen","year":"1991","journal-title":"J. Virol"},{"key":"ref21","article-title":"Preparation of inactivated oil emulsion vaccine of goose reovirus[D]","author":"Zhang","year":"2017"}],"container-title":["Frontiers in Veterinary Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2024.1423122\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T13:45:36Z","timestamp":1729863936000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2024.1423122\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,25]]},"references-count":21,"alternative-id":["10.3389\/fvets.2024.1423122"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2024.1423122","relation":{},"ISSN":["2297-1769"],"issn-type":[{"value":"2297-1769","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,25]]},"article-number":"1423122"}}